Cevahir ALTINKAYNAK, Nalan ÖZDEMİR, Ata ESKİN, Merve TÜRK

Tenebrio molitor Linnaeus, 1758 (Coleoptera: Tenebrionidae) Larvasında Gerçekleştirilen Yeni Bir Toksik Araştırma: Floresan Bakır Fosfat Nano Çiçekler

Son zamanlarda birçok deneysel uygulamada yaygın olarak kullanılan organik-inorganik hibrit nano çiçekler ticarileşme potansiyeline sahip yeni malzemelerdir. Ancak bu nano çiçeklerin toksik etkileri hala bilinmemektedir. Bu çalışmada, ilk defa organik-inorganik hibrit nano çiçek sentez metodu kullanılarak floresan bakır fosfat nano çiçekler (FCPnfs) sentezlendi, morfolojik özellikleri (akridin orange miktarı, şekli, boyutu) analiz edildi. Sonuçlar, farklı miktarda akridin orange’ın farklı morfolojiye neden olduğu ve organik bileşenin nano çiçeklerin içinde homojen bir şekilde dağıldığını gösterdi. Sentezlenen malzemelerin karakterizasyonları SEM, EDX, XRD ve FTIR teknikleri ile gerçekleştirildi. Model organizma olarak seçilen Tenebrio molitor Linnaeus, 1758 (Coleoptera: Tenebrionidae) larvalarında sentezlenen FCPnfs'lerin toksik etkileri, saf bakır fosfat nano çiçekleriyle (CPnfs) karşılaştırmalı olarak incelendi. Larvalara nano çiçekler, 0.001, 0.005, 0.010, 0.025, 0.050, 0.100 mg/10µl olacak şekilde farklı dozlarda zorla besleme yapıldı. Yapılan probit analizine göre, FCPnfs'in LC50 değeri 0.490 mg/10 µl iken LC99 değeri 0.145 mg/10 µl bulundu. Öte yandan, CPnfs'nin LC50 değeri 0.066 mg/10 µl iken LC99 değeri 0.172 mg/10 µl bulundu. Elde edilen toksik veriler değerlendirildiğinde; Tenebrio molitor larvalarının CPnfs'yeFCPnfs'den biraz daha fazla direnç gösterdiği bulundu. Bu bulgular, organik-inorganik hibrit nano çiçek yöntemi kullanılarak sentezlenecek yeni malzemelerin iskelet yapısının toksik etkisinin bilinmesi hakkında araştırmacılara yön gösterecektir.

Fluorescent Copper Phosphate Nanoflowers: A Novel Toxicity Investigation Study Based On Tenebrio molitor Linnaeus, 1758 (Coleoptera: Tenebrionidae) Larvae

Organic-inorganic hybrid nanoflowers with commercialization potential are the novel materials and are widelyused in many experimental applications, recently. However their potential toxicity values of these nanoflowers arestill unknown. In this study, fluorescent copper phosphate nanoflowers (FCPnfs) were first synthesized toconstructed via organic-inorganic hybrid nanoflower synthesis method. The morphological features of FCPnfs(acridine orange amount, shape, size) were analyzed. The results showed that different amount of acridine orangecaused different morphology and the organic component were homogeneously distributed inside the nanoflowers.These materials were characterized by SEM, EDX, XRD, and FTIR techniques. The toxic effects of the asprepared FCPnfs were investigated in Tenebrio molitor Linnaeus, 1758 (Coleoptera: Tenebrionidae) larvaecompared to pure copper phosphate nanoflowers (CPnfs). Nanoflowers were force feed to larvae at the differentdoses of 0.001, 0.005, 0.010, 0.025, 0.050, 0.100 mg/10 μl. According to probit assay, LC50 and LC99 values ofFCPnfs were found 0.490 and 0.145 mg/10 μl, respectively. On the other hand, LC50 and LC99 values of CPnfswere detected 0.066 and 0.172 mg/10 μl, respectively. It was found that the insect exhibited slightly moreresistance to CPnfs than FCPnfs when compared to both chemical toxic values. These findings will be offer a newinsight into the researchers about knowing the toxic effect of the skeletal structure of new materials to besynthesized using the organic-inorganic hybrid nanoflower method.

