Buket BAKIR, Ebru KARADAĞ SARI, Seyit Ali BİNGÖL

Muhabbet Kuşu (Melopsittacus undulatus) ve Hint Bülbülü (Taeniopygia guttata) Yumurta Kabuğunun Elemental ve Ultrastrüktürel Yapısının Taramalı Elektron Mikroskop ile Karşılaştırılması

Bu çalışmada, muhabbet kuşu (Melopsittacus undulatus) yumurta kabukları ile hint bülbülü (Taeniopygia guttata) yumurta kabuklarının element düzeyleri ve yapısal özellikleri taramalı elektron mikroskop kullanılarak incelenmesi amaçlandı. Çalışmada 10 adet muhabbet kuşu ve hint bülbülü yumurtaları kullanıldı. Yumurta kabukları bir gece distile suda bekletildikten sonra kalan zarlar uzaklaştırıldı ve oda ısısında 24 saat kurumaya bırakıldı. Herbir yumurta kabuğunun ekvator ve kutup bölgelerinden yaklaşık 0.5 cm2 ebatlarında örnekler alındı. Yumurta kabuğu örnekleri iç, dış ve dikey olacak şekilde stublara yapıştırıldı. Örnekler taramalı elektron mikroskobunda element oranı ve yapı bakımından incelendi. Yumurta kabuklarının dış tabakalarında kalsiyum (Ca), oksijen (O), karbon (C) iç tabakalarında ise Ca, O2, C, azot (N) ve kükürt (S) elementlerinin yer aldığı tespit edildi. Muhabbet kuşunda mamillary ve palisade tabakalarının hint bülbülü mamillary ve palisade tabakalarına göre daha kalın olduğu görüldü. Sonuçta, muhabbet kuşu ve hint bülbülü yumurta kabuklarının benzer özellikler gösterdiği saptandı.

Comparison of Elemental and Ultrastructure of Eggshells of Budgerigar (Melopsittacus undulatus) and Zebra Finch (Taeniopygia guttata) with Scanning Electron Microscope

The purpose of this study was to investigate elemental and ultrastructural analysis of eggshells of Budgerigar (Melopsittacus undulatus) and Zebra finch (Taeniopygia guttata) using scanning electron microscope. Ten Budgerigar's eggshells and Zebra finch's eggshells were used in this study. The remaining of eggshell membranes were removed after the eggshells were incubated with distilled water overnight and were allowed to dry at room temperature over 24 h. About 0.5 cm2 section was cut from part of equatorial and pole of each eggshell. The Shell was fixed inner, outer and vertical posture to stubs. The samples were examined ratio of element and ultrastructure under scanning electronmicroscope. It was observed calcium (Ca), oxygen (O2), carbon (C) in outer layer and Ca, O2, C, nitrogen (N) and sulfur (S) in inner layer of eggshell membrane. It was determined mamillary and palisade layer of budgerigar's eggshell were thicker than mamillary and palisade layer of zebra finch's eggshell. In conclusion, it was determined eggshells of budgerigar and eggshells of Zebra finch are similar properties.

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