Leyla MİS, Bahat COMBA, Abuzer TAŞ, Arzu COMBA, Ali ÇINAR

Deneysel Olarak Diabet Oluşturulmuş Ratlarda Yara İyileşmesinde Sildenafil Sitratın Bazı Hematolojik Parametrelere ve Mineral Maddelere Etkisi

Bu çalışmada sildenafil sitratın yara iyileşmesinin erken dönemlerinde bazı hematolojik parametreler ve mineralmaddeler üzerine olan etkisi araştırıldı. Bu amaçla her grupta 10ar adet rat olacak şekilde üç grup (1. Grup: Kontrol, 2.Grup: Diabet ve 3. Grup: Diabet + Sildenafil Sitrat) oluşturuldu. Birinci ve ikinci gruba normal yara bakımı, üçüncü gruba isenormal yara bakımının yanında 0.7 mg/kg dozunda 3 gün süreyle günde bir kez olmak üzere intraperitonal (i.p.) sildenafilsitrat verildi. Uygulamalara başlamadan önce 1. 2. ve 3. günlerde kan alındı. Tam kanda eritrosit (RBC), hemoglobin (HG),hematokrit (HCT), değerlerine klasik yöntemlerle; serumda demir(Fe), mangan (Mn), bakır (Cu), çinko (Zn), kurşun (Pb),potasyum (K), kalsiyum (Ca) ve magnezyum (Mg) değerlerine ise spektrofotometrik metodla bakıldı. Sildenafil sitrat, diabetliyaralarda iyileşme sürecine olumlu etki eden mineralllerden Zn, Cu ve Mg değerlerini arttırdı. İlk dönemlerde iyileşmesürecine olumsuz etki eden Fe minerali değerlerini düşürdü. Sonuç olarak, bu şekilde yara iyileşmesinin ilk dönemi olanyangısel dönemi kısaltarak, iyileşme sürecine olumlu etki yaptığı kanaatine varıldı.

The Effects of Sildenafil Citrate on Some Haematological Parameters and Mineral Matters in Wound Healing of Rats Created Experimental Diabetes

: In this study, the effect of sildenafil citrate on some haematological parameters and mineral levels in the earlystages of wound healing was investigated. Rats were randomly allocated into three treatment groups consisted of 10 rats ineach. Groups were designed as control (group I), diabetic (group II) and diabetic plus sildenafil citrate (group III),respectively. Normal wound care was performed for control and group II. Sildenafil citrate was also administered withnormal wound care for group III at 0.7 mg/kg dosage for 3 days via intraperitoneally (i.p.) once a day. Blood samples werecollected before and at 1., 2., and., 3. days of after administration. Full blood erythrocyte (RBC), haemoglobin (HG),haematocrit (HCT) values were determined using standard methods; spectrophotometric methods were used fordetermination of the serum iron (Fe), manganese (Mn), copper (Cu), zinc (Zn), lead (Pb), potassium (K), calcium (Ca), andmagnesium (Mg) levels. Sildenafil citrate increased the serum mineral levels (i.e. Zn, Cu and Mg), as having favourableimpact on diabetic wound healing process. Likewise, it decreased the level of iron mineral, as having an adverse effect onthe healing process. In conclusion, it was concluded that sildenafil citrate had a favourable effect on the healing process byshortening the inflammatory period, which is the first period of wound healing process.

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