Tokat Bölgesinde Yetişen Bazı Yabani Mantar Türlerinin Antioksidan Aktivitelerinin Belirlenmesi

Yabani yenilebilir mantarlar sağlık için yenebilir ve içerdiği çeşitli biyoaktif bileşenler nedeniyle sinerji yaratarak sağlıklı bir yaşam sürdürmede önemli bir rol oynayabilir. Bununla birlikte, biyoaktif bileşikler içerebilen birçok tür araştırılmamıştır. Bu çalışmanın amacı Tokat bölgesinde yetişen Leatiporus sulpherus, Ramaria sp., Cantharellus aerruginascens, Verpa conica, Verpa bohamica, Tricholoma terreum, Agaricus sp., Helvella elastica isimli doğal mantar türlerinin antioksidan kapasitesini araştırmaktır. Ayrıca, mantar örneklerinde total fenolik bileşik ve E vitamini seviyeleri analiz edilmiştir. Mantar örneklerinin antioksidan kapasitesini belirlemek için serbest radikal giderme aktivitesi (DPPH), indirme gücü tayini, ABTS • + [2,2´-Azino-bis (3-etilbenzotiyazolin-6-sülfonik asit)] katyon radikali giderme aktivite testleri yapılmıştır. Sonuçlar standart olarak kullanılan Bütillenmiş hidroksi toluen (BHT), Bütillenmiş hidroksi anisol (BHA) ve α-tokoferol konsantrasyonları ile karşılaştırıldı. Radikal giderme aktivitesi en yüksek olan mantar türlerinin sırasıyla Ramaria sp., V. bohamica ve H. elastica olduğu, E vitamini yüksek olan mantar türlerinin ise Agaricus sp. (1444.1 mg/kg), C. aerruginascens (1370.8 mg/kg) ve Ramaria sp. (1204.2 mg/kg) olduğu belirlendi. Total fenolik bileşik miktarının en yüksek olduğu türün Ramaria sp. (6.57 g/kg) olduğu tespit edildi. Sonuç olarak bu çalışma, seçilen mantar türlerinin beslenme ve tıbbi özelliklerini değerlendirmek için daha fazla çalışmaya teşvik edebilir.

Determination of Antioxidant Activities of Some Wild Mushrooms Species in Tokat Region

Wild edible mushrooms can be eaten for health and play an important role in maintaining a healthy life by creating synergy due to the various bioactive components it contains. However, many species that may contain bioactive compounds have not been investigated.The purpose of this study was to investigate the antioxidant capacity of wild mushroom species which are grown in Tokat region with names Leatiporus sulpherus, Ramaria sp., Cantharellus aerruginascens, Verpa conica, Verpa bohamica, Tricholoma terreum, Agaricus sp., Helvella elastica in vitro. In addition, total phenolic compound and vitamin E levels were analyzed in mushroom samples. In order to determine the antioxidant capacity of mushroom samples, free radical scavenging activity (DPPH), reducing power activity and ABTS•+ [2,2´-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)] cation radical scavenging activity analyzes were performed. The results were compared with concentrations of Butylated hydroxy toluene (BHT), Butylated hydroxy anisole (BHA) and α-tocopherol. Consequently, the mushroom species with the highest radical removal activity are Ramaria sp., V. bohamica and H. elastica, respectively. The highest value of vitamin E, was found in Agaricus sp. (1444.1 mg/kg), C. aerruginascens (1370.8 mg/kg) and Ramaria sp. (1204.2 mg/kg) respectively. The highest amount of total phenolic contains was found to be in the Ramaria sp. (6.57 g/kg). These results may encourage further studies to assess nutritional and medicinal properties of selected mushroom species.

___

  • Elmastaş, M., Gülçin, I., Işildak, Ö., Küfrevioǧlu, Ö. I., Ibaoǧlu, K., & Aboul-Enein, H. Y. (2006). Radical scavenging activity and antioxidant capacity of bay leaf extracts. Journal of the Iranian Chemical Society. https://doi.org/10.1007/BF03247217
  • Elsayed, E. A., El Enshasy, H., Wadaan, M. A. M., & Aziz, R. (2014). Mushrooms: A potential natural source of anti-inflammatory compounds for medical applications. Mediators of Inflammation. https://doi.org/10.1155/2014/805841
  • Friedman, M. (2016). Mushroom Polysaccharides: Chemistry and Antiobesity, Antidiabetes, Anticancer, and Antibiotic Properties in Cells, Rodents, and Humans. Foods. https://doi.org/10.3390/foods5040080
  • Gallego, P., Rojas, Á., Falcón, G., Carbonero, P., García-Lozano, M. R., Gil, A., … Del Campo, J. A. (2019). Water-soluble extracts from edible mushrooms (: Agaricus bisporus) as inhibitors of hepatitis C viral replication. Food and Function, 10(6), 3758–3767. https://doi.org/10.1039/c9fo00733d
  • Gülçin, I., Sat, I. G., Beydemir, S., & Küfrevioglu, Ö. I. (2004). Evaluation of the in vitro antioxidant properties of broccoli extracts (Brassica oleracea L.). Italian Journal of Food Science.
  • Gülçin, Ilhami. (2006). Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology. https://doi.org/10.1016/j.tox.2005.09.011
  • Gülçin, Ilhami. (2010). Antioxidant properties of resveratrol: A structure-activity insight. Innovative Food Science and Emerging Technologies. https://doi.org/10.1016/j.ifset.2009.07.002
  • Miller, N. J., & Rice-Evans, C. A. (1997). Factors influencing the antioxidant activity determined by the ABTS.+ radical cation assay. Free Radical Research. https://doi.org/10.3109/10715769709097799
  • Oktay, M., Gülçin, I., & Küfrevioǧlu, Ö. I. (2003). Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT - Food Science and Technology. https://doi.org/10.1016/S0023-6438(02)00226-8
  • Oyaizu, M. (1986). Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics. https://doi.org/10.5264/eiyogakuzashi.44.307
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. https://doi.org/10.1016/S0891-5849(98)00315-3
  • Singh, R. S., Walia, A. K., & Kennedy, J. F. (2019). Mushroom lectins in biomedical research and development. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2019.10.180
  • Slinkard, K., & Singleton, V. L. (1977). Total Phenol Analysis: Automation and Comparison with Manual Methods. American Journal of Enology and Viticulture, 28(1), 49–55.
  • Su, X., Liu, K., Xie, Y., Zhang, M., Wang, Y., Zhao, M., … Wang, J. (2019). Protective effect of a polyphenols-rich extract from Inonotus Sanghuang on bleomycin-induced acute lung injury in mice. Life Sciences, 230, 208–217. https://doi.org/10.1016/j.lfs.2019.05.074
  • Wu, D., Pae, M., Ren, Z., Guo, Z., Smith, D., & Meydani, S. N. (2007). Dietary Supplementation with White Button Mushroom Enhances Natural Killer Cell Activity in C57BL/6 Mice. The Journal of Nutrition, 137(6), 1472–1477. https://doi.org/10.1093/jn/137.6.1472