Türkiye’de Mardin İli Zeytin Alanlarında Bazı Virüslerin Serolojik ve Moleküler Tekniklerle Araştırılması

Güneydoğu Anadolu bölgesinde yer alan Mardin il ve ilçelerindeki zeytin ağaçlarındaki bazı virüs hastalıklarının durumunu araştırmak amacıyla 2010 yılında surveyler yapılmıştır. Virüs simptomu gösteren ağaçlardan ve virüslerin latent olarak taşındıkları göz önüne alınarak simptomsuz ağaçlardan da tesadüfi olarak toplam 170 sürgün örneği toplanmıştır. Alınan örnekler Olive latent-1 virus (OLV-1, Zeytin latent-1 virüs), Olive latent-2 virus (OLV-2, Zeytin latent-2 virüsü), Cherry leaf roll virus (CLRV, Kiraz yaprak kıvrılma virüsü), Arabis mosaic virus (ArMV, Arabis mozaik virüs), Strawberry latent ringspot virus (SLRSV, Çilek latent halkalı leke virüsü), Olive latent ringspot virus (OLRSV, Zeytin latent halkalı leke virüsü) ve Cucumber mosaic virus (CMV, Hıyar mozaik virüsü), Olive latent-3 virus (OLV-3, Zeytin latent-3 virüs)’üne karşı testlere tabi tutulmuştur. Toplanan zeytin örneklerine mevcut poliklonal antiserumlar kullanılarak (DAS-ELISA) serolojik testler (CLRV, ArMV, SLRSV veCMV) ve moleküler metodlar (dsRNA, RT-PCR) uygulanarak virüsler test edilmiştir. DAS-ELISA ve PCR analizlerinde tüm örnekler negatif olarak tespit edilirken, dsRNA anlizlerinde 41 örnekte, dsRNA profilleri tespit edilmiştir. Test edilen zeytin örnekleri araştırılan virüsler açısından temiz bulunurken, bu örneklerin zeytin ağaçlarını enfekte ettiği bilinen diğer virüsler açısından da test edilmesi gerektiği sonucuna ulaşılmıştır.

Research on the Presence of Some Viruses in Olive Orchards Using Serological and Molecular Techniques in Mardin Province of Turkey

Surveys were performed in 2010 in order to investigate the presence of several virus diseases in olive trees grown in Mardin province and counties located in the Southeastern Anatolia. Totally 170 shoot samples were collected from trees showing virus symptoms and also collected randomly symptomless trees considering the possibility of virus presence latently. Olive plant samples were tested against Olive latent-1 virus (OLV-1), Olive latent-2 virus (OLV-2), Cherry leaf roll virus (CLRV), Arabis mosaic virus (ArMV), Strawberry latent ringspot virus (SLRSV), Olive latent ringspot virus (OLRSV), Cucumber mosaic virus (CMV) and Olive latent-3 virus (OLV-3). To identify viral agents, the serological (DAS-ELISA) tests using available polyclonal antisera (SLRSV, ArMV, CLRV and CMV) and molecular methods (dsRNA, RT-PCR) were applied to collected olive samples. According to results of DAS-ELISA and PCR analysis, all samples were found negative whereas dsRNA profiles were observed in 41 samples. All tested samples were found free of tested viruses but it was concluded that they have to be tested against other known viruses of olives.

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