Züleyha ERİŞGİN, Şeyma KURT, Yavuz TEKELİOĞLU, Ahmet Uğur AKMAN, Sibel TÜREDİ

Protective Effects of Vitamin E against Methotrexate-Induced Testicular Damage in Rats: Histopathologic and flow cytometric study

Objective: Methotrexate (MTX) can cause oxidative stress-related tissue damage.Vitamin E neutralizes lipid peroxidation arising from the effect of free oxygen radicals.In this study, the protective effect of vitamin E against possible MTX-related testicular damage was analyzed. Materials and Methods: Thirty two mature male Spraque dawley rats were grouped as MTX, Vitamin E, MTX+vitamin E and control groups. 20 mg/kg MTX intraperitoneal (i.p.) was applied to MTX Group in the first day; 100mg/kg i.p. vitamin E was applied to vitamin E Group for 5 days; 20 mg/kg i.p. MTX in the first day and 100 mg/kg i.p. vitamin E for 5 days were applied to MTX+vitamin E Group;2 ml physiological saline solution (i.p.) was applied to control group for 5 days. Histopathology and flow cytometric analysis were done and apoptosis was evaluated on testicular tissue. Results: Apoptotic Index (%) and testicular damage were highest for MTX Group, and significant decrease was observed for MTX+vitamin E Group compared to MTX Group.Seminiferous tubule size significant decreased in MTX Group and it increased in MTX+vitamin E Group compared to MTX Group. No significant difference was found between MTX and MTX+vitamin E Groups regarding germinal epithelium thickness and testicle weights. Conclusion: The results show that MTX can cause structural disruptions in testicles and vitamin E can rehabilitate MTX-related testicular damage

Sıçanlarda Metotreksat ile İndüklenen Testis Hasarına Karşı E Vitamininin Koruyucu Etkileri: Histopatolojik ve Akım Sitometrik Çalışma

Giriş ve Amaç: Metotreksat oksidatif strese bağlı doku hasarına neden olmaktadır. E vitamini serbest oksijenradikallerinin etkisiyle oluşan lipit peroksidasyonunu nötralize eder. Bu çalışma ile E vitamininin olası metotreksatbağlantılı testiküler hasara karşı koruyucu etkileri analiz edildi.Gereç ve Yöntemler: Otuz iki erkek Spraque dawley sıçan; metotreksat (MTX), E vitamini, MTX+ E vitamini vekontrol olarak gruplandırıldı. İlk gün MTX grubuna intraperitoneal(i.p.) 20 mg/kg MTX, E vitamini grubuna 5 gün100 mg/kg i.p. E vitamini, MTX+E vitamini grubuna ilk gün 20 mg/kg i.p. MTX ve 5 gün 100 mg/kg i.p. E vitaminiuygulandı. Kontrol grubuna 5 gün 2 ml serum fizyolojik (i.p.) uygulandı. Testiküler dokuda histopatolojik, akımsitometrik analizler yapıldı ve apopitoz değerlendirildi.Bulgular: Apopitotik indeks (%) ve testiküler hasar en fazla MTX grubundayken, MTX+E vitamini grubunda MTXgrubuna göre anlamlı derecede azalma gözlendi. Seminifer tübül çapı MTX grubunda belirgin şekilde azalırken,MTX+E vitamini grubunda MTX grubuna göre artış gözlendi. MTX ve MTX+E vitamini grupları arasında germinalepitelyum ve testiküler ağırlık sonuçları açısında anlamlı farklılık yoktu.Sonuç: Sonuçlara göre MTX testislerde yapısal hasara neden olabilirken, E vitamini MTX'e bağlı testiküler hasarıdüzeltebilir

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