Turgay DEPREM, Seyit Ali BİNGÖL, Serap TAŞCI KORAL, Şahin ASLAN, Nurhayat GÜLMEZ YECAN

Kaz (Anser anser) Dalak Dokusunda Histolojik ve Histometrik İnceleme

: Bu çalışmada Kars ta yetiştirilen kazların ( Anser anser) dalağının histolojik ve histometrik olarak incelenmesiamaçlandı. Materyal olarak 10- 12 aylık 6 adet dişi kaz kullanıldı. Dalaktan alınan örnekler rutin doku takibinden geçirilerekCrossman ın üçlü boyaması, Hematoksilen Eosin boyama ve Toluidin mavisi ile boyandı. Kaz dalağının etrafının bağdokudanoluşmuş bir kapsül ile çevrelendiği, trabeküllerin organın içine doğru giderek inceldiği gözlendi. Dalak dokusunda beyaz vekırmızı pulpa alanları birbirinden ayırt edilirken, beyaz pulpada lenf foliküllerinin belirgin olduğu görüldü. Yapılanhistometrik ölçümlerde, kaz dalağını çevreleyen kapsül kalınlığı ortalaması 22 µm olarak ölçüldü. Lenf folikülü sayısı 1.07mm2 de ortalama 2.4 olarak belirlendi. Mast hücresinin ortalama çapı 6 µm olarak ölçüldü. Mast hücre sayısı 1.07 mm2 deortalama 1.4 olarak belirlendi. Kaz dalağının histolojik yapısı birçok yönden diğer kanatlılarla benzerlik göstermesine karşıntrabeküllerin daha belirgin olması nedeniyle memeli dalağına daha benzer yapıda olduğu görüldü.

Histologic and Histometric Examination of Spleen in Geese (Anser anser)

The aim of this study was to examine the histometrical and histological structures of goose (Anser anser) spleen. Six healthy female geese were used as material. Tissue samples taken from the spleen were processed routinely, and were then stained with H&E, Crossmans Triple stain and Toluidine blue stain. The spleen surrounded by capsules composed ofconnective tissue and parts of the capsules were observed increasingly thinning into the spleen as trabeculae. The red pulparea was distinguishable from the white pulp inside the organ; further, the lymph follicles appeared clearly within the whitepulp. Histometric measurements revealed that the thickness of the capsules surrounding the organ ranged from 18 to 28µm. The average thickness of the capsules was measured as 22 µm. The average number of lymph follicles was found to be2.4 in 1.07 mm2 . The average width and length of the lymph follicles were measured as 113 µm and 144 µm, respectively.The average diameter of the mast cells was found to be 6 µm. The average number of mast cells was found to be 1.4 in 1.07mm2 . Although the histological structures of the geese spleens seemed very similar to those of other animals in severalrespects, but some specific properties of geese spleens being more similar to that of mammalians were also observed.

