Marcela Capcarova, Adriana Kolesarova, Anna Kalafova, Branislav Galik, Milan Simko, Miroslav Juracek, Robert Toman

Rat diyetlerine arı poleni ilavesinin antioksidan durum üzerine etkisi

Amaç: Mevcut araştırmada ratların oksidatif durum parametreleri üzerine gıda desteği olaral arı poleni ilavesinin etkisi incelendi. Gereç ve Yöntem: Yetişkin Wistar ratlar 3 gruba ayrıldı: Birinci grup kontrol (C) olarak ayrılırken, diğer iki grup deneme grupları (E1, E2) olarak ayrıldı. Deneme gruplarından E1 grubu diyetine 300 mg/kg arı poleni ilavesi yapılırken, ikinci E2 grubuna 500 mg/kg dozunda ilave yapılarak 90 gün beslendi. Arı poleni ilavesi yapılmayan grup ise kontrol grubu olarak değerlendirildi. Albümin, bilirubin, demir, total antioksidan durum ve süperoksid dismutaz düzeyleri spektrofotometri ile belirlendi. Bulgular: Ratların diyetlerine 500 mg/kg dozunda arı poleni ilavesinin albümin ve total antioksidan durum düzeylerinin istatistiki olarak (P

Anahtar Kelimeler:

Arı poleni, antioksidanlar, kan

The role of dietary bee pollen in antioxidant potential in rats

Aim: The present study was designed to determinate the effect of bee pollen as a feed additive on the selected parameters of antioxidant status of rats. Material and Methods: Adult Wistar rats were randomly divided into 3 groups: One control group (C) and two experimental groups (E1, E2). Experimental groups received dietary inclusion of collected bee pollen at 300 mg/kg in E1 group and 500 mg/kg in E2 group for 90 days. The group

Keywords:

