Düzeltme: Çörek Otu Tohumu Esansiyel Yağının Antioksidan Etkinliğinin in vitro Yöntemlerle Araştırılması

Bu çalışmada timokinon benzeri pekçok biyoktif madde içeren çörek otu (Nigella sativa L. ) tohumu esansiyel yağının antioksidan özellikleri çeşitli in vitro yöntemlerle araştırıldı. Çörek otu tohumu esansiyel yağı için toplam fenolik madde tayini, DPPH radikali giderme aktivitesinin tayini, linoleik asit sisteminde antioksidan aktivite, Fe+2 iyonlarını şelatlama aktivitesi, süperoksit radikali giderme aktivitesi tayini ve indirgeme kapasitesi belirlendi. Buna göre çörek otu tohumu esansiyel yağında toplam fenolik madde miktarı 30.5 μg/ml, DPPH radikali giderme aktivitesi %25.10 inhibisyonda, linoleik asit sisteminde ferrik tiyosiyanat metodu ile belirlenen antioksidan aktivite %23 inhibisyonda, Fe+2 iyonlarını şelatlama aktivitesi %52.33, süperoksit radikali giderme aktivitesi %20.63 inhibisyonda ve indirgeme kapasitesi ise 0.66 absorbans olarak belirlendi. Sonuç olarak bu in vitro antioksidan aktiviteleri gözönüne alındığında çörek otu tohumu esansiyel yağının gıda, kozmetik, farmasötik ve hayvan besleme alanında ucuz ve doğal alternatif bir antioksidan olarak değerlendirilebileceği öngörülmektedir.

Anahtar Kelimeler:

Antioksidan aktivite, Çörek otu tohumu esansiyel yağı, Toplam fenolik madde

Düzeltme: Research on the Antioxidant Efficiacy of Black Seed Essential Oil Using by in vitro Method

Medicinal plants such as black seed have antioxidant properties due to their essential oils. In this study, antioxidantproperty of particulary black seed (Nigella sativa L.) essential oil containing many bioactive substances such asthymoquinone, dithymoquinone, timol, nigellon, carvacrol was investigated in vitro with various methods. Totalphenolic substances, DPPH radical removal activity, antioxidant activity with ferric thiocyanate method in linoleic acidsystem, chelation activity of Fe+2 ions, superoxide radical removal activity and reduction capacity were determinedby spectrophotometric methods for black seed essential oil. Accordingly, total phenolic substances was 30.5 μg/mL,1,1-diphenyl-2-picrylhydrazyl (DPPH) radical removal activity was 25.10% inhibition, linoleic acid reduction activity was23% inhibition, Fe+2 ions reduction activity was 52.33% inhibition, superoxide radical removal activity was 20.63%inhibition and reduction capacity was 0.66 absorbance for Nigella sativa seed essential oil. In conclusion, consideringtheir in vitro antioxidant activities, it can be stated that Nigella sativa L. seed essential oil can be evaluated as anatural and cheap alternative antioxidant in different fields such as human and veterinary medicine.

Keywords:

: Antioxidant activity, Black seed essential oil, Total phenolic substance.,

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