PUTRESCINE AS A PROTECTIVE MOLECULE ON DNA DAMAGE AND DNA METHYLATION CHANGES IN WHEAT UNDER DROUGHT

Öz The world suffers with the agricultural drought stress which leading to decreasing crop production, and also adversely affecting cereals on morphological, physiological, biochemical and molecular levels. However, exogenous treatment of some osmotically active materials like putrescine has been regarded as a good preventive against these harmful effects of drought. But there is a lack of information on putrescine has any effects on DNA damage and DNA methylation in crops. The current study was goal to determine DNA damage levels and DNA methylation changes in Triticum aestivum cv. Karasu 90 subjected to different concentrations of drought (-2, -4, -6 bar PEG) and whether putrescine (0.01, 0.1, 1 mM) has any ameliorative effect on these changes is determined with RAPDs and CRED-RAs tecniques. In addition, total oxidant status (TOS) and total antioxidant status (TAS) values were investigated based on drought and putrescine treatments. The findings showed that drought stress caused DNA damage and DNA methylation changes. However, these effects decreased after putrescine treatments. Putrescine has been shown to decrease oxidative damage caused by drought via increasing antioxidant status in drought stress. According to results, it was concluded that putrescine could be preferred for its force to protect wheat DNA from the damaging effects of drought and the demethylation positively contributed to drought stress tolerance.

Anahtar Kelimeler:

CRED-RA, DNA Methylation, Genomic Template Stability, RAPD, Total oxidant status, Total antioxidant status

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Alzahrani Y, Kuşvuran, A, Alharby HF, Kuşvuran S, Rady, MM (2018). The defensive role of silicon in wheat against stress conditions induced bydrought, salinity or cadmium. Ecotoxicology and Environmental Safety 154: 187-196.

Gill SS, Tuteja N (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48: 909-930.

Dutta S, Mitra M, Agarwal P, Mahapatra K, De Sayanti Sett U, Roy S (2018). Oxidative and genotoxic damages in plants in response to heavy metal stress and maintenance of genome stability. Plant Signaling and Behaviour 13: 1-17.

Gratão PL, Polle A, Lea PJ, Azevedo RA (2005). Making the life of heavy metal-stressed plants a little easier. Functional Plant Biology 32: 481-494.

Grativol C, Hemerly AS, Ferreira PCG (2012). Genetic and epigenetic regulation of stress responses in natural plant populations. Biochimica et Biophysica Acta-Gene Regulatory Mechanisms 1819: 176-185.

Kumar M, Bijo AJ, Baghel RS, Reddy CRK, Bhavanath J (2012). Selenium and spermine alleviate cadmium induced toxicity in the red seaweed Gracilaria dura by regulating antioxidants and DNA methylation. Plant Physiology and Biochemistry 51: 129-138.

Tan MP (2010). Analysis of DNA methylation of maize in response to osmotic and salt stress based on methylation-sensitive amplified polymorphism. Plant Physiology and Biochemistry 48: 21-26.

Kaur A, Grewal A, Sharma P (2018). Comparative analysis of DNA methylation changes in two contrasting wheat genotypes under water deficit. Biologia Plantarum 62: 471-478.

Labra M., Ghiani, A., Citterio, S., Sgorbati, S., Sala, F., Vannini, C., Ruffini-Castiglione, M. and Bracale M (2002). Analysis of cytosine methylaion pattern in response to water deficit in pea root tips, Plant Biology, 4, 694-699.

Wang WS, Pan YJ, Zhao XQ, Dwivedi D, Zhu LH, Ali J, Fu BY, Li ZK (2011). Drought-induced site-specific DNA methylation and its association with drought tolerance in rice (Oryza sativa L.). Journal of Experimental Botany 62: 1951-1960.

Devi EL, Kumar S, Singh TB, Sharma SK, Beemrote A, Devi CP, Prakash N (2017). Adaptation strategies and defence mechanisms of plants during environmental stress. In Medicinal Plants and Environmental Challenges. Springer, pp.359-413.

Upreti KK, Sharma M (2016). Role of plant growth regulators in abiotic stress tolerance. Rao NKS et al. (ed), Abiotic stress physiology of horticultural crops. Springer, pp.19-46.

Liu JH, Nakajima I, Moriguchi T (2011). Effects of salt and osmotic stresses on free polyamine content and expression of polyamine biosynthetic genes in Vitis vinifera. Biologia Plantarum 55: 340-344.

Erdal S, Aydin M, Genisel M, Taspinar MS, Dumlupinar R, Kaya O, Gorcek Z (2011). Effects of salicylic acid on wheat salt sensitivity. African Journal of Biotechnology 10: 5713-5718.

Galston AN, Kaur-Sawhney R, Altabella T, Tiburico AF (1997). Plant polyamines in reproductive activity and response to abiotic stress. Botanica Acta 110: 197–207.

Kumar A, Altabella T, Taylor MA, Tiburcio AF (1997). Recent advances in polyamine research. Trends in Plant Science 2: 124-130.

Miyamoto S, Kashiwagi K, Watanabe S, Igarashi K (1993). Estimation of polyamine distribution and polyamine stimulation of protein synthesis in Escherichia coli. Archieves of Biochemistry and Biophyics 300: 63-68.

Ruiz-Herrera J, Ruiz-Medrano R, Dominguez A (1995). Selective inhibition of cytosine-DNA methylases by polyamines. FEBS Letters 357: 192-196.

Michel BE, Kaufmann MR (1973). The osmotic potential of polyethyline glycol 6000. Plant Physiology 51: 914-916.

Taspinar MS, Aydin M, Arslan E, Yaprak M, Agar G (2017). 5-Aminolevulinic acid improves DNA damage and DNA Methylation changes in deltamethrin-exposed Phaseolus vulgaris seedlings. Plant Physiology and Biochemistry 118: 267-273.

