Ulaş ACARÖZ, Recep KARA, Zeki GÜRLER, Ali SOYLU, Osman KÜÇÜKKURT

Taze Marul Örneklerinde Escherichia coli O157 ve Listeria monocytogenes Varlığının Belirlenmesi

Taze salatalar ve salata yapımında kullanılan sebzeler, çiğ tüketildiği için patojen bakteriler ile kontamine olması durumunda insansağlığı açısından ciddi riskler oluşturabilmektedir. Bu amaçla yapılan çalışmada, Afyonkarahisar ilinde tüketime sunulan taze marulörneklerinin mikrobiyolojik kalitesi incelendi. Çalışma kapsamında, market ve pazarlardan toplanan 70 adet taze marul örneğiEscherichia coli O157 ve Listeria monocytogenes varlığı yönünden analize alındı. E. coli O157 ve L. monocytogenes analizlerisırasıyla ISO 16654 ve ISO 11290-1’e göre gerçekleştirilmiştir. Analiz sonucunda, 70 adet taze marul örneğinin 2 (%2,86)’sinde E.coli O157 ve bir (%1,43) tane taze marul örneğinde ise L. monocytogenes, tespit edildi. Sonuç olarak, incelenen marul örneklerininpatojen bakterileri içerdiği ve mikrobiyolojik kalitesinin düşük seviyede olduğu belirlenmiştir. Isıl işlem olmadan tüketilen sebzelerdeE. coli O157 ve L. monocytogenes gibi patojen bakterilerin bulunması sağlık açısından ciddi bir risk oluşturmaktadır. Bu nedenle,mikrobiyolojik kaliteyi artırmak için etkili kontrol önlemlerinin uygulanması gerekmektedir.

Determination of the Presence of Escherichia coli O157 and Listeria monocytogenes in Fresh Lettuce Samples

Fresh salads and vegetables used for salad making may induce serious risks for human health if they are contaminated with pathogenic bacteria since they are consumed raw. In this study, microbiological quality of fresh lettuce samples which are offered for consumption in Afyonkarahisar province was investigated. For that aim, 70 fresh lettuce samples collected from markets and bazaars and analyzed for the presence of Escherichia coli O157 and Listeria monocytogenes. The analysis of E. coli O157 and L. monocytogenes was performed according to ISO 16654 and ISO 11290-1, respectively. The results of the present study showed that 2 (% 2.86) of 70 fresh lettuce samples were found to be positive for E. coli O157 while 1 (1.43%) fresh lettuce sample was positive for L. monocytogenes. In conclusion, the lettuce samples were found to contain pathogenic bacteria and their microbiological quality was low. The presence of pathogenic bacteria such as E. coli O157 and L. monocytogenes in vegetables consumed without heat treatment poses a serious health risk. Therefore, effective control measures should be implemented to increase microbiological quality.

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