Hakan BALBALOĞLU, Güldeniz KARADENİZ ÇAKMAK, İshak ÖZEL TEKİN, Şereften AÇIKGÖZ, Burak BAHADIR, Öge TAŞCILAR

Deneysel Pankreatitte Oksidatif Stres ve Lipid Peroksidasyon Durumunun Değerlendirilmesi

Amaç: Günümüzde, halen akut pankreatit patogenezi tartışma konusu olmaya devam etmektedir. Çalışmamız, taurokolata ile oluşturulan deneysel akut pankreatit modelinde oksidatif stres ve lipid peroksidasyon durumunu araştırmayı amaçlamaktadır. Araştırmamızda oksidatif stresin biyobelirteçi olarak, okside LDL'yi kullandık. Gereç ve Yöntemler: Çalışmamızda dört gruba ayrılmış yirmi altı Wistar albino sıçan kullandık. Sham gurubu, kontrol grubu (grup 1) ve sodyum taurokolata ile akut pankreatit oluşturulan deney grubu; 24. saatte (grup 2), 48. saatte (grup 3) ve 72. saatte (grup 4) sakrifiye edildi. Pankreas dokusu malondialdehit (MDA) düzeylerini lipid peroksidasyonunun bir göstergesi olarak değerlendirdik. İmmünofloresan boyama kullanarak pankreas dokularında ox-LDL varlığını belirledik ve pankreas dokularının histopatolojik analizi ile karşılaştırdık. Bulgular: Pankreas dokusunda MDA ve serum amilaz, ALT ve AST düzeylerinin grup 2, 3 ve 4'te anlamlı olarak yüksek olduğunu ve histopatolojik bulgularının da pankreatit ile uyumlu olduğunu gördük. Histopatolojik bulgular ile ox-LDL birikim düzeyini karşılaştırdık ve Grup 2, 3 ve 4'te pankreas dokusunda ox-LDL birikimi düzeyi ile pankreatit şiddeti arasında paralellik bulduk. Sonuç: Bu çalışma, taurokolata bağlı akut pankreatit modelinde pankreas dokusunda ox-LDL moleküllerinin birikimini göstermektedir. Ox-LDL dahil olmak üzere bu yan ürünlere göre, tedavi modalitelerinin akut pankreatitin lokal değil sistemik komplikasyonlarını iyileştirmede faydalı etkileri olabileceğini düşünüyoruz.

The Evaluation of Oxidative Stress and Lipid Peroxidation Status in Experimental Pancreatitis

Aim: Currently, acute pancreatitis pathogenesis remains a matter of debate. Our study aims to investigate the oxidative stress and lipid peroxidation state in taurocholate-induced acute pancreatitis, and we used oxidized LDL as a biomarker of oxidative stress. Material and Methods: We used twenty-six Wistar albino rats divided into four groups. Sham- operated rats, control group (group 1), and experimental group with sodium taurocholate-induced acute pancreatitis; were sacrificed at 24 hours (group 2), 48 hours (group 3), and 72 hours (group 4). We evaluated pancreatic tissue malondialdehyde (MDA) levels as an indicator of lipid peroxidation. Then, using the immunofluorescence staining, we determined the presence of ox-LDL in pancreatic tissues, which we used to compare the histopathological analysis of pancreatitis. Results: We found that MDA levels in pancreatic tissue and serum amylase, ALT, and AST were significantly higher in groups 2, 3, and 4. Histopathological findings of groups 2, 3, and 4 were compatible with pancreatitis, and we encountered the level of ox-LDL accumulation. We found acorrelation between the level of ox-LDL accumulation in pancreatic tissue and the severity of pancreatitis in groups 2, 3, and 4. Conclusion: This study demonstrates the accumulation of ox-LDL molecules in pancreatic tissue in a taurocholate-induced acute pancreatitis model. According to these byproducts, including ox-LDL, we conceive that treatment modalities might have beneficial effects in improving not only local but systemic complications of acute pancreatitis.

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