Sibel AKYOL, Özdemir İLTER, Bayram KIRAN, Halil TUNALI

Alkolik yapılan gebe sıçanlar ve yavrularında NK aktivasyonu ile IL-2, IFN-gama ve CD19 etkileşimi

Çalışmamız NK hücrelerinin, alkolik yapılan gebe sıçanlar ve yavrularında IL-2 , IFN-y ve CD 19 ile nasıl etkileşim içinde olduğunu göstermek amacıyla planlandı. Toplam 80 Wistar albino soyu dişi sıçanda yapılan araştırmanın grupları; 1) Kontrol Grubu (K) (n=10) 2) Gavajla eta-nol uygulanan grup (G) (n=10) 3) Kontrol gebe grubu (KG) (n=10) 4) Gavajla etanol uygulanan gebe grubu (GG) (n=10) 5) Kontrol 10 günlük yavru (KY10) (n=10) 6) Kontrol 30 günlük yavru (KY30 ) (n=10) 7) Gavajla etanol uygulanan gebenin 10 günlük yavrusu (GY10) (n=10) 8) Gavajla etanol uygulanan gebenin 30 günlük yavrusu (GY30) (n=10) şeklinde belirlendi. NK değerlerinin [(K: %54.90 ± 10.86 ile G: % 38.40 ± 3.43), (KG: %40.00±2.1 ile GG:%37.1±2.10 )] grupları arasında, 10 ve 30 günlük yavru [(KY10: % 30.2± 2.1 üe GY10: %13.0 ± 0.7), (KY30: %32.28 ± 2.6 ile GY 30: % 20.75 ± 1.2)] grupları arasında anlamlı düşüş olduğu belirlendi. IL-2 değerlerinin [(K: 86.5 ± 1.3 pg/ml ile G:71.0±2.4 pg/ml.) (KG: 65.9 ±1.1 pg/ml ile G.G: 60.9 ±2.1 pg/ml.)] grupları arasında, 10 ve 30 günlük yavru [( KY10: 75.4 ± 3.2 pg/ml ile GY10: 63.0 ± 3.2 pg/ml ), (KY30: 76.0 ± 3.4 pg/ml ile GY30: 70.0 ± 2.6 pg/ml)] grupları arasında anlamlı dü-şüş olduğu görüldü. IFN-y değerlerinin [(K: 1250 ± 29.6 pg/ml ile G: 860 ±27.3 pg/ml ), (KG: 720$pm$13.6 pg/ml ile GG: 570 ± 9.1 pg/ml )] grupları arasında, 10 ve 30 günlük yavru [(KY10: 850 ± 25.0 pg/ml ile GY10: 520 ± 17pg/ml ) (KY30: 900 ± 10 pg/ml ile GY30: 640 ± 16.10 pg/ml )] grupları arasında anlamlı azalma gözlendi. CD19 değerleri [(K: %25.73 ± 3.07 ile G: %23.83 ± 1.6) grupları arasında anlamlılık belirlenemedi. (KG: %23.98 ± 1.7 ile GG: %18.46 ± 1.7) grupları arasında anlamlı düşüş görüldü. 10 ve 30 günlük yavru [(KY10: %11.15 ± 1.7 ile GY10: %32.5 ± 2.0 ) (KY30: %21.35 ± 1.2 ile GY30: %30.8 ± 1.8)] grupları arasında anlamlı artış belirlendi. Gavaj yöntemiyle alkol uygulanan dişi sıçanlarda ve bunların gebelik döneminde kontrol grubuna göre NK aktivitesinin anlamlı olarak azaldığı, buna paralel olarak CD-19, IL-2 ve IFN-y düzeyinde de azalma olduğu görüldü. Gebelik döneminde alınan alkolün, 10 günlük yavrularda NK, IL-2, IFN-y düzeyinde, 30 günlük yavrulara göre daha güçlü supresif etkisi olduğu belirlendi. Gerek 10 gerekse 30 günlük yavrularda CD 19 düzeyinde anlamlı artışın olduğu gözlendi. Gebelik sürecinde annede gelişen özel immün tolerans sistemlerinin teratojenik faktörlerin başında gelen alkol ile değişime uğradığı sonucuna varıldı.

The NK activation and the interaction of the parameters IL-2, IFN-gamma and CD19 in the alcoholic pregnant rats and their offspring

Background.- Our study aims to show how the NK cells interact with the parameters of IL-2, IFN-y and CD 19 in the alcoholic pregnant rats and their offspring. Several experimental animal models have been described for the study that was carried out on a total number of 80 Wistar albino female rats. Design.- In our study, rats were divided into 8 groups; 1) Control group (K) (n=10) 2) Group that received ethanol through gavage (G) (n=10) 3) Control pregnant group (KG) (n=10) 4) Pregnant group that received ethanol through gavage (GG) (n=10) 5) Control offspring (10 days old) (KY10) (n=10) 6) Control offspring (30 days old) (KY30) (n=10) 7) 10-day-old offspring of pregnant rats that received ethanol through gavage (GY10) (n=10) 8) 30 day old off-spring of pregnant rats that received ethanol through gavage (GY30) (n=10). Results.- Data from this study show that there has been a considerable decrease in the NK values the successive groups [(K: % 54.90±10.86 and G: % 38.40±3.43), (KG: % 40.00±2.1 and GG: % 37.1 ±2.10)] and the groups of 10 to 30 day old offspring of rats [(KY10: %30.2±2.1 and GY10: %13.0±0.7), (KY30: %32.28±2.6 and GY30: %20.75± 1.2)] respectively. A significant decrease is observed in the IL-2 values between the consecutive groups [(K: 86.5±1.3 pg/ml and G: 71.0±2.4 pg/ml), (KG: 65.9±1.1 pg/ml and GG: 60.9±2.1 pg/ml )] and the groups of 10 to 30 day old offspring of rats [(KY10: 75.4±3.2 pg/ml and GY10: 63.0±3.2 pg/ml), (KY30: 76.0±3.4 pg/ml and GY30: 70.0±2.6 pg/ml)] respectively. The IFN-y values indicated a considerable decrease among the successive groups [(K: 1250±29.6 pg/ml and G: 860±27.3 pg/ml) (KG: 720±13.6 pg/ml and GG: 570±9.1 pg/ ml)] and the groups of 10 to 30 day old off-spring of rats [(KY10: 850±25.0 pg/ml and GY10: 520±17 pg/ml) (KY30: 900±10 pg/ml and GY30: 640±16.10 pg/ml) ] respectively. No significant changes were observed in the CD-19 values between the groups [(K: % 25.73±3.07 and G: % 23.83H.6) while there was a significant decrease between the groups (KG: %23.98±1.7 and GG: % 18,46± 1.7)] The CD-19 values displayed a considerable increase among the consecutive groups of [(KY10: %11.15±1.7 and GY10: % 32.5$pm$2.0) (KY30: %21.35±1.2 and GY30: % 30.8±1.8)] respectively. Conclusions.- It has been observed that in the female pregnant rats that received alcohol through gavage, the NK activity falls considerably according to the control group and in parallel to that there is a decrease in the levels of the parameters CD 19, IL-2 and IFN-y. Compared to the 30-day old-offspring, a more suppressive effect was observed in the NK, IL-2 and IFN-y levels of the 10 day old offspring of the rats that received alcohol during the period of pregnancy. Also in the CD-19 level, a significant increase is observed both in 10 and 30 day old offspring. These results point out the fact that the teratogenic factors like alcohol are primarily responsible for the profound and harmful effects on the special immune tolerance systems that have developed through the period of pregnancy of the mother.

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