Bee pollen, antioxidants, blood,

PDF

___

Ahn MR, Kunimasa K, Kumazawa S, Nakayama T, Kaji K, Uto Y, Hori H, Nagasawa H, Ohta T, 2009. Correlation between antiangiogenic activity and antioxidant activity of various components from propolis. Mol Nutr Food Res, 53, 643-651
Akkol EK, Orhan DD, Gürbüz I, Yesilada E, 2010. In vivo activity assessment of a “honey-bee pollen mix” formulation. Pharm Biol, 48, 253-259.
Aliyazicioglu Y, Deger O, Ovali E, Barlak Y, Hosver I, Tekelioglu Y, Karahan SC, 2005. Effects of Turkish pollen and propolis extracts on respiratory burst for K-562 cell lines. International Immunopharmacol, 5, 1652-1657.
Attia Y A, Al-Hanoun A, Bovera F, 2011a. Effect of different levels of bee pollen on performance and blood profile of New Zealand White bucks and growth performance of their offspring during summer and winter months. J Anim Physiol Anim Nutr (Berl), 95, 17-26.
Attia YA, Al-Hanoun A, El-Din AE, Bovera F, Shewika YE, 2011b. Effect of bee pollen levels on productive, reproductive and blood traits of NZW rabbits. J Anim Physiol Anim Nutr (Berl), 95, 294-303.
Bankova V, 2009. Chemical diversity of propolis makes it a valuable source of new biologically active compounds. J ApiProduct ApiMed Sci, 1, 23-28. DOI: 10.3896/IBRA.4.01.2.01
Bell RR, Thormber EJ, Seet JLL, Groves MT, Ho NP, Bell DT, 1983. Composition and protein quality of honeybee collected pollen of Eucalyptus marginate and Eucalyptus calophylla. J Nutr, 113, 2479-2484.
Bonvehí SJ, Torrentó SM, Lorente CE, 2001. Evaluation of polyphenolic and flavonoid compounds in honeybeecollected pollen produced in Spain. J Agr Food Chem, 49, 1848-1853.
Buck AC, Cox R, Rees RW, Ebeling L, John A, 1990. Treatment of outflow tract obstruction due to benign prostatic hyperplasi with the pollen extract, cernilton. A doubleblind, placebo-controlled study. Brit J Urol, 66, 398-404.
Capcarova M, Slamecka J, Abbas K, Kolesarova A, Kalafova A, Valent M, Filipejova T, Chrastinova L, Ondruska L, Massanyi P, 2011. Effects of dietary inclusion of Rhus coriaria on internal milieu of rabbits. J Anim Physiol Anim Nutr (Berl), 96, 459-465.
Echigo T, Yanami K, 1986. Studies on chemical composition of pollen loads. Honeybee Sci, 7, 97-100.
Eraslan G, Kanbur M, Silici S, 2009a. Effect of carbaryl on some biochemical changes in rats: the ameliorative effect of bee pollen. Food Cem Toxicol, 47, 86-91.
Eraslan G, Kanbur M, Silici S, Liman BC, Altmordulu S, Sarica ZS, 2009b. Evaluation of protective effect of bee pollen against propoxur toxicity in rat. Ecotox Environ Safe, 72, 931-937.
Fatrcova-Sramkova K, Nozkova J, Mariassyova M, Kacaniova M, Dudrikova E, 2008. Content of polyphenols and antiradical activity of bee pollen. Chem Listy, 102, 265-267.
Fu YY, Kang KJ, Ahn JM, Kim HR, Na KY, Chae DW, Kim S, Chin HJ, 2010. Hyperbilirubinemia reduces the streptozotocininduced pancreatic damage through attenuating the oxidative stress in the Gunn rat. Tohoku J Exp Med, 222, 265- 273.
Ishikawa Y, Tokura T, Ushio H, Niyonsaba F, Yamamoto Y, Tadokoro T, Ogawa H, Okumura K, 2009. Lipid-soluble components of honeybee-collected pollen exert antiallergic effect by inhibiting IgE-mediated mast cell activation in vivo. Phytoth Res, 23, 1581-1586.
Izuta H, Narahara Y, Shimazawa M, Mishima S, Kondo S, Hara H, 2009. 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of bee products and their constituents determined by ESR. Biol Pharm Bull, 32, 1947-1951.
Jackie T, Haleagrahara N, Chakravarthi S, 2011. Antioxidant effects of Etlingera elatior flower extract against lead acetate-induced perturbations in free radical scavenging enzymes and lipid peroxidation in rats. BMC Res Notes, 4, doi:10.1186/1756-0500-4-67.
Kacaniova M, Fatrcova-Sramkova K, Nozkova J, Melich M, Kadasi-Horakova M, Knazovicka V, Felsöciova S, Kunova S, Mariassyova M, 2011. Antiradical activity of natural honeys and antifungal effect against Penicillium genera. J Environ Sci Health, Part B, 46, 92-96.
Kanner J, Frankel E, Granit R, German B, Kinsella JE, 1994. Natural antioxidants in grapes and wines. J Agr Food Chem, 42, 64-69.
Leja M, Mareczek A, Wyzgolik G, Klepacz-Baniak J, Czekonska K, 2007. Antioxidative properties of bee pollen in selected plant species. Food Chem, 100, 237-240.
Marinova MD, Tchorbanov BP, 2010. Preparation of antioxidant enzymatic hydrolysates from honeybee-collected pollen using plant enzymes. Enzyme Res, ID 415949, doi:10.4061/2010/415949
Martin-Munoz MF, Bartolome B, Caminoa M, Bobolea I, Ara MC, Quirce S, 2010. Bee pollen: a dangerous food for allergic children. Identification of responsible allergens. Allergol Immunopathol, 38, 263-265.
Maruyama H, Sakamoto T, Araki Y, Hara H, 2010. Antiinflammatory effect of bee pollen ethanol extract from Cistus sp. of Spanish on carrageenan-induced rat hind paw edema. BMC Complement Altern Med, 10, 30, doi: 10.1186/1472-6882-10-30.
Missima F, Pagliarone AC, Orsatti CL, Sforcin JM, 2009. The effect of propolis on pro-inflammatory cytokines produced by melanoma-bearing mice submitted to chronic stress. J ApiProduct ApiMed Sci, 1, 11-15.
Moreira LL, Dias LG, Pereira JA, Estevinho L, 2008. Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal. Food Chem Toxicol, 46, 3482- 3485. Münstedt K, Bogdanov S, 2009. Bee products and their potential use in modern medicine. J ApiProduct ApiMed Sci, 1, 57-63.
Naik DG, Vaidya HS, Behera BC, 2009. Antioxidant properties of Indian propolis. J ApiProduct ApiMed Sci, 1, 110-120.
Nakajima Y, Tsuruma K, Shimazawa M, Mishima S, Hideaki H, 2009. Comparison of bee products based on assays of antioxidant capacities. BMC Complemen Altern Med, 9, doi:10.1186/1472-6882-9-4. http://www.biomedcentral. com/1472-6882/9/4
Petruska P, Tusimova E, Kalafova A, Hascik P, Kolesarova A, Capcarova M, 2012. Effect of propolis in chicken diet on selected parameters of mineral profile. J Microbiol Biotechnol Food Sci, 1, 593-600.
Saric A, Balog T, Sobocanes S, Kusic B, Sverko V, Rusak G, Likic S, Bubalo D, Pinto B, Reali D, Marotti T, 2009. Antioxidant effects of flavonoid from Croatian Cystus incanus L. rich bee pollen. Food Chem Toxicol, 47, 547-554.
Smith MW, Shenoy KB, Debnam ES, Dashwood MR, Churchill LJ, Srai SK, 2002. Divalent metal inhibition of non-haem uptake across the rat duodenal brush border membrane. Br J Nutr, 8, 51-56.
Srigiridhar K, Nair KM, 2000. Supplementation with alphatocopherol or a combination of alpha-tocopherol and ascorbic acid protects the gastrointestinal tract of irondeficient rats against iron-induced oxidative damage during iron repletion. Br J Nutr, 84, 165-173.
Stohs SJ, Bagchi D, 1995. Oxidative mechanisms in the toxicity of metal ions. Free Radical Biol Med, 18, 321-336. Stocker R, 2004. Antioxidant activities of bile pigments. Antioxid Redox Signal, 6, 841-849.
Valko M, Morris H, Cronin MTD, 2005. Metals, toxicity and oxidative stress. Curr Med Chem, 12, 1161-1208.
Vinson JA, Hontz BA, 1995. Phenol antioxidant index: comparative antioxidant effectiveness of red and white wines. J Agr Food Chem, 43, 401-403.
Xu X, Sun L, Dong J, Zhang H, 2009. Breaking the cells of rape bee pollen and consecutive extraction of functional oil with supercritical carbon oxide. Innov Food Sci Emerg Technol, 10, 42-46.