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DİYAFRAMIN FARKLI PERİYODİK UYARIM YANITLARINDA ABDOMİNAL İSKEMİ- REPERFÜZYONUN YARATTIĞI DEĞİŞİMLER

Amaç: Abdominal aort anevrizması tedavisinin deneysel bir modeli olan abdominal iskemi-reperfüzyon kardiyolojik,solunumsal, renal ve üremeyle ilgili ikincil fonksiyon bozukluklarına neden olmaktadır. Yapmış olduğumuzçalışma ile ikincil bir komplikasyon olarak gelişen solunumsal fonksiyon bozukluğunun altında yatandiyafram kası mekanik özelliklerinde meydana gelen olası değişimlerin araştırılması amaçlanmıştır.Gereç ve Yöntemler: Bu amaçla gerçekleştirilen deneylerde 16 adet wistar albino türü sıçan kullanılmıştır. Abdominalaortanın infrarenal bölgesinden vasküler klip ile 30 dakika iskemi, klip kaldırılarak 2 saat reperfüzyongerçekleştirilen grup I/R olarak adlandırılırken, yalnızca insizyon gerçekleştirilen kontrol grubu ise KON olarakadlandırılmıştır. Sıçanların diyafram kasları frenik sinirleriyle birlikte izole edilerek in vitro kasılma ölçüm deneylerigerçekleştirildi. Uyarım 25 Hz sabit ve frekansı 25-50 Hz aralığında değişen supramaksimal uyaranlarlayapıldı.Bulgular: Tepe Değeri (TD, N), Alt Değer (AD, N), Gevşeme Miktarı (GM, N) verileri I/R grubunda anlamlı olarakazalmış bulunurken, Kasılma Süresinde (KS, ms) anlamlı (p

Changes in Different Periodic Stimulation Responses of Diaphragma

Objectives: Abdominal ischemia-reperfusion, which is an experimental model of abdominal aortic aneurysm treatment, causes secondary cardiological, respiratory, renal and reproductive dysfunctions. With this study, we aimed to investigate possible changes in the mechanical properties of the diaphragm underlying respiratory dysfunction. Materials and Methods: For this purpose, 16 wistar albino rats were used in the experiments. Ischemia was induced by using vascular clips from the infrarenal region of the abdominal aorta for 30 minutes. Then clip was removed for 2 hours reperfusion in I/R group. Only incision was made in control group which is named as KON group. Diaphragm muscles of rats were isolated together with phrenic nerves and in vitro contraction measurement experiments were performed. The stimulation was performed with 25 Hz constant and varying between 25-50 Hz supramaximal stimuli. Results: While Maximum Tension (MT, N), Basal Tension (BT, N), Relaxation (R, N) values decreased significantly in I/R group, Contraction Time (CT, ms) did not change significantly (p

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