Mete ÖZKOÇ, Burçin Sim ÖZBAL, Ergül MUTLU ALTUNDAĞ

Sisplatin ve Timokinon Kombinasyonunun MCF-7 Hücrelerindeki Antiproliferatif Etkisinin Değerlendirilmesi

Meme kanseri, dünyada kadınlar arasında en sık en sık görülen ikinci ölüm nedeni ve en sık teşhis edilen kanser türüdür. Kemoterapötik bir ajan olan, sisplatin (CDPP), meme kanseri tedavisinde kullanılmakla birlikte ciddi yan etkilere sahiptir. Ayrıca, MCF-7 gibi meme kanseri hücrelerinin cisplatine karşı dirençli olması tedaviyi zorlaştırmaktadır. Çörek otunda bulunan ve fitokimyasal bir bileşik olan timokinon (TQ) farklı çalışmalarda antikanser ajan olarak uygulanmış ve araştırılmıştır. Bu in vitro çalışmadaki amaç, CDPP ve TQ kombinasyonunun MCF-7 hücre hattı üzerindeki antiproliferatif etkisini araştırmaktır. Bu çalışmada, MCF-7 hücreleri, 24 saat boyunca farklı TQ, CDDP konsantrasyonları ve timokinon:sisplatin kombinasyonları (TQ+CDDP) ile muamele edildi. Hücre canlılığı tayini için MTT testi ve koloni oluşumunu gözlemlemek için kristal viyole boyama yöntemleri uygulandı. MCF-7 hücre popülasyonunun %50’sini inhibe eden (IC50) değer ve kombinasyon index (CI) değerleri CompuSyn yazılım programı kullanılarak hesaplandı. MCF-7 hücreleri üzerinde TQ, CDDP ve TQ+CDDP’nin etkin dozlarının sırasıyla 58 μM, 32 μM ve 31 μM+17 μg/mL olduğu ve bu kombinasyon dozunun Fa=0.5 (IC50) olduğu durumda kombinasyon indeks değerinin 1.11 olduğu gösterildi. Sonuç olarak, TQ ve CDPP’nin tekli dozlarının MCF-7 meme kanseri hücre hattı üzerinde antiproliferatif etkiye sahip olduğu gösterildi. TQ+CDDP kombinasyonunun ise farklı Fa değerlerine denk gelen sinerjistik veya antagonistik etkilerde MCF-7 meme kanseri hücre hattına karşı antiproliferatif etki gösterdiği gözlemlendi.

Anahtar Kelimeler:

Timokinon, sisplatin, MCF-7, antiproliferatif etki

Evaluation of Antiproliferative Effect of Cisplatin and Thymoquinone Combination on MCF-7 Cells

Breast cancer is the second most common cause of death among women and the most frequently diagnosed cancer type in the worldwide. Cisplatin (CDPP), a chemotherapeutic agent, is used in the treatment of breast cancer, but has serious side effects. In addition, the resistance of breast cancer cells such as MCF-7 to cisplatin complicates the treatment. Thymoquinone (TQ), a phytochemical compound found in black cumin, has been applied and investigated as an anticancer agent in different studies. The aim of this in vitro study is to investigate the antiproliferative effect of CDPP and TQ combination on MCF-7 cell line. In this study, MCF-7 cells were treated with different concentrations of TQ, CDDP and thymoquinone:cisplatin combinations (TQ+CDDP) for 24 hours. MTT test were carried out for the cell viability determination and crystal violet staining methods were applied to observe colony formation. The IC50 value and combination index (CI) values that inhibit 50% of the MCF-7 cell population were calculated using the CompuSyn software program. It was shown that the IC50 dose of TQ, CDDP and TQ+CDDP on MCF-7 cells were 58 μM, 32 μM and 31 μM+17 μg/mL, respectively, and the CI value was 1.11. In conclusion, single doses of TQ and CDPP were shown to have antiproliferative effects on the MCF-7 breast cancer cell line. It was observed that the combination of TQ+CDDP showed an antiproliferative effect against MCF-7 breast cancer cell line in synergistic or antagonistic effects corresponding to different Fa values.

Keywords:

Thymoquinone, Cisplatin, MCF-7, antiproliferative effect,

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