2016

DERMAL FİBROBLAST HÜCRELERİNDE OLEUROPEİNİN ANTİOKSİDAN ÖZELLİĞİNİN VE YAŞLANMA ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI

Amaç: Yapılan çalışmada hidrojen peroksit ile oksidatif stres oluşturulmuş dermal fibroblast hücrelerine farklıdozlarda oleuropein uygulanmış ve oleuropeinin oksidatif strese ve yaşlanma mekanizmasına karşı etkileriincelenmiştir.Gereç ve yöntem: Oleuropeinin dermal fibroblast hücreleri üzerindeki toksik etkisi MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) analiziyle belirlenmiştir. Oksidatif stres oluşturulmuş dermal fibrob-last hücrelerinde oleuropeinin total antioksidan kapasite üzerine etkisi total antioksidan durumu inceleme kitive total oksidan kapasite üzerine etkisi, total oksidan durumu inceleme kiti ile belirlenmiştir. Senesensin (yaş-lanmanın) test edilmesinde standart protokollerde yer alan SA-ß-gal (senescence associated ß-galaktosidase)aktivitesi hücrelere verilen X-gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) substratının parça-lanması yöntemi ile ölçülmüştür.Bulgular: Oleuropeinin toksik doz düzeyi 750 μM olarak saptanmıştır. BJ Fibroblast hücrelerinde 24 saat süreile H2O2 ile inkübasyonundan kaynaklanan total oksidan durumunda istatistiksel olarak anlamlı olmayan birazalma gözlemlenmiştir (p>0,05). Oleuropein ile muamele edilen BJ Fibroblast hücre gruplarında 24 saat süreile H2O2 ile muamele edilen BJ Fibroblast hücre gruplarına göre total antioksidan durumunda istatistikselolarak anlamlı bir artış gözlemlenmiştir (p

The Investigation of the Antioxidant Characterization and the Effects on Aging of Oleuropeinine on the Dermal Fibroblast Cells

Aim: In the study, oleuropein was administered to dermal fibroblast cells oxidatively stressed with hydrogen peroxide at different doses and the effects of oleuropein on oxidative stress and aging mechanism were investigated. Materıal Method: The toxic effect of oleuropein on dermal fibroblast cells was determined by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) analysis. The effect of oleuropein on total antioxidant capacity in oxidative stressed dermal fibroblast cells was evaluated with total antioxidant status determination kit and the effect of oleuropein on total oxidant capacity in oxidative stressed dermal fibroblast cells was evaluated with total oxidant status determination kit. SA-ß-gal (senescence associated ß-galactosidase) activity, which is included in standard protocols when senescence is tested, was measured by the method of disruption of the X-gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) substrate given to the cells. Results: The toxic dose level of oleuropein was determined as 750 μM. A statistically insignificant reduction in the total oxidant status from incubation with H2O2 for 24 hours was observed in BJ Fibroblast cell line (p>0,05). A statistically significant increase in the total antioxidant status was observed in oleuropein treated BJ fibroblast cell groups compared to BJ Fibroblast cell groups treated with H2O2 for 24 hours (p

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