Nimet FURUCU, Leman SENCAR, M.turan ÇETİN, İbrahim Ferhat ÜRÜNSAK, Derya GÜMÜRDÜLÜ, Sait POLAT

Endometriotik dokularda interlökin-6 ve tümör nekroz faktör alfa ekspresyonu: bir immünohistokimyasal çalışma

Amaç: Bu çalışmada, insanda normal endometriyum ve endometriyotik dokularda Tümör nekroz faktör-α (TNF-α) ve Interlökin-6 (IL-6) ekspresyonlarının immünohistokimyasal yöntemler ile araştırılması amaçlandı. Gereç ve Yöntem: Çalışmada incelenen doku kesitleri, 20-40 yaşları arasındaki 24 kadından alınan ektopik endometriyum doku biyopsilerinden elde edildi. Ayrıca herhangi bir endometriyal fonksiyon bozukluğu bulunmayan, ancak diletasyon veya küretaj nedeniyle, 10 hastadan elde edilen endometriyum dokularından alınan kesitler de kontrol grubu olarak değerlendirildi. Işık mikroskobik ve immünohistokimyasal yöntemler ile hazırlanan doku kesitleri, ışık mikroskopta incelendi. Bulgular: TNF-α ve IL-6 ekspresyonunun kontrol grubunda yüzey ve bez epitelinde zayıftan orta dereceye kadar değişen düzeylerde olduğu gözlendi. Buna karşın stromal hücrelerde belirgin bir boyanmaya rastlanmadı. TNF-α ve IL-6'nın endometriyotik dokulardaki immünreaktivitesinin kontrol endometriyum ile karşılaştırıldığında, epitel hücreleri ile stromal hücreler ve makrofajlarda belirgin olarak artış gösterdiği bulundu. TNF-α ve IL-6 ekspresyonlarının her ikisi de ektopik endometriyal dokuda, güçlü olarak eksprese olmuştur. Sonuç: TNF-α ve IL-6’nın endometriyozis oluşumu ve patogenezinde rol alan önemli sitokinler oldukları kanaatine varıldı.

Expression of interleukin 6 and tumor necrosis factor alpha in endometriotic tissues: an immunohistochemical study

Purpose: The aim of this study is to investigate Tumor necrosis factor alpha (TNF-α) and Interleukin 6 (IL-6) expressions in normal and endometriotic human tissue using immunohistochemical methods.Materials and Methods: This study examined tissue sections obtained from the tissue biopsies of ectopic endometrium taken from 24 women between the ages of 20 to 40 years. Additionally, the tissue sections taken from the normal endometrium obtained from 10 patients without any endometrial dysfunction due to dilation or curettage were also evaluated, as the control group. Tissue sections prepared by light microscopic and immunohistochemical methods were examined under a light microscope.Results: In the control group TNF-α and IL-6 expressions were identified at varying levels, from weak to moderate, in surface and glandular epithelium; however, there was no evidence of a significant staining in the stromal cells. It is found that TNF-α and IL-6 immunoreactivity in the endometriotic tissues were significantly increased in the epithelial cells, stromal cells and macrophages compared to the control endometrium. Expressions of TNF-α and IL-6 were both strong in the ectopic endometrial tissues. Conclusion: We conclude that TNF-α and IL-6 are important cytokines involved in the pathogenesis of the endometriosis.

Keywords:

endometriosis, IL-6, TNF-α, immunohistochemistry,

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