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[1] Altinkaynak C., Tavlasoglu S., Özdemir N., Ocsoy I. 2016. A New Generation Approach In Enzyme Immobilization: Organic-İnorganic Hybrid Nanoflowers With Enhanced Catalytic Activity And Stability. Enzyme Microb. Technol., 93-94:105-12.
[2] Wang R., Zhang Y., Lu D., Ge J., Liu Z., Zare R.N. 2013. Functional Protein-Organic/Inorganic Hybrid Nanomaterials. Wiley Interdiscip Rev. Nanomed Nanobiotechnol, 5 (4): 320-328.
[3] Lee S.W., Cheon S.A., Kim M.I., Park T.J. 2015. Organic-Inorganic Hybrid Nanoflowers:Types, Characteristics, And Future Prospects. J. Nanobiotechnology, 13: 54.
[4] He X., Chen L., He Q., Xiao H., Zhou X., Ji H. 2017. Self-Assembled Metalloporphyrins– Inorganic Hybrid Flowers And Their Application To Efficient Epoxidation of Olefins. Journal of Chemical Technology & Biotechnology, 92 (10): 2594-2605.
[5] Gulmez C., Altinkaynak C., Özdemir N., Atakisi O. 2018. Proteinase K hybrid nanoflowers (PhNFs) as a novel nanobiocatalytic detergent additive. International Journal of Biological Macromolecules 119: 803-810.
[6] Ge J., Lei J., Zare R.N. 2012. Protein-Inorganic Hybrid Nanoflowers. Nat. Nanotechnol, 7 (7): 428-432.
[7] Yu Y., Fei X., Tian J., Xu L., Wang X., Wang Y. 2015. Self-Assembled Enzyme-Inorganic Hybrid Nanoflowers and Their Application to Enzyme Purification. Colloids Surf B Biointerfaces, 130: 299-304.
[8] Jing M., Fei X., Ren W., Tian J., Zhi H., Xu L., Wang X., Wang Y. 2017. Self-Assembled Hybrid Nanomaterials With Alkaline Protease And A Variety of Metal Ions, RSC Advances, 7 (76): 48360-48367.
[9] Nadar S.S., Gawas S.D., Rathod V.K. 2016. Self-Assembled Organic-Inorganic Hybrid Glucoamylase Nanoflowers With Enhanced Activity and Stability. International Journal of Biological Macromolecules, 92: 660-669.
[10] Altinkaynak C., Tavlasoglu S., Kalin R., Sadeghian N., Ozdemir H., Ocsoy I., Özdemir N. 2017. A Hierarchical Assembly of Flower-Like Hybrid Turkish Black Radish Peroxidase-Cu2+ Nanobiocatalyst And Its Effective Use in Dye Decolorization. Chemosphere, 182: 122-128.
[11] Ildiz N., Baldemir A., Altinkaynak C., Özdemir N., Yilmaz V., Ocsoy I. 2017. Self Assembled Snowball-Like Hybrid Nanostructures Comprising Viburnum opulus L. Extract and Metal İons For Antimicrobial and Catalytic Applications. Enzyme and Microbial Technology, 102: 60-66.
[12] Altinkaynak C., Kocazorbaz E., Özdemir N., Zihnioglu F. 2018. Egg White Hybrid Nanoflower (EW-Hnf) With Biomimetic Polyphenol Oxidase Reactivity: Synthesis, Characterization And Potential Use in Decolorization of Synthetic Dyes. Int. J. Biol. Macromol, 109: 205-211.
[13] Kim K.H., Jeong J-M., Lee S.J., Choi B.G., Lee K.G. 2016. Protein-Directed Assembly Of Cobalt Phosphate Hybrid Nanoflowers. J. Colloid Interface Sci., 484: 44-50.
[14] Zhang Z., Zhang Y., He L., Yang Y., Liu S., Wang M., Fang S., Fu G. 2015. A Feasible Synthesis of Mn3(PO4)2@BSA Nanoflowers and Its Application as the Support Nanomaterial for Pt Catalyst. Journal of Power Sources, 284: 170-177.
[15] Zhang B., Li P., Zhang H., Wang H., Li X., Tian L., Ali N., Ali Z., Zhang Q. 2016. Preparation of Lipase/Zn3(PO4)2 Hybrid Nanoflower and its Catalytic Performance as an Immobilized Enzyme. Chemical Engineering Journal, 291: 287-297.
[16] Wang X., Shi J., Li Z., Zhang S., Wu H., Jiang Z., Yang C., Tian C. 2014. Facile One-Pot Preparation of Chitosan/Calcium Pyrophosphate Hybrid Microflowers. ACS Applied Materials & Interfaces (ACS Publications), 6(16):14522-14532.
[17] Jiao J., Xin X., Wang X., Xie Z., Xia C., Pan W. 2017. Self-Assembly Of Biosurfactant–Inorganic Hybrid Nanoflowers As Efficient Catalysts For Degradation Of Cationic Dyes, RSC Advances, 7 (69):43474-43482.
