Light and Scanning Electron Microscopic Investigation of Postnatal Development of Vallate Papillae in the White Laboratory Mice

Çalışmada; laboratuar farelerinde papilla vallata'da meydana gelen ışık ve taramalı elektron mikroskopik postnatal değişimlerin ortaya konulması amaçlanmıştır. Bu amaç için 1, 15, 30, 60, 90, 120, 150 ve 180 günlük 8 grup oluşturuldu ve her grupta 8'er fare kullanıldı. Ayrıca ilk olgun tat tomurcuğunun oluşum zamanının tespiti için de 0-10. günler arasında her gün iki fare kullanılmak üzere, toplam 84 fare kullanıldı. Dilin kök bölgesi dorsal yüzünde median hat üzerinde yerleşmiş tek olarak bulunan papilla vallata'da yapılan mikrometrik ölçümlerde ilk 15 günlük periyotta çok hızlı bir gelişimin olduğu dikkat çekicidir. Papilla genişliği ve uzunluğu ile hendek derinliği ve genişliğindeki artış bu periyottan sonra yavaş bir artış göstererek 120. güne kadar devam etmektedir. Tat porusu şekillenmiş ilk olgun tat tomurcuğu 4. günde görülmüştür. Tat tomurcuğu uzunluk ve genişliğindeki artış 90. güne, tat tomurcuğu sayısındaki artış ise 30. güne kadar devam etmektedir. Hendek duvar yüzey alanı da yine ilk 15 günlük periyotta % 136'lık bir artış göstermekte, bu periyottan sonra ise 90. güne kadar yaşın ilerlemesine paralel bir artış göstermektedir. Papilla vallatanın taramalı elektron mikroskobik yapısında yüzey epitel hücrelerinin sınırları belirgin ve hücre yüzeylerinde micropit ve microridge mevcuttu.

Beyaz Laboratuvar Farelerinde Papilla Vallatanın Postnatal Gelişimi Üzerine Işık ve Taramalı Elektron Mikroskobik İncelemeler

The aim of this study was to investigate postnatal changes that might occur on vallate papilla of laboratory mice by using light and scanning electron microscopy (SEM). Eight groups were formed for 1, 15, 30, 60, 90, 120, 150 and 180 days old mice and 8 mice were used in each group. Micrometric measurements of vallate papilla which was located median line on dorsal surface of radix area of tongue showed that development of this papilla was very fast for first 15 days period. After then, increases in length and width of papilla and depth and width of trench were slowed down but development continued to 120th day. First mature taste buds which had taste pores were seen on 4th the day of the age. Increase of length and width of taste buds continued by 90th day, increase of number of taste buds continued by 30th days. Trench wall surface area increased 136% in first 15 days period, after this period by 90th day it develop in parallel to the age. In the structure of scanning electron microscopy of vallate papilla, epithelial cell-margin thickness was evident and had micropits and microridges on the surface cells. As a result, it is detected that vallate papillae developed by 120th day and taste buds developed and became functional in the first 4 days.

