Cryptococcus neoformans kökenlerinin in vitro fenol oksidaz etkinliğinin belirlenmesi ve C. neoformans'ın Candida albicans kolonilerinden ayırt edilmesi için Pal agar ve Niger seed agar besiyerlerinin karşılaştırılması

Helianthus annus (ayçiçeği) tohumundan hazırlanması dolayısıyla kafeik asit içeren Pal agarı (PA) Cryplococcus neoformans kökenlerinin fenoloksidaz etkinliğini belirlemek ve ilk kültür besi veri olarak C neoformans kolonilerini renk farkı ile Candida albicans'ınkilerden ayırt etmek için değerlendirildi, Her deneyde Pal ağarın yanısıra Niger seed agarı (NSA) kullanıldı. Deneylerde çevre ve klinik kaynaklı 37 ve bir referans C. neoformans kökeni ile I C. neoformans ve 8 C. albicans referans kökeni kullanıldı. Hem PA hem de NSA C. neoformans'in fenoloksidaz etkinliğini belirlemek ve C. neoformans kolonilerini C. albicans'ınkilerden ayırt etmek için güvenilir sonuç verdiler. PA'nın ayrıca, ucuz ve kolay bulunabilir tohumlardan hazırlanması dolayısıyla rutin laboratuar koşullarında kullanıma daha elverişli olduğu belirlendi. Bu besiyerinin mikrobiyoloji laboratuarlarında rutin kullanılması, başta HIV ile infekte hastaların ağız ve trakea sekresyonlarından, ayrıca habis tümörlüler, divabetliler ve hatta mantarın doğadaki kaynağı ile temas etmiş sağlam görünümlü olanlar da dahil geniş bir hasta grubundan C. neoformans'ın kolay, erken ve hızlı tanımına yardımcı olabileceği sonucuna varıldı.

Comparison of Pal's agar with Niger seed agar to to detect in vitro phenoloxidase activity of Cryptococcus neoformans strains and to differentiate C. neoformans from Candida albicans colonies

Pal's agar (PA) which contains caffeic acid due to I Helianthus annus (sunflower) was investigated to detect phenoloxidase activity of Crvptococcus neoformans strains and to differerenliate C. neoformans colonies from those of Candida albicans with color differences as a primary culture medium. Niger seed agar ıNSAı was used along with PA in eac h lest, Fifty seven clinical and environmental C. neoformans isolates plus 1 C. neoformans and 8 C. albicans reference strains were used for the tests. Both PA and NSA medium showed reliable results in the detection of phenoloxidase activity of C. neoformans and ihe differentiation of C. neoformans colonies from C. albicans colonies. PA is also easy and inexpensive If prepare from available seeds in routine laboratory conditions. Its routine use in microbiology laboratories rould be helpful for easv, early and rapid diagnosis of C. neoformans infection from a wide range of patient population, particularly from HIV infected patients' oral and iracheal secretion samples as well as from other at risk patients such as malignarHs, diabetics or even healthy individuals who possiblv get in contact with natural sources of the fungus.

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