[18] Li J., Schiavo S., Xiangli D., Rametta G., Miglietta M.L., Oliviero M., Changwen W., Manzo S. 2018. Early Ecotoxic Effects of ZnO Nanoparticle Chronic Exposure in Mytilus Galloprovincialis Revealed by Transcription of Apoptosisand Antioxidant-Related Genes. Ecotoxicology, 27 (3): 369-384.
[19] Gebel T. 2012. Small Difference in Carcinogenic Potency Between GBP Nanomaterials and GBP Micromaterials. Arch. Toxicol, 86 (7): 995-1007.
[20] Sun X., Chen B., Bin Xia N., Han Q., Zhu L., Qu K. 2017. Are CuO Nanoparticles Effects on Hemocytes of the Marine Scallop (Chlamys Farreri) Caused by Particles And/Or Corresponding Released Ions?. Ecotoxicol Environ. Saf., 139: 65-72.
[21] Adam N., Vakurov A., Knapen D., Blust R. 2015. The Chronic Toxicity of CuO Nanoparticles and Copper Salt to Daphnia Magna. J. Hazard Mater., 283: 416-422.
[22] Hoseini S.M., Hedayati A., Taheri Mirghaed A., Ghelichpour M. 2016. Toxic Effects of Copper Sulfate and Copper Nanoparticles on Minerals, Enzymes, Thyroid Hormones and Protein Fractions of Plasma and Histopathology in Common Carp Cyprinus carpio. Exp. Toxicol Pathol., 68 (9): 493-503.
[23] Rajput V., Minkina T., Fedorenko A., Sushkova S., Mandzhieva S., Lysenko V., Duplii N., Fedorenko G., Dvadnenko K., Ghazaryan K. 2018. Toxicity of Copper Oxide Nanoparticles on Spring Barley (Hordeum Sativum Distichum). Science of the Total Environment, 645: 1103- 1113.
[24] Braz-Mota S., Campos D.F., MacCormack T.J., Duarte R.M., Val A.L., Almeida-Val V.M.F. 2018. Mechanisms of Toxic Action of Copper and Copper Nanoparticles in Two Amazon Fish Species: Dwarf Cichlid (Apistogramma agassizii) and Cardinal Tetra (Paracheirodon axelrodi). Sci. Total Environ., 630: 1168-1180.
[25] Collins L. 2012. Toxicity of Moist Snuff and Impact on Various Stages of Darkling Beetles. Biology and Biotechnology, 7.
[26] Ramarao N., Nielsen-Leroux C., Lereclus D. 2012. The Insect Galleria Mellonella As A Powerful İnfection Model to Investigate Bacterial Pathogenesis. J. Vis. Exp., 70: 4392.
[27] Dere B., Altuntaş H., Nurullahoğlu Z.U. 2015. Insecticidal and Oxidative Effects of Azadırachtın on the Model Organism Galleria mellonella L. (Lepidoptera: Pyralidae). Arch. Insect. Biochem Physiol, 89 (3): 138-152.
[28] Manente S., De Pieri S., Iero A., Rigo C., Bragadin M. 2008 A Comparison Between The Responses Of Neutral Red And Acridine Orange: Acridine Orange Should Be Preferential And Alternative To Neutral Red As A Dye For The Monitoring Of Contaminants By Means Of Biological Sensors. Anal. Biochem., 383 (2): 316-319.
[29] Altuntaş H., Duman E., Şanal Demirci S.N., Ergin E. 2016. Toxicological And Physiological Effects Of Ethephon On The Model Organism, Galleria mellonella L. 1758 (Lepidoptera: Pyralidae). Turkish Journal of Entomology, 40 (4).
[30] Nishi M., Hiruma H., Sasamoto H., Iwamura M., Isonaka R., Baba S. 2012. Cytocidal Effects Of Acridine Orange Evoked By Blue Light On Human Bladder Cancer Cells. Kitasato Med. J., 42: 128-137.
[31] Ali T.H., Abed A.A., Ellah A.A. 2016. Determination Of The Lethal Concentration 50% (Lc50) Of Cadmium Chloride in Mosquito Fish Gambusia Holbrooki. Tikrit Journal of Pure Science, 21: 1.
[32] Calabrese E.J., Baldwin L.A. 2001. U-Shaped Dose-Responses in Biology, Toxicology And Public Health. Annu. Rev. Public Health, 22: 15-33.
[33] Carmona E.R., Inostroza-Blancheteau C., Obando V., Rubio L., Marcos R. 2015. Genotoxicity Of Copper Oxide Nanoparticles in Drosophila melanogaster. Mutat. Res. Genet. Toxicol Environ. Mutagen, 791: 1-11.
[34] Baeg E., Sooklert K., Sereemaspun A. 2018. Copper Oxide Nanoparticles Cause a DoseDependent Toxicity via Inducing Reactive Oxygen Species in Drosophila. Nanomaterials (Basel), 8: 10.
[35] Sezer Tunçsoy B., Ozalp P. 2016. Toxic Effects Of Copper Oxide Nanoparticles In Midgut And Fat Body Of Galleria mellonella (Lepidoptera: Pyralidae). Toxicology Letters, 258: 270.