Evaluation of food antioxidant activity by photostorage chemiluminescence
Evaluation of food antioxidant activity by photostorage chemiluminescence
A sensitive and simple method for measuring antioxidant activity was developed, based on photostoragechemiluminescence generated from photolyzed acridine and strong bases. This method was used to evaluate the antioxidant activity of food additives by measuring the inhibition of chemiluminescence at a gradient of antioxidant concentrations. Adipic, tartaric, citric, ascorbic, malic and fumaric acid were employed as antioxidants. The method is more suitable for lipophilic compounds.
___
- [1] R.J. Evans, G.S. Reynhout, In Food science and human nutrition, G. Charalambous, ed., Elsevier, Amsterdam, The Netherlands, 1992, pp27-42.
- [2] E.R. Sherwin, J. Am. Oil Chem. Soc. 45 (1968) 632A, 634A, 646A, 648A.
- [3] D. Coxon, Food Sci. Technol. Today, 1 (1987) 164.
- [4] J.I. Gy, J. Am. Oil Chem. Soc., 55 (1978) 539.
- [5] C. Pascual, C. Romay, J. Biolumin. Chemilum. 7 (1992) 123.
- [6] G.C. Yang, Trends Food Sci. Technol., 3 (1992) 15.
- [7] T. Miyazawa, H. Kunika, K. Fujimoto, Y. Endo, T. Kaneda, Lipids, 30 (1995) 1001.
- [8] M. Makinen, V. Piironen, A. Hopia, J. Chromatogr., A, 734 (1996) 221.
- [9] T.C. Christensen, G. Holmer, J. Food Sci., 61 (1996) 486.
- [10] G.A. Cash, G.A. George, J.P. Bartley, J. Sci. Food Agric. 43 (1998) 277.
- [11] O. Hirayama, M. Takagi, K. Hukumoto, S. Katoh, Anal. Biochem. 247 (1997) 237.
- [12] F. Visioli, C Galli, Anal. Biochem. 249 (1997) 244.
- [13] K. Papadopoulos, D. Dimotikali and J. Nikokavouras, J. Photochem. Photobiol. A: Chem., 103 (1997) 51.
- [14] K. Papadopoulos, J. Lignos, M. Stamatakis, D. Dimotikali, and J. Nikokavouras, J. Photochem. Photobiol. A: Chem., 115 (1998) 137.
- [15] K. Papadopoulos, T. Triantis, D. Dimotikali and J. Nikokavouras, J. Photochem. Photobiol. A: Chem., 131 (2000) 55.
- [16] K. Papadopoulos, T. Triantis, D. Dimotikali and J. Nikokavouras, Anal. Chim. Acta, 423 (2000) 239 and references therein.
- [17] G. Cao, H. Alessio, R.G. Cutler, Free Rad. Biol. Med. 14 (1993) 303.
- [18] G. Cao, C. Verdon, A. Wu, H. Wang, R. Prior, Clin. Chem. 41 (1995) 1738.
- [19] B. Haliwell, J.M.C. Gutteridge, Biochem. 219 (1984) 1.
- [20] K. Papadopoulos, T. Triantis, D. Dimotikali and J. Nikokavouras, J. Photochem. Photobiol. A: Chem., 124 (1999) 85.