Malatya bölgesi bağlarında Asma yaprak kıvrılma virüsleri (GLRaVs)’nin sürveyi
Asma (Vitis
vinifera L.) ülkemizde yetiştiriciliği yapılan en önemli meyvelerden birisi
olup üretimini sınırlayan faktörlerin başında virüs hastalıklar gelmektedir.
Virüs hastalıkları üzüm üretimini kalite ve kantite bakımından azaltmanın yanı
sıra diğer hastalık etmenleri ile beraber bulunmaları durumunda asmanın
kurumasına kadar ulaşabilen ciddi sonuçlar doğurabilmektedir. Bir virüs kompleksinin
neden olduğu Asma yaprak kıvrılma hastalığı ülkemiz asma
yetiştiriciliği üzerinde önemli bir tehdittir. Bu çalışma, Malatya ilinde
yetiştirilen asmaları etkileyen Asma yaprak kıvırcıklık virüsleri (Grapevine leafroll associated virus, GLRaV)’ini tespit etmek
amacı ile yürütülmüştür. Bu amaçla Malatya ilindeki asma bahçeleri gezilerek etmenin
1, 2, 3, 4 ve 6 nolu ırkları araştırılmıştır. 2016-2017 yıllarında Arapgir,
Darende ve Battalgazi ilçelerini kapsayan survey çalışmalarında güdümlü
örneklemeler yapılarak asma numuneleri toplanmıştır. Toplanan 241 örneğin
tamamı DAS-ELSA metodu ile testlenmiştir. Testlerde negatif örneğin iki katı ve
yukarısında absorbans (405 nm) değeri veren numuneler infekteli kabul
edilmiştir. Yürütülen test sonuçlarına göre toplam 131 numunenin GLRaV 1 ile,
48 numunenin GLRaV 3 ile ve 4 örneğin ise GLRaV 4 ile infekteli olduğu tespit
edilmiştir. Örneklerin hiçbirinde GLRaV 2 ve GLRaV 6 infeksiyonuna
rastlanmamıştır. Testlenen 45 numunenin GLRaV 1 ve 3 ile, üç numunenin ise GLRaV
1 ve 4 ile karışık infeksiyon halinde bulunduğu tespit edilmiştir.
Survey for Grapevine leafroll viruses (GLRaVs) in Malatya region
Grapevine (Vitis vinifera L.) is one of the most important fruit crops in Turkey
and viral diseases are one of the main problems of grapevine production. They
decrease yield quantity and quality of crops as well as cause death of the plants
in an interaction with other agents. Leafroll disease of grapevine, caused by a
complex of viruses, is considered a serious threat to production across many
grapevine growing regions in Turkey. This study was conducted to determine
the current status of Grapevine leafroll-associated viruses (GLRaVs) in Malatya
province. Grapevine plantations in Malatya province were surveyed for the
presence of Grapevine leafroll-associated viruses (GLRaVs) -1, -2, -3, -4 and -6.
Purposive sampling was done in vineyards of Arapgir, Darende and Battalgazi
districts, which are the primary grapevine producing areas of Malatya during
2016-2017 growing seasons. A total of 241 samples were collected and tested by
DAS-ELISA. Samples giving two fold of absorbance values (405 nm) of negative
control were evaluated as positive. According to the results, 131 samples were
found to be infected with GLRaV-1, whereas 48 samples with GLRaV-3 and -4
samples with GLRaV-4. None of the samples were reacted as positive against
GLRaV-2 and GLRaV-6. Forty five samples were found to be infected with mix
infection of GLRaV-1 and -3, and three samples were found to be infected with
GLRaV-1 and -4 strain 6.
___
- Anonymous 2018. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr. (Accession date: 14.03.2018).
- Akbaş B., Kunter B., Ilhan D., 2007. Occurrence and distribution of Grapevine leafroll-associated viruses 1, 2, 3 and 7 in Turkey. Journal of Phytopathology, 155, 122–124.
- Akbaş B., Kunter B., İlhan D., 2009. Influence of leafroll on local grapevine cultivars in agroecological conditions of Central Anatolia Region. Horticultural Science, 36 (3), 97-104.
- Almeida R., Daane K., Bell V., Blaisdell G., Cooper M., Herrbach E., 2013. Ecology and management of grapevine leafroll disease. Frontiers in Microbiology, 4, 94.
- Atallah S., Gomez M., Fuchs M., Martinson T., 2012. Economic impact of grapevine leafroll disease on Vitis vinifera cv. Cabernet franc in Finger Lakes vineyards of New York. American Journal of Enology and Viticulture, 63, 73-79
- Cabaleiro C., Segura A., 2006. Temporal analysis of
grapevine leafroll associated virus 3 (GLRaV-3) epidemics.
European Journal of Plant Pathology, 114, 441-446.
- Cabaleiro C., Segura A., Berrios J.G., 1999. Effects of
grapevine leafroll-associated virus 3 on the physiology
and must of Vitis vinifera L. cv. Albarino following
contamination in the field. American Journal of Enology
and Viticulture, 50 (1), 40-44
- Çağlayan K., 1997. lncidence of Grapevine leafroll,
Grapevine virus A and Tomato black ring virus in the
vineyards of Hatay province. The Journal of Turkish
Phytopathology, 121-129.
- Charles J.G., Cohen D., Walker J.T.S., Forgie S.A., Bell V.A.,
Breen K.C., 2006. A review of Grapevine leafroll-associated
virus type 3 (GLRaV-3) for the New Zealand wine industry.
Report to New Zealand wine growers. HortResearch Client
Report, No. 18447, HortResearch, Palmerston North, New
Zealand, 101 pp.
