Laktoperoksidaz Enziminin Sülfamat Türevleri Bileşikleri Üzerineİnhibisyon Profili

Sülfamat iskeleti içeren birçok doğal ürün ve ilaç etken maddelerinde bulunmaktadırlar. Son zamanlarda, sülfamatlar bileşiklerininbulundurdukları fonksiyonel gruplarından dolayı malzeme bilimcisi ve farmakolog tarafından daha fazla ilgi görmektedirler.Peroksidazlar (POD), metabolik fonksiyonlarından dolayı gıda ve ilaç endüstrisinde enzimatik reaksiyonlarda ve klinik teşhislerdeönemli kullanım alanında yer alırlar. Laktoperoksidaz (LPO, EC 1.11.1.7 ) peroksidaz ailesinin bir üyesidir. Bu enzim sütte, tükürükteve gözyaşında bulunan bir oksidoredüktaz olup, patojen mikroorganizmalara karşı yeni doğanların bağırsak sistemlerini ve meme bezlerini korumada önemli bir role sahip olmaktadır. Memeli sütlerinden edilen LPO enzimi, bakterilerin büyümesinin baskılanmasındave bakteri inhibisyonunun desteklenmesinde oldukça önemlidir. Sığır LPO’sunun bakteriyal büyümeyi inhibe etmesi, H2O2 vetiyosiyanat içeren peroksidaz sistemine atfedilir. Bu sistemin antimikrobiyal etkisi sütte doğal olarak oluşmuştur. LPO enzimi üzerineyapılan antibakteriyal çalışmalarda LPO-tiyosiyanat ve peroksit sisteminin patajonik bakterilerde önemli derecede inhibisyona sebepolduğu tespit edilmiştir. LPO’nun birçok uygulama alanı vardır. Peroksidazlar hem gıdalarda hem de farmakolojik uygulamalardakoruyucu olarak kullanılır ve süt işleme tesislerinde nakil esnasında sütün muhafazası amacıyla süt endüstrisinde kullanılmaktadır. Buçalışmanın amacı, LPO enzimi üzerine Metil benzoilsülfamat, Metil (2-bromobenzoil) sülfamat, Metil (3-fenilpropanoil) sülfamat,Metil (1-naphthoil) sülfamat, Metil (2-metilbenzoil) sülfamat, Metil (2-iyodobenzoil) sülfamat, Metil (2-fenilbutanoil) sülfamat, Metil(4-isopropilbenzoil) sülfamat, Metil (4-metoksibenzoil) sülfamat ve Metil (isoquinoline-1-karbonil) sülfamat bileşilklerinin in vitroetkilerini belirlemektir. Bu sülfamat türevi bileşiklerinin LPO enzimi üzerindeki inhibisyon etkisini belirlemek için, enzim aktiviteleriölçülerek her bir inhibitör için Lineweaver-Burk grafikleri çizildi; Ki sabiti ve inhibisyon tipleri bu çizilen grafiklerden bulnunarakhesaplanıldı. Ki değerleri sırasıyla Metil benzoilsülfamat 0,70 μM, Metil (2-bromobenzoil) sülfamat 0,025 μM, Metil (3-fenilpropanoil) sülfamat 0,018 μM, Metil (1-naphthoil) sülfamat 0,047 μM, Metil (2-metilbenzoil) sülfamat 0,043 μM, Metil (2-iyodobenzoil) sülfamat0,19 μM, Metil (2-fenilbutanoil) sülfamat 0,39 μM, Metil (4-isopropilbenzoil) sülfamat 0,42 μM, Metil (4-metoksibenzoil) sülfamat0,078 μM, Metil (isoquinoline-1-karbonil) sülfamat 0,075 μM olarak belirlendi. Metil (4-isopropilbenzoil)sülfamat bileşiği yarışmasızinhibisyon ve diğer maddeler yarışmalı inhibisyon gösterdiği kaydedildi. Metil (2-metilbenzoil)sülfamat bileşiği ise en etkili inhibitörözelliğini yarışmalı inhibisyon tipi LPO enzimi üzerine 0.018 ± 0.024 μM Ki değeri ile göstermiştir.

Inhibition Profile of Lactoperoxidase Enzyme on Sulfamate Derivatives

They are found in many natural products and active ingredients that contain a sulfamate skeleton. Recently, due to the functional groupsof sulfamates compounds, they have received more attention from the material scientist and pharmacologist. Due to their metabolicfunctions, peroxidases (POD) are important in enzymatic reactions and clinical diagnoses in the food and pharmaceutical industry.Lactoperoxidase (LPO, EC 1.11.1.7) is a member of the peroxidase family. This enzyme is an oxidoreductase found in milk, saliva and tears and has an important role in protecting the gut systems and mammary glands of newborns against pathogenic microorganisms.The LPO enzyme from mammalian milk is very important in suppressing the growth of bacteria and promoting bacterial inhibition.Inhibition of bacterial growth of bovine LPO is attributed to the peroxidase system containing H2O2 and thiocyanate. The antimicrobialeffect of this system occurs naturally in milk. In antibacterial studies on LPO enzyme, LPO-thiocyanate and peroxide system has beenfound to cause a significant inhibition of pathogenic bacteria. LPO has many application areas. Peroxidases can be used as preservativesin both food and pharmacological applications, and are used in the milk industry for milk preservation during transport in milkprocessing plants. The aim of this study is to determine the in vitro effects of Methyl benzoylsulfamate, Methyl (2-bromobenzoyl)sulfamate, Methyl (3-phenylpropanoyl) sulfamate, Methyl (1-naphthoyl) sulfamate, Methyl (2-methylbenzoyl) sulfamate, Methyl (2- iodobenzoyl) sulfamate, Methyl (2-phenyl), Methyl (4-isopropylbenzoyl) sulfamate, Methyl (4-methoxybenzoyl) sulfamate and Methyl(isoquinoline-1-carbonyl) sulfamate compounds on LPO enzyme. To determine the inhibition effect of these sulfamate derivativecompounds on the LPO enzyme, Lineweaver-Burk plots were drawn for each inhibitor by measuring enzyme activities; Ki constant andinhibition types were calculated from these plotted graphs. Ki values were determined as Methyl benzoylsulfamate 0.70 μM, Methyl(2-bromobenzoyl) sulfamate 0.025 μM, Methyl (3-phenylpropanoyl) sulfamate 0.018 μM, Methyl (1-naphthoyl) sulfamate 0.047 μM,Methyl (2-methylbenzoyl) sulfamate 0.043 μM, Methyl (2-methylbenzoyl) sulfamate 0.043 μM -Iodobenzoyl) sulfamate 0.19 μM,Methyl (2-phenylbutanoyl) sulfamate 0.49 μM, Methyl (4-isopropylbenzoyl) sulfamate 0.42 μM, Methyl (4-methoxybenzoyl) sulfamate0.078 μM, Methyl (isoquinoline-1-carbonyl ) sulfamate 0.075 μM, respectively. Methyl (4-isopropylbenzoyl) sulfamate compound wasnoted to exhibit non-competitive inhibition and other substances showed competitive inhibition. Methyl (2-methylbenzoyl) sulfamatecompound, on the other hand, showed its most effective inhibitory feature on competitive competitive inhibition type LPO enzyme with a value of Ki 0.018 ± 0.024 μM.

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