Nanofiber Yapılı Aşırı Yükseltgenmiş Polipirol Modifiye Kalem Ucu Elektrotların Elektrokimyasal Epinefrin Tayininde Kullanımı

Bu çalışmada, kalem ucu elektrot yüzeyinde elektrokimyasal sentezlenerek aşırı yükseltgenen polipirol nanofiber elektrotlar hazırlanmışve (KUE/AYPP-NF) epinefrin tayinlerinde elektrokimyasal sensör olarak kullanımı araştırılmıştır. Modifiye elektrodun epinefrin(adrenalin) tayinlerine yönelik elektrokimyasal incelenmesinde ve analitik performanslarının belirlenmesinde dönüşümlü voltametri vediferansiyel puls voltametri (DPV) tekniği kullanılmıştır. Epinefrin tayinlerine pH’nın etkisi fosfat tamponu çözeltisinde KUE/AYPPNF kullanılarak incelenmiş ve en yüksek pik akımına pH 4,0 değerinde ulaşıldığı tespit edilmiştir. Nanofiber yapıda olmayan aşırıyükseltgeniş polipirol ile karşılaştırıldığında nanofiber yapının epinefrin yükseltgenmesini arttırdığı belirlenmiştir. DPV tekniğikullanılarak KUE/AYPP-NF için epinefrin yükseltgenmesine ait pik akımı 10-200 µM arasında 0,998 korelasyon katsayısı iledoğrusallık göstermektedir. Tayin sınırı (LOD) 2,2 µM olarak hesaplanmıştır (S/N=3). Sonuçlar KUE/AYPP-NF elektrotların epinefrintayinlerinde elektrokimyasal sensör olarak kullanılabileceğini göstermiştir.

Electrochemical Epinephrine Determination by Nanofiber Structured Overoxidized Polypyrrole Modified Pencil Graphite Electrodes

In this study, the overoxidized polypyrrole nanofiber on pencil graphite electrode (PGE/OPP-NF) surface were prepared and investigated to be used as an electrochemical sensor for determination of epinephrine (adrenaline,). Cyclic voltammetry and differential pulse voltammetry techniques were used for electrochemical investigation and determination of analytic performance for epinephrine detection, respectively. The effect of pH on epinephrine determination was investigated by using PGE/OPP-NF in phosphate buffer solution and the highest peak current was reached at pH 4.0. It was determined that the nanofiber structure increased the epinephrine oxidation compared to the non-nanofibre structured overoxidized polypyrrole. The peak current of epinephrine oxidation by PGE/OPPNF determined by using DPV technique is linear with a correlation coefficient of 0.998 in the range of 10-200 μM.The detection limit (LOD) of epinephrine is 2.2 μM. The results showed that PGE/OPP-NF electrodes could be used as electrochemical sensors for epinephrine determination.

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