Sapanca Gölü (Sakarya) Havzası Toprak ve Sedimentlerinden Streptomyces Cinsi Bakterilerin İzolasyonu, Karakterizasyonu ve Teşhisi

Bu çalışmada, Sakarya ili sınırlarında bulunan Sapanca Gölü havzası toprak ve sedimentlerinden Streptomycescinsi bakterileri izole edilerek karakterizasyon ve teşhisi yapılmıştır. Toplam 6 farklı lokaliteden alınan 12 topraknumunesinin fiziko-kimyasal analizleri yapılmış ve pH’larının 6,12 ile 7,58 değerleri arasında olduğu saptanmıştır.Saflaştırılan 60 Streptomyces izolatı havasal, misel ve difüziye pigment rengi esas alınarak 4 ana gruba ayrılmıştır.Streptomyces izolatlarına, antibiyotik duyarlılığı, degredasyon aktivitesi, büyüme, azot ve karbon kaynağıkullanımı testleri uygulanmıştır. Tüm izolatların fenotipik ve biyokimyasal karakterleri belirlenerek IDENTAXbilgisayar programına bu veriler aktarılmış, cins içerisindeki pozisyonları belirlenerek teşhisleri yapılmıştır. Teşhisedilenler türler arasında Streptomyces cyaneus, Streptomyces filipinensis, Streptomyces exfoliatus, Streptomyceschromogenus, Streptomyces chromofuscus, Streptomyces griseoruber, Streptomyces antibioticus, Streptomyceslydicus, Streptomyces lavendulae ve Streptomyces purpureus türleri bulunmaktadır. Teşhisleri tamamlananStreptomyces izolatlarının test sonuçları MVSP 3.2 bilgisayar programı ile birbirleri arasındaki benzerlikdendogramları oluşturulmuştur. Renk kümelerini temsilen seçilen 4 suşun DNA izolasyon ve dizi analizleriyapılmış ve 27F ile 1492R evrensel primerleri kullanılarak 16S rDNA geni kısmi olarak PCR ile çoğaltılmıştır.Bu dört bakterinin GenBank veri tabanındaki türlerle olan akrabalık dereceleri ve genetik pozisyonlarıdendogramlar şeklinde ortaya konmuştur.

Isolation, Molecular Characterization and Identification of Bacteria the Genus Streptomyces from Basin Sedimentary Soil of Sapanca Lake (Sakarya)

In this study, Streptomyces bacteria were characterized and identified after being isolated from the soil and sediments of Lake Sapanca in Sakarya province. Physico-chemical analyzes of 12 soil samples taken from 6 different localities on total, were carried out. It was determined that the pH values of the soil samples were between 6.12 and 7.58. The purified 60 Streptomyces isolates were divided into 4 main groups based on air, micelle and diffuse pigment colour. Streptomyces isolates were analysed and tested for antibiotic susceptibility, degradation activity, growth, nitrogen and carbon source use. Phenotypic and biochemical characteristics of all isolates were determined and these data were transferred to IDENTAX computer program and their positions were identified and diagnosed. Species identified include Streptomyces cyaneus, Streptomyces filipinensis, Streptomyces exfoliatus, Streptomyces chromogenus, Streptomyces chromofuscus, Streptomyces griseoruber, Streptomyces antibioticus, Streptomyces lydicus, Streptomyces lavendulae ve Streptomyces purpureus. The test results of the Streptomyces isolates, which have been diagnosed, were compared with the MVSP 3.2 computer program to establish similarity dendograms. DNA isolation and sequence analysis of 4 selected colonies were performed and 27F and 1492R universal primers were used to amplify the 16S rDNA gene partially by PCR. The genesis and genetic positions of these four bacteria with the species in the GenBank database are presented as dendograms.

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