Karbontetraklorür İle Karaciğer Hasarı Oluşturulan Ratlarda Tribulus terrestris’in Antihiperlipidemik Etkisinin Araştırılması

Bu araştırmada, karbon tetraklorür ile deneysel olarak oluşturulan hepatotoksisite ile ratların kan lipidparametrelerine karşı güçlü antioksidan etkisine sahip olan Tribulus terrestris’in koruyucu etkinliğinin veantihiperlipidemik rolünün araştırılması amaçlanmıştır. Çalışmada 35 adet 8-12 haftalık Wistar-Albino erkek ratkullanılmıştır. Çalışma beş grupa ayrılıp her grupta 7 adet ratla başlanmıştır. Kontrol grubundaki ratlar 14 günstandart yemle beslenmiştir. Diğer gruplardaki ratlara standart yem ve 14 gün boyunca 0,8 ml/kg CCI4intraperitoneal olarak uygulanmıştır. 3, 4 ve 5 gruplarda ki ratlara ise intraperitoneal olarak uygulanan 0,8 ml/kgCCI4’e sonra 8 hafta boyunca Tribulus terrestris bitki ekstraktı verilmiştir. Deney sonunda tüm sıçanlar uygunortam ve şartlarda dekapite edilerek ve çalışmanın sonuçlarının inceleneceği parametreler için uygun kan örneklerialınmıştır. Alınan kan örnekleri jelli düz biyokimya tüplerine alınacak, 4000 rpm’de 5-10 dakika santrifüj edilerekserum elde edilmiştir. Elde edilen serumlar otoanalizör yardımıyla lipid parametreleribelirlenmiştir. Karbontetraklorür ile karaciğer hasarı oluşturan grup ile kontrol grubu lipid parametreleri totalkolesterol, trigliserit, düşük dansiteli lipoprotein kolesterol (LDL-K), çok düşük dansiteli lipoprotein kolesterol(VLDL-K) ve yüksek dansiteli lipoprotein kolesterol (HDL-K) değerlerinin arasında istatiksel bir fark olduğusaptandı (P0.05). CCI4’ün neden olduğu oksidatif stres ve lipit peroksidasyonusonucu oluşturulan hiperlipidemiye karşı Tribulus Terrestris’in bitkisinin önemli düzeyde azaltıcı etkisinin olduğubelirlenmiştir.

Examination of the Antihyperlipidemic Effect of Tribulus terrestris in LiverDamaged Rats with Carbontetrachloride

The aim of this study was to investigate the protective effect and antihyperlipidemic role of Tribulus Terrestris, a potent antioxidant effect of hepatotoxicity in experimental rats on carbon tetrachloride and lipid parameters. 35 Wistar-Albino male rats aged 8-12 weeks were used in the study. Rats were divided into 5 groups of 7 animals per group and rats in the control group were fed standard food for 14 days. In groups 3, 4 and 5 rats, Tribulus terrestris plant extracts were given 8 weeks after intraperitoneal administration of 0.8 ml / kg CCI4. All rats were cut in the appropriate medium and blood samples were taken for the parameters to be monitored. Blood samples from were taken with glandular biochemical tubes and centrifuged at 4000 rpm for 5-10 minutes to obtain serum. Lipid parameters were determined using an autoanalyzer. The lipid parameters of carbon tetrachloride and liver damage group and control group were found between total cholesterol, triglyceride, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol and high-density lipoprotein cholesterol significantly (P 0.05). Oxidative stress and lipid peroxidation caused by CCI4 have been identified as an important inhibitory effect of Tribulus terrestris in against liver damage and hyperlipidemia.

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