Objective:The aim of the study was to investigate serum folate, vitamin B12, homocysteine and vitamin D levelsof children with specific learning disorder, which is one of the most common psychiatric disorders in childhood period. Material and Methods: 42 children with learning disorders and 30 healthy controls were compared. Serum folate, vitamin B12, homocysteine and vitamin D levels were measured.Results: Folate levels were significantly lower in the patient group compare to the healthy controls (p = 0.046). There were no significant differences between the two groups in terms of vitamin B12 and homocysteine levels (p = 0.059 and p = 0.077 respectively). Vitamin D was significantly lower in the patient group (p <0.001).Conclusion: These results suggest that deficiency or insufficiency of vitamin D, folate and vitamin B12 may be present in children with specific learning disorder, which may contribute to the pathogenesis of the disorder. This study is important because it is the first study to investigate vitamin D, folate and homocysteine related to one carbon metabolism in children with learning disorders.