Farzane PORDEL, Arash HOSSEİN POUR, Ramazan ÇAKMAKÇI

Buğdayda (Triticum aestivum L.) Erken Alüminyum Stresine Karşı Bakteri Uygulamalarının Etkileri

Buğday; taşınması, tarımı, saklanma ve işlenmesinin oldukça kolay olması; kışlık ve yazlık üretilebilmesi ve besin değerinin yüksek olması gibi nedenlerle, dünyada ve Türkiye’de temel besin maddelerinden birini oluşturmaktadır. Bu çalışmada buğdayda (Triticum aestivum L. cv Kırık) erken aluminyum stresine karşı bakteri uygulamalarının etkileri buğday çimlenme parametreleriyle araştırılmıştır. Araştırma tesadüf parsellerinde 4 farklı alüminyum chloride konsantrsyonu (0, 7,5, 15, 22,5 ve 30 mM) × 11 (farklı bakteri) faktöriyel deneme desenine göre 4 tekrarlı olarak yürütülmüştür. Sterilize edilen buğday tohumları 24 saat sıvı besi yerinde geliştirilmiş farklı bakteri türleri içerisinde tohum kodlaması yapılmıştır. Ardından tohumlara alüminyum chloride (AlCl3) uygulanarak karanlık fotoperyot ortamında 20°C’de 10 gün süreyle kağıt üzerinde çimlendirilmiştir. Bu çalışma sonunda çimlenme oranı (ÇO), çimlenme hızı katsayısı (ÇHK), ortalama çimlenme zamanı (OÇZ), çimlenme gücü indeksi, çim gücü indeksi (ÇGİ), toplam embriyonal kök uzunluğu ve sürgün uzunluğu parametreleri ölçülmüştür. AlCl3’in konsantrasyonu artıkça tüm bakteri uygulamalarında çimlenme oranı, çimlenme hızı kat sayısı, çimlenme gücü indeksi, kök uzunluğu ve sürgün uzunluğu çok önemli derecede azalmış buna karşın ortalama çimlenme zamanı uzamıştır. Farklı bakteri uygulamalarının buğdayda alüminyum stresine karşı verdiği tepkiler değerlendirildiğinde, Pseudomonas fluorescens bakterisi aşılamasının, diğer uygulamalara göre göre daha iyi sonuç verdiği ve test edilen çimlenme karakterleri bakımdan alüminyumun olumsuz etkisinin azaltılmasında etkili olduğu belirlenmiştir.

Anahtar Kelimeler:

Alüminyum, Bakteri, Buğday, Çimlenme, Stres

Effects of Differential Bacteria Application on Early Aluminum Stress in Wheat (Triticum aestivum L.)

Wheat, transport, cultivation, storage and processing are very easy; winter and summer produce, and high nutritional value of reasons such as; in the world and in Turkey constitutes one of the essential nutrients. In this study, the effects of different bacterial applications on different germination parameters of aluminum stress were aimed, this study was conducted in 4 replicates according to the completely randomized factorial design. As the plant material, the seeds of the wheat (Triticum aestivum L.cv. Kırik) were used. Sterilized wheat seeds seed coding was done in 11 different bacterial species developed in liquid medium for 24 hours. The seeds were then germinated on paper in dark photoperiod formed with aluminum chloride (AlCl3) for 10 days at 20 ° C. At the end of this study, the germination rate (GR), germination rate coefficient (CVG), mean germination time (MGT), germination vigor index (GVI), total embryonic root length and shoot length parameters were measured. The germination rate, germination vigor index, germination rate, root length and shoot length decreased significantly in all bacterial applications with different concentration of AlCl3, whereas mean germination time was prolonged. When the responses of different bacterial inoculations against aluminum stress in wheat were evaluated, inoculation with Pseudomonas fluorescens was found to be better than other applications and it was found to be effective in reducing the negative effect of aluminum in terms of tested germination characteristics.

Keywords:

Aluminum, Bacteria, Wheat, Germination, Stress,

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