IAA, 28-homobrassinolid ve 24-epibrassinolidin asmanın adventif köklenmesinin teşviki üzerine etkisi

Bu araştırmada oksin ve brassinosteroidlerin Amerikan asma anaçlarının (140 Ru ve 41 B) köklenmesi üzerine olan etkisi araştırılmıştır. Kullanılan hormonlar ve konsantrasyonları şöyledir: indol butirik asit (IBA) 0 (kontrol), 1000, 1500, 2000 ve 4000 ppm 5 saniyelik daldırma, 22(S),23(S)-homobrassinolid (HBR) ve 24- epibrassinolid (EBR); 0 (kontrol), 10, 0,25, 0,50 ve 1,00 ppm 10 dakikalık daldırma. İklim odasında büyümesine ve gelişmesine izin verilen çeliklerde önce sürme, köklenme ve sağlıklı bitki oranları hesaplanmıştır. Daha sonra, köklenen ve süren çeliklerde kök gelişimi (kök sayısı, en uzun kök uzunluğu, kök gelişimi derecesi, taze ve kuru kök ağırlıkları ile kuru kök oranı) v sürgün gelişimi (primer sürgün uzunluğu, boğum sayısı, sürgün sayısı ve koltuk sürgünü sayısı) belirlenmiştir. 140 Ru daima 41 B anacına göre daha iyi büyüme ve gelişme göstermiştir. IBA uygulamalarının her iki anacın büyümesi ve gelişmesi üzerine belirgin önemli etkileri tespit edilmemekle birlikte 5 saniye 1500 ppm IBA ve 10 dakika 1.00 ppm HBR uygulamaları 41 B anacında köklenmeyi ve sağlıklı bitki oranlarını teşvik ettiği gösterilmiştir. Diğer taraftan EBR herhangi bir başarı sağlamamıştır. 1000 ppm IBA, 0.50 ve 1.00 ppm HBR ve tüm EBR uygulamaları 140 Ru anacında dikkati çeken bir köklenme ve sağlık bitki oranı artışı vermiştir. Bu araştırma brassinosteroidlerin asma çeliklerinin köklendirilmesinde başarılı şekilde kullanılabileceğini göstermiştir.

Anahtar Kelimeler:

oksin, IBA, brassinosteroid, homobrassinolid, epibrassinolid, asma, anaç

Influence of IAA, 28-homobrassinolide and 24-epibrassinolide on promoting grapevine adventitious rooting

In this research, influence of auxin and brassinosteroids on rooting of American grapevine rootstocks (140 Ru and 41 B) was investigated. Hormones and their concentrations used were indole butyric acid (IBA); 0 (control), 1000, 1500, 2000 and 4000 ppm for 5-second dipping, 22(S),23(S)-homobrassinolide (HBR) and 24- epibrassinolide (EBR); 0 (control), 10, 0,25, 0,50 and 1,00 ppm for 10-minute dipping. In the cuttings allowed to grow and develop in a growth chamber were calculated firstly the ratios of sprouting, rooting and healthy plant. Later, in the rooted and sprouted cuttings, root development (root number, longest root length, root development scale, fresh and dry root weights, dry root ratio) and shoot development (primary shoot length, node number, shoot number, auxiliary shoot number) were determined. 140 Ru had always better growth and development compared to 41 B. Although IBA applications did not have distinctly significant effects on root and shoot development of the two rootstocks, applications of 5 second 1500 ppm IBA and 10 minute 1.00 ppm HBR were shown to stimulate rooting and healthy plant ratios in the rootstock 41 B. EBR, on the other hand, did not yield any success. Along with 1000 ppm IBA, 0.50 and 1.00 ppm HBR, all EBR treatments resulted in noticeable increase in the ratios of rooting and healthy plant in 140 Ru. This research showed that brassinosteroids might be used successfully as auxins in inducing rooting in grapevine rootstocks cuttings.

Keywords:

auxin, IBA, brassinosteroid, homobrassinolide, epibrassinolide, grapevine, rooting,

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