Songül ÇANAKCI GÜLENGÜL, Didem DEVECİ, Fadime KARABULUT

Mısır (Zea mays L.) Fidelerinde Kadmiyum Toksisitesi ile Nitrik Oksit Arasındaki Biyokimyasal İlişkiler

Bu çalışmada, 15 günlük mısır (Zea mays L.) fidelerine, önceden farklı sodyum nitroprussid (SNP) (25 ve 50 µM)konsantrasyonları uygulandıktan sonra, bu fidelerin farklı kadmiyum (25,50 ve 75 µM) konsantrasyonlarına karşıverdiği biyokimyasal cevaplar araştırıldı. Fidelere yapılan tüm uygulamalar hidroponik ortamda uygulandı.Kadmiyum (Cd) uygulanan mısır fidelerinin köklerinde ve yapraklarında SNP ön uygulamasız fidelerde, kontrolekıyasla okside glutatyon (GSSG) ve redükte glutatyon (GSH) miktarlarında artma ve SNP ön uygulamalı fidelerdeise azalma tespit edildi. Fidelere uygulanan kadmiyum konsantrasyonları arttıkça, hem SNP ön uygulamasız, hemde SNP ön uygulamalı fidelerde kontrol grubu fidelerine kıyasla 16:0 (palmitik asit) yapraklarda ve köklerdeartmıştır. Tek başına SNP uygulamalarında, 16:0 üzerinde kontrole kıyasla, 50 µM SNP daha az bulunmuştur. Cduygulamaları ile SNP ön uygulamasız ve SNP ön uygulamalı fidelerde 16:1 (palmioleik asit) yapraklarda genelolarak artarken, bazı konsantrasyonlarda SNP ön uygulamasıyla bu artış hafifletilmiştir. Cd uygulamaları ile SNPön uygulamasız ve SNP ön uygulamalı fidelerde 18:0 (stearik asit) köklerde ve 18:2 (linoleik asit) kök veyapraklarda artmıştır. Cd uygulamaları ile SNP ön uygulamasız fidelerde 18:3 (linolenik asit) yaprakta azalırken;SNP ön uygulamalı bazı fidelerde ise 18:3 miktarı yapraklarda artmıştır. Genel olarak 50 µM SNP önuygulamasının Cd toksisitesini baskılama da 25 µM SNP’ den daha başarılı bulundu.

Investigation of The Relationship Between Cadmium’s Toxicity and Nitric Oxide in Corn (Zea mays L.) Plants

In this study, after 15 day old corn (Zea mays L.) seedlings apply firstly to different concentrations (25 and 50 μM) of sodium nitoprusside (SNP), biochemical responses of these seedlings to different concentrations (25, 50 and 75 μM) of cadmium are examined. All applications made to seedlings were in hydroponic surroundings. In the amount of reduced glutathione (GSH) and oxide glutathione (GSSG) were increased in the roots and shoots of cadmium (Cd)-administered corn seedlings in SNP non-pretreatment seedlings once compared to the control and they was decreased in SNP pretreatment seedlings. As the cadmium concentrations applied to the seedlings increased both the SNP non-pretreatment and the SNP pretreatment seedlings, 16:0 (palmitic acid) increased in the roots and shoots once compared to the control group seedlings. Only In SNP applications was found less than 50 μM SNP at 16:0 once compared to control. With Cd applications, SNP non-pretreatment and SNP pretreatment seedlings generally increased at 16:1 (palmioleic acid) shoots, while at some concentrations this increase was alleviated by pretreatment of SNP. With Cd applications, the 18:0 (stearic acid) increased in roots and the 18:2 (linoleic acid) increased in shoots and roots; at the SNP non-pretreatment and the SNP pretreatment seedlings. With Cd applications, 18:3 (linolenic acid) decreased in shoots in the SNP non-pretreatment shoots; in some the SNP pretreatment seedlings, 18:3 increased by an amount of shoots. In general, 50 μM SNP pretreatment was found more successful than 25 μM SNP in suppressing Cd toxicity.

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