H. Hüseyin HADİMLİ, Zafer SAYIN, Osman ERGANİŞ, Kürşat KAV

Subklinik mastitisli süt ineklerinden izole edilen katalaz negatif Gram pozitif kokların identifikasyonu ve antibiyotiklere duyarlılıkları

Amaç: Bu çalışmada, subklinik mastitisli süt ineklerinin süt örneklerinden izole edilen katalaz negatif Gram pozitif kokların (KNGP) identifikasyonu ve etkenlerin antibiyotiklere duyarlılıklarını belirlenmesi amaçlandı. Gereç ve Yöntem: Subklinik mastitisli süt ineklerinden toplam 104 KNGP kok izole edildi. İzolatlar koloni morfolojisi ve hemoliz özelliklerine göre seçildi ve VİTEC 2 ile identifiye edildi.Bulgular: İzolatların 32’si Enterococcus faecalis (%30.76), 21’i Aerococcus viridans (%20.19), 18’i E. faecium (%17.30), 13’ü Streptococcus agalactiae (%12.50), 11’i S. uberis (%10,57), 3’ü Lactococcus garviae/lactis ssp. lactis (%2,88), 2’si L. lactis ssp. latis (%1.92) ve 1’i L. garviae (%0.96) olarak tanımlandı. Üç izolat (%2,88) ise identifiye edilemedi. KNGP kokların antimikrobiyal ajanlara karşı duyarlılıklarının farklı olduğu tespit edildi. Öneri: Bu çalışma, subklinik mastitisli süt ineklerinden izole edilen KNGP kokların doğru identifikasyonlarının yapılması gerektiğini gösterdi.

Anahtar Kelimeler:

Gram pozitif kok, mastitis, süt ineği

Identification and antibiotic susceptibility of catalase negative Gram positive cocci isolated from dairy cows with subclinical mastitis

Aim: This study was aimed to identify of catalase negative Gram positive cocci (CNGP) isolated from milk samples of dairy cows with subclinical mastitis and to determine susceptibility of microorganisms to antibiotics. Materials and Method: Totally, 104 CNGP cocci were isolated from dairy cows with subclinical mastitis. Isolates were selected according to coloni morphological and haemolysis properties and were identified by VITEC 2. Results: Out of isolates, 32 (30.76%) were identified as Enterococcus faecalis, 21 (20.19%) Aerococcus viridans, 18 (17.30%) E. faecium, 13 (12.50%) Streptococcus agalactiae, 11 (10.57%) S. uberis, 3 (2.88%) Lactococcus garviae/lactis ssp. lactis, 2 (1.92%) L. lactis ssp. latis and 1 (0.96%) L. garviae. Three isolates (2.88%) could not be identified. The susceptibilities of CNGP cocci were determined to be different to antimicrobial agents. Conclusion: This study shown that identification of CNGP cocci isolated from dairy cows with subclinical mastitis should be performed accordingly.

Keywords:

Gram positive cocci, mastitis, dairy cow,

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