Arabidopsis thaliana Bitkisi ile Pseudomonas putida Bakterisi Arasındaki Etkileşimin in vitro Koşullarda Belirlenmesi

A. thaliana genomu dizilenmiş genetik, biyokimyasal ve biyoteknolojik çalışmalarda yaygın olarak kullanılan model bir bitkidir. P. putida bakterisi ise bitki köklerinde kolonize olabilen, genomu dizilenmiş, bitki büyümesini teşvik eden, patojenik olmayan ve bitki-bakteri etkileşimlerinin anlaşılması için model olmaya aday rizosferik bir bakteridir. Bu nedenle, bu çalışmanın amacı A. thaliana’nın in vitro çimlenmesi ve fideciklerinin büyümesi üzerine P. putida’nın etkisinin değerlendirilmesidir. Bu amaçla, yüzey sterilasyonu yapılmış A. thaliana tohumlarının inokülasyonu için 2x103-2x105 CFU/ml aralığındaki bakteri konsantrasyonları pipetleme metodu kullanılarak denenmiştir. Ayrıca, aynı bakteriyel süspansiyonlar 3, 5, 10 ve 14 günlük in vitro koşullarda çimlendirilmiş fideciklere de uygulanmıştır. Bu araştırma alanı, bitki-bakteri etkileşimlerinde inokülasyonun öneminin anlaşılmasına önemli bir yarar ve bunun yanı sıra daha sonraki kapsamlı çalışmalar için bir katkı sağlayacaktır. Dahası, sonrasında bitki-bakteri etkileşimleri aracılığıyla biyoremediasyonun başarılmasına yardımcı olacaktır.

Anahtar Kelimeler:

Arabidopsis, Pseudomonas putida

Approximate Solutions of Singularly Perturbed Nonlinear Ill-posed and Sixth-order Boussinesq Equations with Hybrid Method

The aim of this paper is to obtain the approximate solution of singularly perturbed ill-posed and sixth-order Boussinesq equation by hybrid method (differential transform and finite difference method) as a different alternative method. Differential transform method is applied for ? −time variable and the finite difference method (central difference approach) is applied for ? −position variable. Two examples are presented to demonstrate the efficiency and reliability of the hybrid method. Numerical results are given and compared with exact solution and in literature RDTM solution. The numerical data show that hybrid method is a powerful, quite efficient and is practically well suited for solving nonlinear singular perturbed Boussinesq equations.

Keywords:

Sixth-order Boussinesq Equation, Differential Transform Method Finite Difference Method, Approximate Solution,

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