PDF

___

Alabay B., 2008. Lymphoid system. In “Veteriner Özel Histoloji”, Ed., A Ozer, 49-66, Nobel Press, Ankara.
Asti RN., Tanyolac A., Kurtdede N., Ozen A., 1997. Distribution of T and B lymphocytes in Angora Goat tissues. Turkish Journal of Veterinary & Animal Sciences, 21, 99-105.
Balcan E., Arslan O., Gumus A., Sahin M., 2009. The effect of tunicamycin on embryonic and newborn murine spleen tissues. Journal of the Faculty of Veterinary Medicine, Kafkas University, 15, 651-660.
Biljana M., Ana R., Ruzica A., Ljiljana D., Mileva M., 2008. Changes in lymphatic organs of layer chickens following vaccination against marek’s disease: histological and stereological analysis. Acta Veterinaria (Beograd), 58, 3-16.
Bradley OC., 1915. The structure of the fowl. 79-88. A.& C. Black, LTD. London.
Caughey G.H., 2011. Mast cell proteases as protective and inflammatory mediators. Advances in Experimental Medicine and Biology, 716, 212-234.
Causey Whittow G., 2000. Strukie’s Avian Physiology, 5th ed., 659-661. Academic Press. London.
Fitzgerald TC., 1969. The Coturnix Quail. Anatomy and Histology. 237-238. The Iowa State University Press, Ames, Iowa.
Galli SJ., Dvorak AM., Dvorak HF., 1984. Basophils and mast cells: morphologic insights into their biology, secretory patterns, and function. Progress in Allergy, 34, 1-141.
Hodges RD., 1974. The Histology of the Fowl. 221- 225. Academic Press. London.
Jamur MC., Grodzki ACG., Berenstein EH., Hamawy MM., Siraganian RP., Oliver C., 2005. Identification and characterization of undifferentiated mast cells in mouse bone morrow. Blood, 105, 4282-4289.
Jeurissen SHM., Janse EM., Ekino S., Nieuwenhuis P., Koch G., De Boer GF., 1988. Monoclonal antibodies as probes for defining cellular subsets in the bone marrow, thymus, bursa of fabricius, and spleen of the chicken. Veterinary Immunology and Immunopathology, 19, 225- 238.
Karaca T., Yoruk M., Uslu S., 2006. Age-Related distribution and heterogeneity in the number of mast cells lymphoid of the organs in turkey. The Journal of the University of Yuzuncu Yil, Faculty of Veterinary Medicine, 17, 5-8.
Kozlu T., Karadag Sari E., Akaydin Bozkurt Y., Altunay H., 2011. A comparative study on the histological structure of the spleen in the ostrich (Struthio camelus), the kestrel (Falco tinnuculus), and the osprey (Pandion haliaetus). Acta Biologica Hungarica, 62, 113- 121.
Kroese FGM., Wubbena KC., Kuijpers KC., Nieuwenhuis P., 1987. The ontogeny of germinal centre forming capacity of neonatal rat spleen. Immunology, 60, 597-602.
Liman N., Bayram GK., 2011. Structure of the quail (coturnix coturnix japonica) spleen during preand post-hatching periods. Revue de Medecine Veterinaire, 162, 25-33.
Luna LG., 1968. Manual of Histologic Staining Methods of Armed Forces Institute of Pathology, 3rd ed., 32-46. Mc Graw-Hill Book Comp., London.
Payne LN., 1971. The Lymphoid System. In “Physiology and Biochemistry of the Domestic Fowl”, Ed., Bell DJJ., Freeman BM., 985-1037. Academic Press. London.
Ross MH., Pawlina W., 2011. Histology: A Text and Atlas, 6 th ed., 471-474. Lippincott Williams and Wilkins. Baltimore, Philadelphia.
Starck JM., Riclefs RE., 1998. Avian growth and development: Evolution within the altricialprecocial. 205-208. Oxford University Press. New York.
Straus AH., Nader HB., Dietrich CP., 1982. Absence of heparin or heparin-like compounds in mastcell-free tissues and animals. Biochimica Biophysica Acta, 717, 478-485.
Thangapandiyan M., Balachandran C., 2010. Cytological evaluation of canine lymphadenopathies- a review of 109 cases. Veterinarski Arhiv, 80, 499-508.
Thorbecke GJ., Gordon HA., Wostman B., Wagner M., Reyniers JA., 1957. Lymphoid tissue and serum gamma globulin in young germfree chickens. The Journal of İnfectious Diseases, 101, 237-251.
Tischendorf F., 1985. On the evolution of the spleen. Experientia, 41, 145-152.
Uslu S., Yoruk M., 2013. Morphological and histometric studies on mast cell distribution and heterogeneity, present in the lower respiratory tract and in the lung of local duck (Anas platyrhnchase) and goose (Anser anser). Journal of the Faculty of Veterinary Medicine, Kafkas University, 19, 475-482.