Zekeriya ÖZÜDOĞRU, Hülya BALKAYA, Hülya KARA, Derviş ÖZDEMİR, Emre ERBAŞ

Saksağan (Pica pica)’da Pankreas Dokusunun Morfolojik Yapısının İncelenmesi

Pankreas, endokrin ve ekzokrin salgı özelliğine sahip, hayvan türleri arasında beslenme alışkanlıklarına bağlı olarak bazı karakteristik ve fonksiyonel farklılıklar gösteren bez yapısında bir organdır. Bu çalışmada, saksağan (Pica pica) pankreas dokusunun morfolojik ve fonksiyonel özelliklerinin makro-anatomik ve histolojik yöntemlerle araştırılması amaçlanmıştır. Çalışmada, saksağanlardan elde edilen pankreas diseke edilerek %10'luk tamponlu formaldehit solüsyonuna alındı. Tespit edilen pankreas dokuları alkol ve xylol serilerinden geçirilerek dehidratasyon ve şeffaflaştırma işlemlerinden sonra parafin bloklara gömülerek 5 µm kalınlığında kesitler alındı. Alınan seri kesitlere histolojik ve immonohistokimyasal boyamalar uygulanarak endokrin ve ekzokrin pankreas alanları incelendi. Ekzokrin pankreas dokusunun zengin seröz asinuslardan oluştuğu, damarlara yakın olarak yerleşmiş olan endokrin adacıklarının ise tüm pankreas dokusunun %1-2'sini oluşturduğu belirlendi. Ayrıca, endokrin adacıkların da alfa, beta ve miks adacıklardan oluştuğu görüldü. Sonuç olarak; endokrin ve ekzokrin pankreas dokusunun beslenmeye bağlı olarak bazı karakteristik ve fonksiyonel farklılıklar gösterebileceği tespit edildi

Morphologic Structure Analysis of Magpie (Pica pica)’s Pancreas Tissue

Pancreas is a gland, consisted of endocrine and exocrine areas and shows some characteristic and functional differences between animal species. In this study, it was aimed to investigate the macro-anatomical and histologic features of magpie (Pica pica)’s pancreas by histologic methods. In the study, magpies pancreas lobes obtained were fixed in 10% buffered formaldehyde solution. Fixed tissues were dehydrated in alcohol series, cleaned in xylene and embedded in paraffin blocks and paraffin block were cut and obtained 5-µm thickness of sections. The section was histologically and immunohistochemically stained and endocrine and exocrine areas were examined. Exocrine pancreas consisted of numerous serous acinus and endocrine pancreas were 1-2% ratio of total pancreas and located in especially near the blood vessels. Also, it was observed that endocrine islets were consisted of alpha, beta and mix islets. The results of this study revealed that endocrine and exocrine pancreas could show the some characteristic and functional differences depending on feeding habits

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