- Çiftçi O., Ulubaş Serçe Ç., Güler B., 2015. Survey of
grapevine viruses in the East and Southeast Regions of
Turkey. 18th Congress of the International Council for the
Study of Virus and VirusLike Diseases of the Grapevine
(ICVG), September 7-11, 2015, Ankara-Turkey, 200.
- Clark M.F., Adams A.N., 1977. Characteristics of the
microplate method of enzyme-linked immunosorbent
assay for the detection of plant viruses. Journal of Genetic
Virology, 34 (3), 475-83.
- Erilmez S., Kaya A., 2016. Asma virüslerinin tanılanmasında
DAS-ELISA ve RT-PCR yöntemlerinin duyarlılıklarının
karşılaştırılması. Bitki Koruma Bülteni, 56 (1), 1-14.
- Goszczynski D.E., Kasdorf G.G.F., Pietersen G., 1995.
Production and use of antisera specific to Grapevine
leafroll-associated viruses following electrophoretic
separation of their proteins and transfer to nitrocellulose.
African Plant Protection, 1, 1–8.
- Gugerli P., Brugger J.J., Bovey R., 1984. Líenroulement
de la vigne: mise en Èvidence de particles virales et
dÈveloppement díune mÈthode immuno-enzimatique
pour le diagnostic rapide. Revue Suisse de Viticulture,
Arboriculture, Horticulture, 16, 299-304.
- Gugerli P., Rosciglione B., Brugger J.J., Bonnard S., Ramel
M.E., Tremea F., 1990. Etiological studies and diagnosis
of grapevine leafroll disease improved by monoclonal
antibodies. In: A. Shots (ed.) Monoclonal antibodies in
Agriculture. Proceedings Symposium Perspectives for
monoclonal antibodies in agriculture, Wageningen 1990.
Pudoc, Wageningen, 47-54.
- Krake L.R., 1993. Characterization of grapevine leafroll
disease by symptomatology. The Australian and New
Zealand Wine Industry Journal, 8, 40-44.
- Ling K., Zhu H., Jiang Z., Gonsalves D., 2000. Effective
application of DAS-ELISA for detection of Grapevine
leafroll-associated closterovirus-3 using a polyclonal
antiserum developed from recombinant coat protein.
European Journal of Plant Pathology, 106, 301–309.
- Ling K., Zhu H., Petrovic N., Gonsalves D., 2001.
Comparative effectiveness of ELISA and RT-PCR for
detecting Grapevine leafroll-associated closterovirus-3
in field samples. American Journal of Enology and
Viticulture, 52, 21–27.
- Martelli G.P., 1986. Virus and virus like diseases of the
grapevine in the Mediterranean area. FAO Plant Protection
Bulletin, 34, 25–42.
- Martelli G.P., 1987. Virus and virus-like diseases of
grapevine in Turkey. Rome, FAO: 32.
- Martelli G.P., Boudon-Padieu E., 2006. Directory
of infectious diseases of grapevines. Options
Méditerranéennes, Series B 55, 11-201.
- Martelli G.P., Abou Ghanem-Sabanadzovic N., Agranovsky
A.A., Al Rwahnih M., Dolja V.V., Dovas C.I., Fuchs M.,
Gugerli P., Hu J.S., Jelkmann W., Katis N.I., Maliogka V.I.,
Melzer M.J., Menzel W., Minafra A., Rott M.E., Rowhani
A., Sabanadzovic S., Saldarelli P.,. 2012. Taxonomic
revision of the family Closteroviridae with special reference
to the Grapevine leafroll associated members of the genus
Ampelovirus and the putative species unassigned to the
family. Journal of Plant Pathology, 94 (1), 7-19.
- Martin R.R., Eastwell K.C., Lamprecht S., Tzanetakis
I.E., 2005. Survey for viruses of grapevine in Oregon and
Washington. Plant Disease, 89, 763–766.
- Naidu R., Rowhani A., Fuchs M., Golino D., Martelli G.,
2014. Grapevine leafroll: A complex viral disease affecting
a high-value fruit crop. Plant Disease, 98 (9), 1172-1185.
O’Donnell K., 1999. Plant pathogen diagnostics: Present
status and future developments. Potato Research, 42, 437–
447.
- Pietersen G., 2006. Spatio-temporal distribution dynamics
of grapevine leafroll disease in Western Cape vineyards.
Extended abstracts of the 15th Meeting of the International
Council for the Study of Virus and Virus-like diseases
of the Grapevine (ICVG) 3-7 April, Stellenbosch, South
Africa, 126-127.
- Pio Ribeiro G., Saldarelli P., Hong N., Xiang B.C.H., Zhang X.L., Wang G.P., 2004. First record of three grapevine viruses in the Chinese province of Sinkiang. Journal of Plant Pathology, 86, 26.
- Sharma A.M., Wang J., Duffy S., Zhang S., Wong M.K., Rashed A., 2011. Occurrence of Grapevine leafroll-associated virus complex in Napa Valley. PLoS ONE 6:e2622710.1371/journal.pone.0026227
- Teliz D., 1987. Field serological detection of viral antigens associated with grapevine leafroll disease. Plant Disease, 71, 704–709.
- Tsai C.W., Bosco D., Daane K.M, Almeida R.P.P., 2012. Effect of host plant tissue on the vector transmission of Grapevine leafroll-associated virus 3. Journal of Economic Entomology, 104, 1480–1485.
- Ward E., Foster S.J., Fraaije B.A., McCartney H.A., 2004. Plant pathogen diagnostics: immunological and nucleic acid-based approaches. Annals of Applied Biology, 145, 1–16.
- Zee F., Gonsalves D., Kim K.S., Pool R., Lee R.F., 1987. Cytopathology of leafroll diseases grapevine and the purification and serology of associated closteroviruslike particles. Phytopathology, 77, 1427-1434.