Determination of MIC and MBC Values Using Different Extraction Methods in Plants of Nigella Sativa, Cuminum Cyminum and Pimpinella Anisum L. Samples from Kırıkkale Region

In our study, it was aimed to investigate the antimicrobial effect of 6 plant extracts obtained with methanol by using maceration and soxlet methods from 3 plant species Nigellla sativa (Black seed), Cuminum cuminum (cumin) and Pimpinella anisum (anise) grown in Kırıkkale province by liquid microdilution method against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). All of the extracts of Nigellla sativa (black cumin), Cuminum cuminum (Cuminum) and Pimpinella anisum (Anise) prepared by the maceration and soxalet method showed antibacterial effects against both bacteria (inhibitory and bactericidal). it was determined that the antibacterial effect of the extracts obtained by the maceration method was higher than the extracts obtained by the soxalet method.

Keywords:

MIC, MBC, METHANOL,

PDF

___

[1] C. Cordeiro Marina, “Phytochemical and pharmacological investigations of Bridelia retusa Spreng,” 2012.
[2] A. G. Fankam, J. R. Kuiate, and V. Kuete, “Antibacterial activities of Beilschmiedia obscura and six other Cameroonian medicinal plants against multi-drug resistant Gram-negative phenotypes,” BMC Complement. Altern. Med., vol. 14, no. 1, p. 241, 2014.
[3] M. Lahlou, “The success of natural products in drug discovery,” Pharmacol. Pharm., vol. 04, no. 03, pp. 17–31, 2013.
[4] M. Saxena , J. Saxena , R. Nema , D. Singh , A. GuptaJ.Eczacılık. fitokimya, 1 ( 2013 )
[5] E. M. Abdallah, “Black seed (Nigella sativa) as antimicrobial drug: A mini-review,” Novel Approaches in Drug Designing & Development, vol. 3, no. 2, pp. 9–13, 2017.
[6] R. P. Singh, G. H.v., and M. K, “Cuminum cyminum – A popular spice: An updated review,” Pharmacogn. J., vol. 9, no. 3, pp. 292–301, 2017.
[7] A. Amer and U. Aly, “Antioxidant and antibacterial properties of anise (Pimpinella anisum L.),” Egypt. Pharm. J., vol. 18, no. 1, p. 68, 2019.
[8] M. Azizi, H. K. Farshchi, F. Oroojalian, and H. Orafaee, “Green synthesis of silver nano-particles using Kelussia odoratissima mozaff. Extract and evaluation of its antibacterial activity,” Journal of Agricultural Science and Technology, vol. 19, no. 3, pp. 681–691, 2017.
[9] F. Erci, R. Cakir-Koc, and I. Isildak, “Green synthesis of silver nanoparticles using Thymbra spicata L. var. spicata (zahter) aqueous leaf extract and evaluation of their morphology-dependent antibacterial and cytotoxic activity,” Artif. Cells Nanomed. Biotechnol., vol. 46, no. sup1, pp. 150–158, 2018.
[10] S. Singh, S. S. Das, G. Singh, C. Schuff, M. P. de Lampasona, and C. A. N. Catalán, “Composition, in vitro antioxidant and antimicrobial activities of essential oil and oleoresins obtained from black cumin seeds (Nigella sativa L.),” Biomed Res. Int., vol. 2014, p. 918209, 2014.
[11] Q. Chen et al., “In vitro comparison of antioxidant capacity of cumin (Cuminum cyminum L.) oils and their main components,” Lebenson. Wiss. Technol., vol. 55, no. 2, pp. 632–637, 2014.
[12] A. Shojaii and M. Abdollahi Fard, “Review of Pharmacological Properties and Chemical Constituents of Pimpinella anisum,” ISRN Pharm., vol. 2012, p. 510795, 2012.
[13] K. O. Saygi, B. Kacmaz, and S. Gul, “Antimicrobial activities of coriander seed essential oil and silver nanoparticles,” Dig. J. Nanomater. Biostructures, vol. 16, no. 4, pp. 1527-1535, 2021.