Saeid NAFISI, Mohammad Hossein ASGHARI, Mohammad Ali Mohammad NAZHADY

Comparative and mixture effect of cynodon dactylon, electromagnetic field and insulin on diabetic mouse

Amaç: Diabetes mellitusa bazı alternatif tedaviler bulmak için yeni araştırmalar devam etmektedir. Bu bağlamda bitkiler ilginç ilaçlardandır. Cynodon dactylon (C.d) yeni bir ilaç olarak düşünülmesi gereken bir potansiyel bitkidir. Diğer taraftan, Elektromanyetik Alanın (EMA) biyo-organizmalar üzerine etkisi daha net hale gelmektedir. Bu çalışmada diyabetik fareler üzerinde C.d, EMA ve insülinin etkisi araştırılmıştır. Materyal ve Metodlar: Sürekli hiperglisemi oluşturan ketamin (60 mg/Kg) ve xylazine (10 mg/Kg) ile diyabet oluşturuldu. Fareler 12 gruba ayrıldı 1) kontrol, 2) normal salin, 3 ve 4) 50 mg/Kg C.d, 5 ve 6) 100 mg/Kg C.d, 7) insülin, 8) insülin ve C.d, 9) EMA (110 KHz, 700±20 mG), 10) insülin ve EMA, 11) EMA artı C.d ve 12) insülin artı C.d ve EMA. C.d verilen gruplarda 5 ve 60 dakika sonra ve diğer gruplarda ise 5 dakika sonra glukometre seti ile kan glukoz seviyesi ölçüldü. Veriler ANOVA ile analiz edildi ve farklılıklar Tukey ve Bonferroni testleri ile karşılaştırıldı (p

Cynodon dactylon, elektromanyetik alan ve insülinin diyabetik fareler üzerinde karşılaştırmalı ve karma etkisi

Objective: New investigations are in progress to find some alternative treatments for diabetes mellitus. Herbs are some of the interesting medications in this regard. Cynodon dactylon (C.d) is a potential plant to be considered as a new medication. On the other hand, the effect of the Electromagnetic Field (EMF) on bio organisms is becoming clearer. In this study, the effect of C.d, EMF and insulin have been investigated on the diabetic mouse. Material and Methods: Diabetes was induced by a combination of ketamine (60 mg/Kg) and xylazine (10 mg/Kg) which induces a sustained hyperglycemia. Mice were divided into 12 groups: 1) control, 2) normal saline, 3 and 4) 50mg/Kg C.d, 5 and 6) 100 mg/Kg C.d, 7) insulin, 8) insulin and C.d, 9) EMF (110 KHz, 700±20 mG), 10) insulin and EMF, 11) EMF plus C.d and 12) insulin plus C.d and EMF. Blood glucose level was measured after 5 and 60 minutes in C.d administrated groups, and 5 minutes in the other groups by a glucometer set. The data were analyzed by ANOVA and different means were compared by Tukey and Bonferroni tests (p

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