PDF

___

1. Iwasaki S., Miyata K., Kobayashi K., 1987. The surface structure of the dorsal epithelium of tongue in the mouse. Acta Anatomica Nipponica, 62, 69-76.
2. Kinnamon CJ., Taylor BJ., Delay RJ., Roper SD., 1985. Ultrastructure of mouse vallate taste buds. I. Taste cells and their associated synapses. Journal of Comparative Neurology, 235, 48-60.
3. Utiyama C., Watanabe I., Konig B., Koga LY., Semprini M., Tedesco RC., 1995. Scanning electron microscopic study of the dorsal surface of the tongue of Calomys callosus mouse. Annals of Anatomy, 177, 569-572.
4. Watanabe I., Utiyama C., Koga LY., Motoyama AA., Kobayashi K., Lopes RA., König B., 1997. Scanning electron microscopy study of the interface epithelium-connective tissue surface of the lingual mucosa in Calomys callosus. Annals of Anatomy, 179, 45-48.
5. Miller RL., Chaudhry AP., 1976. Comparative ultrastructural of vallata, foliate and fungiform taste buds of golden Syrian hamster. Acta Anatomica, 95, 75-92.
6. Miller IJ., Smith DV., 1984. Quantitative taste bud distribution in the hamster. Physiology & Behavior, 32, 275-285.
7. Toyoshima K., Shimamura A., 1979. The occurrence of ciliated and mucous cells in the peripapillary trench of the rat tongue. Anatomical Record, 195, 301-310.
8. Yılmaz S., Dinç G., Aydın A., Girgin A., 1995. Ratlarda papilla vallata ve tat tomurcuklarının postnatal mikrometrik değişimleri ve gelişimi. Turkish Journal of Veterinary and Animal Science, 19, 193-198.
9. Kubota K., Fukuda N., Asakura S., 1966. Comparative anatomical and neurohistological observations on the tongue of the porcupine (Hystrix cristata). Anatomical Record, 155, 261- 268.
10. Kubota K., Togawa S., 1966. Comparative anatomical and neurohistological observations on the tongue of japanese dormouse (Glirus Japonicus). Anatomical Record, 154, 545-552.
11. Emura S., Tamada A., Hayakawa D., Chen H., Jamali M., Taguchi H., Shoumura S., 1999. SEM Study on the dorsal lingual surface of the Flying Squirrel (Petaurista leucogenys). Annals of Anatomy, 181, 495-498.
12. Kobayashi K., 1990. Three-dimensional architecture of connective tissue core of the lingual papillae in the guinea pig. Anatomy and Embryology, 182, 205-213.
13. Miller IJ., Smith DV., 1988. Proliferation of taste buds in the foliate and vallate papillae of postnatal hamsters. Growth, Development and Aging, 52, 123-131.
14. Zalevski AA., 1970. Regeneration of taste budsbin kerebin kerebin kere in the lingual epithelium after excision of the vallata papilla. Experimental Neurology, 26, 621-629.
15. Ahpin P., Ellis S., Arnott C., Kaufman MH., 1989. Prenatal development and innervation of the circumvallate papilla in the mouse. Journal of Anatomy, 162, 33-42.
16. Paulson RB., Hayes TG., Sucheston ME., 1985. Scanning electron microscope study of tonguebin kere development in the CD-1 mouse fetus. Journal Cranofacial Genetics and Developmental Biology, 5, 59-73.
17. Kaufman MH., 1992. The Atlas of Mouse Development. 421-423, Academic Press, San Diego.
18. Iwasaki S., Yoshizawa H., Kawahara I., 1996. Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the mouse. Acta Anatomica, 157, 41-52.
19. Kinnamon CJ., Sherman TA., Roper SD., 1988. Ultrastructure of mouse vallate taste buds: III. Patterns of synaptic connectivity. Journal of Comparative Neurology, 270, 1-10.
20. Krause WJ., Cutts JH., 1982. Morphological observations on the papillae of the opossum tongue. Acta Anatomica, 113, 159-168.
21. State FA., Bowden REM., 1974. Innervation and cholinesterase activity of the developing taste buds in the circumvallate papilla of the mouse. Journal of Anatomy, 118, 211-221.
22. Hosley MA., Oakley B., 1987. Postnatal development of the vallata papilla and taste buds in rats. Anatomical Record, 218, 216-222.
23. Misretta CM., Baum BJ., 1984. Quantitative study of taste buds in fungiform and circumvallate papillae of young and aged rats. Journal of Anatomy, 138, 323-332.
24. Iwasaki S., Yoshizawa H., Kawahara I., 1997. Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the rat. Anatomical Record, 247, 528-541.
25. Smith DV., Miller IJ., 1987. Taste bud development in hamster vallate and foliate papillae. Annals of the New York Academy of Sciences, 510, 632-634.
26. Harada S., Yamaguchi K., Kanemaru N., Kasahara Y., 2000. Maturation of taste buds on the soft palate of the postnatal rat. Physiology & Behavior, 68, 333-339.
27. Cano J., Roza C., Rodriguez-echandia EL., 1978. Effects of selective removal of the salivary glands on taste bud cells in the vallate papilla of the rat. Experientia, 34, 1290-1291.
28. Dmitrieva NA., 1986. Histogenesis of the taste buds of the vallate papilla in the rat in the postnatal stages of development. Tsitologiia, 28, 745-748.
29. Misretta CM., Goosens KIA., Farinas I., Reichardt LF., 1999. Alterions in size, number, and morphology of gustatory papillae and taste buds in BDNF null mutant mice demonstrata neurol dependence of developing taste organs. Journal of Comparative Neurology, 409, 13-24.
30. Iwasaki S., Miyata K., Kobayashi K., 1988. Scanning electron microscopic study of the dorsal lingual surface of the squirrel monkey. Acta Anatomica, 132, 225-229