A Turn OFF Fluorescent Probe For Selective Detection Of $Hg^{2+}$ Ions

Bu çalışmada imidazol içeren yeni bir floresan sensör sentezlenmiş ve sentezlenen sensörün karakterizasyonu ${}^{1}H$NMR, ${}^{13}C$-NMR spektroskopisi, FT-IR spektroskopisi ve elemental analiz cihazı kullanılarak yapılmıştır. Hazırlanan sensör etanol içerisinde civa iyonlarına hem hızlı hem de seçici olarak floresanı söndürerek cevap vermiştir. Sentezlenen 2-((4-(1H-Fenantreimidazol [9,10-d] imidazol-2-)benziliden)amino) fenol (PENIM) and $Hg^{+}$ iyonları arasında kompleksleşme gerçekleştirilmiş ve bu komplesleşme JOB metodu kullanılarak da kompleksleşme oranı teorik olarak hesaplanmıştır. Sentezlenen probun deteksiyon limiti 3σ/k formülü kullanılarak 2.1 nM olarak tespit edilmiştir. Ayrıca, elde edilen civa (II)-PENIM, merkapto molekülü içeren sistein amino asidine karşı tersinir olarak cevap vermiştir. PENIM molekülünün civa iyonlarına karşı cevap vermesinin sonucu olarak floresan şiddetinin artırılması ve söndürülmesi ile ilgili çalışma, yoğun fonksiyonel hesaplama(DFT) yapılarak teorik olarak desteklenmiştir.

A Turn OFF Fluorescent Probe For Selective Detection Of $Hg^{2+}$ Ions

In this study, we prepared a novel fluorescent chemosensor containing an imidazole molecule and the chemosensor characterizedutilizing ${}^{1}H$ NMR, ${}^{13}C$-NMR spectroscopy, FT-IR spectrometer and elemental analyzer. Prepared sensor was utilized as an effectivelyselective and a fastly responsive chemical fluorescent sensor for ‘’turn off’’ determination of mercury (II) ions in EtOH. A clearcomplex between 2-((4-(1H-phenanthro[9,10-d] imidazol-2-yl)benzylidene)amino) phenol (PENIM) and $Hg^{+}$ ions was determinedand calculated employing the Job’s method and also the limit of detection value was found to be 2.1 nM on the basis of 3σ/k.Furthermore, the sensor-$Hg^{2+}$ displayed and reversible property for mercapto containing cysteine molecules. Also, the fluorescenceenhancement and quenching studies were supported by computational experiments based on the density functional theory (DFT)calculations.

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