DİYAFRAMIN FARKLI PERİYODİK UYARIM YANITLARINDA ABDOMİNAL İSKEMİ- REPERFÜZYONUN YARATTIĞI DEĞİŞİMLER

Amaç: Abdominal aort anevrizması tedavisinin deneysel bir modeli olan abdominal iskemi-reperfüzyon kardiyolojik,solunumsal, renal ve üremeyle ilgili ikincil fonksiyon bozukluklarına neden olmaktadır. Yapmış olduğumuzçalışma ile ikincil bir komplikasyon olarak gelişen solunumsal fonksiyon bozukluğunun altında yatandiyafram kası mekanik özelliklerinde meydana gelen olası değişimlerin araştırılması amaçlanmıştır.Gereç ve Yöntemler: Bu amaçla gerçekleştirilen deneylerde 16 adet wistar albino türü sıçan kullanılmıştır. Abdominalaortanın infrarenal bölgesinden vasküler klip ile 30 dakika iskemi, klip kaldırılarak 2 saat reperfüzyongerçekleştirilen grup I/R olarak adlandırılırken, yalnızca insizyon gerçekleştirilen kontrol grubu ise KON olarakadlandırılmıştır. Sıçanların diyafram kasları frenik sinirleriyle birlikte izole edilerek in vitro kasılma ölçüm deneylerigerçekleştirildi. Uyarım 25 Hz sabit ve frekansı 25-50 Hz aralığında değişen supramaksimal uyaranlarlayapıldı.Bulgular: Tepe Değeri (TD, N), Alt Değer (AD, N), Gevşeme Miktarı (GM, N) verileri I/R grubunda anlamlı olarakazalmış bulunurken, Kasılma Süresinde (KS, ms) anlamlı (p

Changes in Different Periodic Stimulation Responses of Diaphragma

Objectives: Abdominal ischemia-reperfusion, which is an experimental model of abdominal aortic aneurysm treatment, causes secondary cardiological, respiratory, renal and reproductive dysfunctions. With this study, we aimed to investigate possible changes in the mechanical properties of the diaphragm underlying respiratory dysfunction. Materials and Methods: For this purpose, 16 wistar albino rats were used in the experiments. Ischemia was induced by using vascular clips from the infrarenal region of the abdominal aorta for 30 minutes. Then clip was removed for 2 hours reperfusion in I/R group. Only incision was made in control group which is named as KON group. Diaphragm muscles of rats were isolated together with phrenic nerves and in vitro contraction measurement experiments were performed. The stimulation was performed with 25 Hz constant and varying between 25-50 Hz supramaximal stimuli. Results: While Maximum Tension (MT, N), Basal Tension (BT, N), Relaxation (R, N) values decreased significantly in I/R group, Contraction Time (CT, ms) did not change significantly (p

PDF

___

1. Norwood MG, Bown MJ, Sayers RD. Ischaemia-reperfusion injury and regional inflammatory responses in abdominal aortic aneurysm repair. Eur J Vasc Endovasc Surg. 2004;28(3):234-45.
2. Sasaki M, Joh T. Oxidative stress and ischemia-reperfusion injury in gastrointestinal tract and antioxidant, protective agents. J Clin Biochem Nutr. 2007;40(1):1-12.
3. Volta CA, Ferri E, Marangoni E, Ragazzi R, Verri M, Alvisi V, Zardi S, Bertacchini S, Gritti G, Alvisi R. Respiratory function after aortic aneurysm repair: a comparison between retroperitoneal and transperitoneal approaches. Intensive Care Med. 2003;29(8):1258-64.
4. Sakalihasan N, Limet R, Defawe OD. Abdominal aortic aneurysm, Lancet. 2005;365:1577–89.
5. Katzen BT, MacLean AA. Complications of endovascular repair of abdominal aortic aneurysms: a review. Cardiovasc Intervent Radiol. 2006;29(6):935-46.
6. Bülbring E. Observations on the isolated phrenic nerve diaphragm preparation of the rat. Br J Pharmacol Chemother. 1946;1:38-61.
7. Close RI. Dynamic properties of mammalian skeletal muscles. Physiol Rev. 1972;52:129–97.
8. McLaughlin R, Kelly CJ, Kay E, Bouchier-Hayes D.Diaphragmatic dysfunction secondary to experimental lower torso ischaemiareperfusion injury is attenuated by thermal preconditioning. Br J Surg. 2000;87(2):201-5.
9. Moneley D, Barry MC, McLaughlin R, Kelly CJ, Bouchier Hayes DJ. Preoperative treatment with recombinant human growth hormone prevents ischemia reperfusion-induced diaphragmatic dysfunction. J Surg Res. 2001;97(1):81-4.
10. Ikebe K, Kato T, Yamaga M, Hirose J, Tsuchida T, Takagi K. Increased ischemia-reperfusion blood flow impairs the skeletal muscle contractile function. J Surg Res. 2001;99(1):1-6.
11. Reimer KA, Jennings RB, Tatum AH. Pathobiology of acute myocardial ischemia: metabolic, functional and ultrastructural studies. Am J Cardiol. 1983;52(2):72A-81A.
12. Herve P, Lecarpentier Y, Brenot F, Clergue M, Chemla D, Duroux P. Relaxation of the diaphragm muscle: influence of ryanodine and fatigue. J Appl Physiol. 1988;65(5):1950-6.
13. Zavecz JH, Anderson WM. Role of extracellular Ca2+ in diaphragmatic contraction: effects of ouabain, monensin, and ryanodine. J Appl Physiol. 1992;73(1):30-5.
14. Kong FJ, Berger AJ. Firing properties and hypercapnic responses of single phrenic motor axons in the rat. Appl Physiol. 1986;61(6):1999-2004.
15. Ionno M, Moyer M, Pollarine J, van Lunteren E. Inotropic effects of the K+ channel blocker 3,4-diaminopyridine on fatigued diaphragm muscle. Respir Physiol Neurobiol. 2008;160(1):45-53.
16. Van Lunteren E, Moyer M, Torres A. ATP-sensitive K+ channel blocker glibenclamide and diaphragm fatigue during normoxia and hypoxia. J Appl Physiol. 1998;85(2):601-8.
17. Bruton J. What does the membrane KATP channel really do in skeletal muscle? J Physiol 2010;588(22):4333.
18. Boudreault L, Cifelli C, Bourassa F, Scott K, Renaud JM. Fatigue preconditioning increases fatigue resistance in mouse flexor digitorum brevis muscles with non-functioning K(ATP) channels. J Physiol. 2010;588(Pt 22):4549-62.
19. Tuncer S, Dalkilic N, Burat I., Electrophysiological alterations in diaphragm muscle caused by abdominal ischemia-reperfusion. Respir Physiol Neurobiol. 2017 Apr;238:7-13.