Bazı bitki ekstraktlarının in vitro koşullarda Botrytis cinerea Pers.’ya antifungal aktivitelerinin belirlenmesi

Bu çalışmada, meyve ve sebzelerde hastalığa neden olan Botrytis cinerea’ya karşı on bir bitkiden elde edilen ekstraktların etkinliğinin laboratuvar koşullarında araştırılması amaçlanmıştır. Çalışmalarda sarımsak (Allium sativum L.), lahana (Brassica oleracea var. capitata f. alba), kırmızılâhana (Brassica oleracea var. capitata f. rubra), rezene (Foeniculum vulgare Mill.), kişniş (Coriandrum sativum L.), zencefil (Zingiber officinale Roscoe), sumak (Rhus coriaria L.), kırmızı pancar (Beta vulgaris), zeytin (Olea europaea L.), ceviz (Juglans regia L.) ve yapışkan otu (Galium aparine L.) bitkilerinden elde edilen ekstraktlar kullanılmıştır. Bu bitkilerden etil alkol ile maserasyon yöntemiyle elde edilen ekstraktların petride B. cinerea’nın koloni gelişmesine karşı etkilerine bakılarak, ümitvar bulunan ekstraktların daha sonra alt doz denemeleri (1/1, 1/2, 1/3, 1/4, 1/5 oranlarında) yapılmıştır. Petri denemelerinde engelleyici etkisi tespit edilen ekstraktların fungusun spor çimlenmesi ve çim tüpü oluşumuna karşı etkileri de araştırılmıştır. Çalışma sonucunda zencefil, ceviz, kırmızılâhana ve sumak ekstraktlarının fungusun koloni gelişimini sırasıyla %69.52, %28.52, %20.96 ve %15.17 oranında engelleyerek antifungal aktiviteye sahip oldukları tespit edilmiştir. En yüksek etkiye sahip olan zencefil ekstraktının alt doz çalışmalarında da 1/4 dozuna kadar etkinliğin devam ettiği gözlenmiştir. Ayrıca, zencefil ekstraktının spor çimlenmesini %75.45 ve çim tüpü oluşumunu %84.91 oranında engellemesinin yanı sıra etmenin sporlarında çim tüplerinde kısalma ve deformasyona neden olarak patojene karşı en iyi etkiye sahip olduğu belirlenmiştir.

In vitro antifungal activity of some plant extracts against Botrytis cinerea Pers.

This study was aimed to investigate the efficacy of extracts obtained from elevenplants against Botrytis cinerea causing disease in fruit and vegetables underlaboratory conditions. The extracts obtained from plants of garlic (Allium sativumL.), cabbage (Brassica oleracea var capitata f. alba), red cabbage (Brassicaoleracea var capitata f. rubra), fennel (Foeniculum vulgare Mill.), coriander(Coriandrum sativum L .), g inger ( Zingiber officinale R oscoe), s umac ( Rhuscoriaria L.), red beet (Beta vulgaris), olive (Olea europaea L.), walnut (Juglansregia L.), and cleavers (Galium aparine L.) were used throughout the study.Based on the effects of the plant extracts obtained by maceration with ethylalcohol against the colony growth of B. cinerea in the petri dishes, potentialextracts were then subjected to sub-dose trials (1/1, 1/2, 1/3, 1/4, 1/5 ratios).The extracts, which inhibited colony growth of the pathogen in the petri disheswere also evaluated for their effects on spore germination and formation of thegerm tube. As a result, it was determined that ginger, walnut, red cabbage, andsumac extracts had antifungal activity by inhibiting the colony growth of fungusby 69.52%, 28.52%, 20.96%, and 15.17% respectively. It was observed that theginger extract having the highest effect continued its effectiveness up to 1/4dose. In addition, it was determined that ginger extract inhibited 75.45% of sporegermination and 84.91% of germ tube formation. It was also detected that it hasthe best effect against the pathogen by causing shortening and deformation inthe germ tubes in the spore of the pathogen.

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