Botstein D, White RL, Skolnick M, Davis RW (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics 32: 314-331.

Prevost A, Wilkinson MJ (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics 98: 107-112.

Peterson A, Bartish IV, Peterson J (2002). Genetic structure detected in a small population of the endangered plant Anthericum liliago (Anthericaceae) by RAPD analysis Ecography 25: 677-684.

Liu W, Yang YS, Zhou Q, Xie L, Li P, Sun, T (2007). Impact assessment of cadmium contamination on rice (Oryza sativa L.) seedlings at molecular and population levels using multiple biomarkers. Chemosphere 67: 1155-1163.

Taspinar MS, Agar G, Yildirim N, Sunar S, Aksakal O, Bozari S (2009). Evaluation of selenium effect on cadmium genotoxicity in Vicia faba using RAPD. Journal of Food, Agriculture and Environment 7: 857-860.

Cenkci S, Cigerci IH, Yildiz M, Ozay C, Bozdag A, Terzi H (2010). Lead contamination reduces chlorophyll biosynthesis and genomic template stability in Brassica rapa L.. Environmental and Experimantal Botany 67: 467-473.

Cenkci S, Yildiz M, Ciğerci I, Bozdag A, Terzi H, Terzi ESA (2010). Evaluation of 2,4-D and dicamba genotoxicity in bean seedlings using comet and RAPD assays. Ecotoxicology and Environmental Safety 73: 1558-1563.

Jamil A, Riaz S, Ashraf M, Foolad MR (2012). Gene expression profiling of plants under salt stress. Critical Reviews in Plant Sciences 30: 435-458.

Bano A, Ullah F, Nosheen A (2012). Role of abscisic acid and drought stress on the activities of antioxidant enzymes in wheat. Plant Soil and Environment 58: 181-185.

Suzuki N, Koussevitzky S, Mittler R, Miller G (2012). ROS and redox signalling in the response of plants to abiotic stress. Plant Cell and Environment 35: 259-270.

Perez-Amador MA, Leon J, Greem PJ, Carbonell J (2002). Induction of the arginine decarboxylase ADC2 gene provides evidence for the involvement of polyamines in the wound response in Arabidopsis. Plant Physiology 130: 1454-1463.

Capell T, Bassie L, Christou P (2004). Modulation of the polyamine biosynthetic pathway in transgenic rice confers tolerance to drought stress. Proceedings of the National Academy of Sciences of the United States of America 10: 9909-9914.

Kuznetsov VIV, Radyukina NL, Shevyakova NI (2006). Polyamines and stress: biological role, metabolism and regulation Russian Journal of Plant Physiology 53: 658-683.

Shi H, Ye T, Chan Z (2013). Comparative proteomic and physiological analyses reveal the protective effect of exogenous polyamines in the bermuda grass (Cynodon dactylon) response to salt and drought stresses. Journal of Proteome Research 12: 4951-4964.

Shi H, Chan Z (2014). Improvement of plant abiotic stress tolerance through modulation of the polyamine pathway. Journal of Integrative Plant Biology 56: 114-121.

Kim YH, Kim MD, Choi YI, Park SC, Yun DJ, Noh EW, Lee HS, Kwak SS (2011). Transgenic poplar expressing Arabidopsis NDPK2 enhances growth as well as oxidative stress tolerance. Plant Biotechnology Journal 9: 334-347.

Lu G, Wu X, Chen B, Gao G, Xu K (2007). Evaluation of genetic and epigenetic modification in Rapeseed (Brassica napus) induced by salt stress. Journal of Integrative Plant Biology 49: 1599-1607.

Zhao Y, Yu S, Ye W, Wang H, Wang J, Fang B (2010). Study on DNA cytosine methylation of cotton (Gossypium hirsutum L.) genome and its implication for salt tolerance. Agricultural Sciences in China 9: 783-791.

Steward N, Ito M, Yamaguchi Y, Koizumi N, Sano H (2002). Periodic DNA methylation in maize nucleosomes and demethylation by enviromental stress. The Journal of Biological Chemistry 277: 37741-37746.

Aina R, Sgorbati S, Santagostino A, Labra A, Ghiani A, Citterio S (2004). Specific hypomethylation of DNA is induced by heavy metals in white clover and industrial hemp. Plant Physiology 12: 472-480.

Labra M, Grassi F, Imazio S, Di Fabio T, Citterio S, Sgorbati S, Agradi E (2004). Genetic and DNA-methylation changes induced by potassium dichromate in Brassica napus L.. Chemosphere 54: 1049-1058.

Ruiz-Herrera J (1994). Polyamines, DNA methylation, and fungal differentiation. Critical Reviews in Microbiology 20: 143-150.

Valledor L, Hasbun R, Meijon M, Rodrı´guez JL, Santamarı´a E, Viejo M, Berdasco M, Feito I, Fraga M, Can˜al MJ, Rodrı´guez R (2007). Involvement of DNA methylation in tree development and micropropagation. Plant Cell Tissue and Organ Culture 91: 75-86.

Noceda C, Salaj T, Perez M, Viejo M, Canal MJ, Salaj J, Rodriguez R (2009). DNA demethylation and decrease on free polyamines is associated with the embryogenic capacity of Pinus nigra Arn. cell culture. Trees 23: 1285-1293.

Minocha R, Minocha SC, Long S (2004). Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce (Picea rubens Sarg.). In Vitro Cellular and Developmental Biology 40: 572-580.

Wada Y, Miyamoto K, Kusano H, Sano H (2004). Association between up-regulation of stress-responsive genes and hypomethylation of genomic DNA in to tobacco plants. Molecular Genetics and Genomics 271: 658-666.