Hüseyin Serkan EROL, Nergis ULAŞ, Mustafa Sinan AKTAŞ, Cihan GÜR, Harun POLAT, Seçkin ÖZKANLAR, İsmail ABİDİN

BALB/C, BDNF Homozigot (+/+) ve BDNF Heterozigot Transgenik (+/-) Farelerde Glukoz, Lipid ve Protein Parametrelerinin Karşılaştırılması

Beyin Kaynaklı Nörotrofik Faktör (BDNF, Brain Derived Neurotrophic Factor) nöronlardan salgılanarak nöronların gelişimi ve olgunlaşmasında rol oynayan bir faktördür. BDNF proteinini kodlayan iki alelden birinden yoksun olan transgenik fare modelinde pek çok beyin bölgesinde BDNF ekspresyonu %50 oranında azalmıştır (BDNF heterozigot transgenik (+/-)). BDNF proteininin nörolojik etkilerinin yanı sıra metabolizma üzerine etkilerinin de araştırılması gerekmektedir. Bu çalışmada BALB/C, BDNF homozigot (+/+) ve BDNF heterozigot transgenik (+/-) farelerde glukoz, lipid ve protein parametrelerinin karşılaştırılması amaçlandı. Araştırmada 10 adet BALB/C ırkı ergin fare ve vahşi tip (WT, wild type) ırktan 9 adet WT BDNF homozigot normal (+/+) ve 4 adet WT BDNF heterozigot transgenik (+/-) ergin fare kullanıldı. Farelerin BDNF heterozigot transgenik (+/-) ve homozigot normal (+/+) oldukları RT-PCR ile belirlendi. Gruplarda elde edilen bulgular karşılaştırıldığında, BALB/C grubundaki hayvanlara kıyasla WT gruplarındaki hayvanlarda glukoz ve lipid düzeylerinin önemli derecede düşük olduğu ve total protein düzeylerinin ise yüksek olduğu tespit edildi. WT gruplarındaki BDNF homozigot (+/+) ve BDNF heterozigot transgenik (+/-) fareler arasında bu parametreler arasında önemli bir farklılığın olmadığı belirlendi. Sağlıklı normal farelerde BDNF proteinin eksikliğinin glukoz, lipid ve protein düzeylerini önemli derecede etkilemediği anlaşıldı. BDNF'nin metabolizma üzerine etkileri ile ilgili daha fazla çalışma yapılması gerekmektedir. Özellikle metabolik hastalıklar sırasında BDNF'nin rolü üzerine çalışmalar metabolik etkilerin daha iyi anlaşılmasını sağlayacaktır

Comparison of Glucose, Lipid and Protein Parameters in BALB/C, BDNF Homozygous (+/+) and BDNF Heterozygous Transgenic (+/-) Mice

Brain Derived Neurotrophic Factor (BDNF) is a factor that released by neurons and has role in the development and growth of neurons. Expression of BDNF is reduced by 50 % ratio in the most of the brain region of transgenic mouse model (BDNF heterozygous transgenic (+/-)), which is deprived of one of the two alleles coding BDNF protein. Besides, neurologic effects of BDNF protein, the effects on metabolism should also be investigated. In this study, glucose, lipid and protein parameters were aimed to compare among BALB/C, BDNF homozygous (+/+) and BDNF heterozygous transgenic (+/-) mice. Ten BALB/C mice and 9 wild type (WT) BDNF homozygous normal (+/+) mice and 4 WT BDNF heterozygous transgenic (+/-) mice were used in this experiment. BDNF heterozygous transgenic (+/-) and BDNF homozygous mice were differentiated by RT-PCR. When the data obtained in the groups were compared, it was determined that glucose and lipid levels in WT groups were significantly low and total protein level was high in comparison to BALB/C group. It was detected that no significant difference among these parameters was found between BDNF homozygous (+/+) and BDNF heterozygous transgenic (+/-) mice. It was understood that BDNF protein deficiency does not significantly affect glucose, lipid and protein levels in healthy normal mice. Further studies are needed to evaluate the effects of BDNF protein on metabolism. Investigations about the role of BDNF during metabolic diseases may provide to comprehend the metabolic effects more

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