Bir Aylık ve Beş Aylık Erkek Ratlarda Medulla Spinalis’in Servikal Segmenti Üzerine Yapılan Morfolojik ve Stereolojik Bir Çalışma

Bu çalışmada ratlarda medulla spinalis’in servikal bölümünün ak ve gri madde ve tüm dokunun hacim yoğunlukları stereolojik yöntem kullanılarak araştırıldı. Materyal olarak 1 aylık ve 5 aylık olmak üzere iki farklı yaş grubundan altışar adet erkek Wistar Albino ırkı ratlar kullanıldı. Materyaller % 10’luk tamponlu formaldehitle perfüze edilerek tespit edildi. Daha sonra ratlar diseke edildi ve medulla spinalis’leri açığa çıkarıldı. Çalışmada 1 aylık ve 5 aylık ratların medulla spinalis’lerinin cervical bölümlerinden segmentler elde edildi. Kesitler alınırken ilk 15 kesit arasından rasgele bir tanesi seçildi. Onu takip eden her 50.kesit sistematik rasgele örnekleme yöntemiyle belirlendi. Böylelikle bir hayvanın medulla spinalis’inin cervical segment’inden 5µm kalınlığında 8-10 kesit alındı. Bu kesitler hematoksilen eosin boyama tekniği ile boyanarak mikroskop altında fotoğraflandı. Cavalieri Prensibi kullanılarak noktalı alan cetveli sayesinde medulla spinalis’in cervical bölümünün her bir segmentinde tüm dokunun, ak madde ve gri madde kısımlarının hacim yoğunlukları hesaplandı. Noktalı alan sayımı için SHTEREOM 1.5 paket programı kullanıldı. Ayrıca araştırmada cervical segmentin tüm hacminin, ak madde ve gri madde hacim değerleri ve bu hacim değerlerinin birbirlerine oranları hesaplanarak değerlendirildi.

A Morphological and Stereological Study on Cervical Spinal Cord of One and Five Months Age Male Rat

In this study volume density of gray and white matter of cervical segments of spinal cords of rats were investigatedusing stereological method. Twelve male Wistar albino rats were used in the study as two different age groups 1month and 5 months. All animals were fixed by perfusing 10% buffered formalin. Rats were dissected and spinalcords of rats were removed. In the study cervical segments of 1 and 5 months age rats were obtained. One of thefirst 15 section were selected randomly when the sections were taken. And following every 50th section wasdetermined by systematic random sampling. Thus, 8-10 sections of 5µm thickness were obtained from cervicalsegments of each animal’s medulla spinalis. These sections were stained by hemotoxylin eosin and they werephotographed at microscope. Densities of volumes of all tissue of cervical segments of whole spinal cord and whiteand gray matters were calculated using dotted area ruler by Cavalieri Principle. SHTEREOM 1.5 packageprogramme was used for counting dotted area. In addition, the volume vales of total cervical segment, volumevalues of the white matter and the gray matter and the ratios of these volume values to each other were evaluatedin the study.

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