Salih MAÇİN, Fatma Nur AKDOĞAN KITTANA, Yakut Akyön YILMAZ

Çeşitli klinik örneklerden izole edilen pseudomonas aeruginosa suşlarının virulans faktörlerinin incelenmesi

Amaç: Pseudomonas aeruginosa aerob, sporsuz, düz veya hafif kıvrımlı, 42 C° de üreyebilen Gram negatif basillerdir. P. aeruginosa; ürettiği virülans faktörlerinin çeşitliliği ve sürekli yükselen antibiyotik direnç oranlarıyla sık rastlanan, mortalite ve morbiditesi yüksek, tedavisi güç enfeksiyonlara neden olmaktadır. Bu çalışmadaki amacımız çeşitli klinik örneklerden izole edilen P. aeruginosa suşlarının virulans faktörlerini incelemektir. Gereç ve Yöntem: Hacettepe Üniversitesi Tıp Fakültesi Hastanesi, Mikrobiyoloji Laboratuvarı’nda hastalara ait örneklerden izole edilen ve P.aeruginosa olarak tanımlanan 258 suş çalışmaya dahil edildi. Fenotipik testler olarak DNaz, proteaz, elastaz, piyosiyanin, mukoid fenotip, hemoliz ve hareket testi çalışıldı. Bulgular: Çalışmaya dahil edilen suşların 190’ı (%73.6) elastaz, 170’i (%65.9) proteaz, 222’si (%86) hareket, 56’sı (%21.7) piyosiyanin, 205’i (%79.5) hemoliz, 67’si (%26) DNaz, 53’ü (%20.5) mukus varlığı açısından pozitif saptanmıştır. Sonuç: P. aeruginosa ürettiği virulans faktörlerinin çeşitliliği ve artan antibiyotik direnç oranlarıyla tedavisi güç enfeksiyonlara neden olmaktadır. Elde edilen verilerin yeni bir tedavi stratejisine yardımcı olabileceği düşünülmektedir.

Anahtar Kelimeler:

Pseudomonas aeruginosa, Virulans faktörleri, bakteriyemi.

Investigation of virulence factors of pseudomonas aeruginosa strains isolated from various clinical samples

Purpose: Pseudomonas aeruginosa is an aerobic, non-spore forming, straight or slightly curved, gram-negative bacilli and it can grow at 42 °C. Virulence factors of P. aeruginosa are various and its morbidity, mortality and antibiotic resistance rates are increasing constantly. The aim of this study was to examine the virulence factors of P. aeruginosa isolates that isolated from various sites of the body. Material and Method: Isolates of patients that were identified as P. aeruginosa were included into the study (n:258). Identification was done in Hacettepe University Hospital Microbiology Laboratory. DNase, protease, elastase, pyocyanin, mucoid phenotype, haemolysis and motility tests were performed as phenotypic tests. Results: Positivity rates of the virulance factors are; 190 (73.6%) elastase, 170 (65.9%) protease, 222 (86%) motility, 56 (21.7%) pyocyanin, 205 (79.5%) hemolysis, 67 'si (26%) DNase and 53 (20.5%) mucoid phenotyp, respectively. Conclusion: Since the diversity of virulence factor produced by P. aeruginosa and antibiotic resistance rate increase, it causes difficult to treat infections. The data will provide an insight into new approaches to treatment.

Keywords:

Pseudomonas aeruginosa, virulence factors, bacteraemia.,

PDF

___

1. Palleroni NJ. Prokaryote taxonomy of the 20th century and the impact of studies on the genus Pseudomonas: a personal view. Microbiology-Sgm. 2003;149:1-7.
2. Blanc DS, Petignat C, Janin B, Bile J, Francioli P. Frequency and molecular diversity of Pseudomo-nas aeruginosa upon admission and during hospi-talization: a prospective epidemiologic study. Clin Microbiol Infect. 1998;4:242-7.
3. Woods DE. Comparative genomic analysis of Pseudomonas aeruginosa virulence. Trends Microbiol. 2004;12:437-9.
4. Petermann SR, Doetkott C, Rust L. Elastase deficiency phenotype of Pseudomonas aeruginosa canine otitis externa isolates. Clin Diagn Lab Immunol. 2001;8:632-6.
5. Burke V, Robinson JO, Richardson CJL, Bundell CS. Longitudinal-studies of virulence factors of pseudomonas aeruginosa in cystic-fibrosis. pathology. 1991;23:145-8.
6. Alcorn JF,Wright JR. Degradation of pulmonary surfactant protein D by Pseudomonas aeruginosa elastase abrogates innate immune function. J Biol Chem. 2004;279:30871-9.
7. Hingley ST, Hastie AT, Kueppers F, Higgins ML. Disruption of respiratory cilia by proteases including those of pseudomonas aeruginosa. Infect Immun. 1986;54:379-85.
8. Çıragil P, Söyletir G. Çeşitli vücut bölgelerinden izole edilen Pseudomonas aeruginosa suşlarının aljinat elastaz ve alkali proteaz üretimleri. Mikrobiyol Bült. 2004;38:341-7.
9. Lau GW, Hassett DJ, Ran HM, Kong FS. The role of pyocyanin in Pseudomonas aeruginosa infection. Trends Mol Med. 2004;10:599-606.
10. Ohfuji K, Sato N, Hamada-Sato N, Kobayashi T, Imada C, Okuma H et al. Construction of a glucose sensor based on a screen-printed electrode and a novel mediator pyocyanin from Pseudomonas aeruginosa. Biosens Bioelectron. 2004;19:1237-44.
11. Schaber JA, Carty NL, McDonald NA, Graham ED, Cheluvappa R, Griswold JA et al. Analysis of quorum sensing-deficient clinical isolates of Pseudomonas aeruginosa. J Med Microbiol. 2004;53:841-53.
12. Cullen L, McClean S. Bacterial adaptation during chronic respiratory infections. Pathogens. 2015;4:66-89.
13. Fegan M, Francıs P, Hayward AC, Davıs G, Fuerst JA. Phenotypic conversion of pseudomonas aeruginosa in cystic fibrosis. J Clın Mıcrobiol. 1990;28:1143-6.
14. Estrellas PS, Alionte LG, Hobden JA. A Pseudomonas aeruginosa strain isolated from a Contact Lens-Induced Acute Red Eye (CLARE) is protease-deficient. Curr Eye Res. 2000;20:157-65.
15. Finlayson EA, Brown PD. Comparison of antibiotic resistance and virulence factors in pigmented and non-pigmented Pseudomonas aeruginosa. West Indian Med J. 2011;60:24-32.