INVESTIGATION OF THE ANTIMICROBIAL ACTIVITY OF Vitis vinifera L. BOĞAZKERE

Öz In this study, extracts from Boğazkere cultivar of Vitis vinifera L. at 10 µL, 50 µL and 100 µL concentrations were tested against 18 different bacterial and fungi strains using disc diffusion (DD) method along with minimum inhibitory concentration (MIC) and minimum bactericidal/fungisidal concentration (MBC/MFC) tests to reveal possible antimicrobial properties. Then, the obtained results were compared with 18 known antibiotics. The results revealed that 7.33-19.66 mm inhibition zones were obtained for 15-different microorganisms at 100 µL concentrations while those obtained 7.33-12.33 mm inhibition zones for 12 microorganims at 50 µL volume, where no inhibition zone was observed at 10 µL volume addition. The extracts of Boğazkere for the tested concentrations showed no antimicrobial capability against Salmonella kentucky, Enterococcus durans, Salmonella typhimurium and Candida ablicans. MIC tests showed that the extract at 0.039-20 mg/100 mL concentration range was bacteriostatic for the entire tested microorganism. Bactericidal effects of the extract were obtained for Listeria innocua at 10 mg /100 mL while that was 20 mg /100 mL for Pseudomonas fluorescens, Pseudomonas aeruginosa, Enterococcus faecium and Staphylococcus aureus. The findings show that Boğazkere grape species has antibiotic character, what makes them possible preservatives for food products.

Anahtar Kelimeler:

Antimicrobial activity, Disc diffusion method, Vitaceae, Vitis vinifera, Boğazkere

PDF

___

Aslankoç R, Demirci D, İnan Ü, Yıldız M, Öztürk A, Çetin M, Savran EŞ and Yılmaz B (2019). The Role Of Antioxidant Enzymes İn Oxidative Stress - Superoxide Dismutase (Sod), Catalase (Cat) And Glutathione Peroxidase (Gpx). Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi 26(3): 362-369.

Cheeseman KH and Slater TF (1993). An introduction to free radical biochemistry. British Medical Bulletin 49: 481-493.

Kohanski MA, Dwyer DJ and Collins JJ (2010). How antibiotics kill bacteria: from targets to networks. Nature Reviews Microbiology 8(6): 423–435.

Baydar NG, Sağdıç O, Özkan G and Çetin ES (2006). Determination of antibacterial effects and total phenolic contents of grape (Vitis vinifera L.) seed extracts. International Journal of Food Science and Technology 41: 799-804.

GTB (2015). 2014 Yılı Çekirdeksiz Kuru Üzüm Raporu, T.C. Gümrük ve Ticaret Bakanlığı Kooperatifçilik Genel Müdürlüğü, Şubat 2015. http://koop.gtb.gov.tr/data/53319ea4487c8eb1e43d72a0/2014%20Kuru%20%C3%9Cz%C3%BCm%20Raporu.pdf/. Access date: 03 .11.2015

Ateş F and Karabat S. (2016). Manisa Bağcılık Araştırma Enstitüsü Müdürlüğü, Sofralık Üzüm Yetiştirmeye Yönelik Kültürel Uygulamalar. [online]http://arastirma.tarim.gov.tr/manisabagcilik/Belgeler/genelbagcilik/Kalıtelı%20sofralık%20uzum%20yetıstırıcılıgı%20fadıme%20ates.pdf/. Access date: 11.10. 2016

Monagas M, Bartolome B and Gomez-Cordoves C (2005). Updated knowledge about the presence of phenolic compounds in wine. Critical Reviews in Food Science and Nutrition 45(2): 85-118.

Jayaprakasha G, Selvi T and Sakariah K (2003). Antibacterial and antioxidant activities of grape Vitis vinifera seed extracts. Food Research International 36(2): 117-122.

Baydar NG, Özkan G and Sağdiç O (2004). Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 15(5): 335-339

Anastasiadi M, Chorianopoulos NG, Nychas GJE and Haroutounian S (2009). Antilisterial activities of polyphenol-rich extracts of grapes and vinification by products. Journal of Agricultural and Food Chemistry 57(2): 457-463.

Ege D (2015). Bazı Zararlı Mikroorganizmaların Kültür Ortamindaki Gelişimine Üzüm (Vitis Vinifera L.) Çekirdeği Yağinin Etkileri. Yüksek lisans tezi, Selçuk Üniversitesi Fen Bilimleri Enstitüsü, Konya.

Abtahi H, Ghazavi A and Karimi M (2011). Antimicrobial activities of ethanol extract of black grape. African Journal of Microbiology Research 5(25): 4446-4448

Waqar A, Han Aİ, Waqar M, Han MA, Han A, Ramazan R, Wali S, Ahmad F, Han N, Yousaf S, Zeb M, Han EU, Rahman MU and Faysal Ş (2014). In vitro Antibacterial Activity of Vitis vinifera Leaf Extracts Against Some Pathogenic Bacterial Strains. Advances in Biological Research 8 (2): 62-67.

Yadav D, Kumar A, Kumar P and Mishra D (2015). Antimicrobial properties of black grape (Vitis vinifera L.) peel extracts against antibiotic-resistant pathogenic bakteri and toxin producing molds. Indian Journal of Pharmacology 47(6): 663-667.

Altuner EM, Çeter T and Işlek C (2010). Cilt enfeksiyonlarının geleneksel tedavisinde kullanılan Ononis spinosa L. külünün antifungal etkisinin araştırılması. Mikrobiyoloji Bülteni 44(4): 633-639

Altuner EM, Çeter T, Bayar E, Aydın S, Arıcı F, Süleymanoğlu G, Edis A (2011). Investigation on Antimicrobial Effects of Some Moss Species Collected From Kastamonu Region, Commun. Fac. Sci. Univ. Ank. Series C 23(1-2): 33-43

Altuner EM, Çeter T, Demirkapı D, Özkay K, Hayal U, Eser G (2011) Investigation on Antimicrobial Effects of Some Lichen Species Collected From Kastamonu Region, Commun. Fac. Sci. Univ. Ank. Series C 23(1-2): 21-31

Bauer AW, Kirby WMM, Sherris JC and Turck M (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clininical Pathology 36: 493-496.

Bader BMA, Çeter T and Bani B (2018). Investigation of Antimicrobial Activity of Gagea dubia. Commun. Fac. Sci. Univ. Ank. Series C; Biology 27(2): 224-231

Altuner EM, Çeter T, Gür M, Güney K, Kıran B, Akwieten HE, Soulman SI (2018). Chemical Composition and Antimicrobial Activities of Cold-Pressed Oils Obtained From Nettle, Radish and Pomegranate Seeds. Kastamonu Üniversitesi Orman Fakültesi Dergisi 18(3): 236 - 247