Genetic diversity in prunus spinosal. and challenges in the use of autochthonous sources

Almanyanın farklı bölgelerindeki çakal eriği ( Prunus spinosa L.) doğal türünün genetik çeşitliliği, yüksekyapışma sıcaklığında rastgele çoğaltılmış polimorfik DNA (HAT - RAPD) protokolü kullanılarak analizedilmiştir. Bulgular daha önceki çalışmalarda kaydedilen benzer bölgelerden toplanmış tohumstoklarından elde e dilen örneklerin bulgularıyla karşılaştırılmıştır. Genellikle doğal türlerdeki (suş) genetikçeşitlilik önceki çalışmalarda ölçülen değerden (Ho: 0,118 - 0,113) düşük olduğu (Ho; 0,099 -0,116)belirlenmiştir. Dahası genetik farklılaşma doğal türlerde orta düz eyde (Fst; 0,138 - 0,184; populasyonlararası varyasyon oranı % 22,527) ama tohum stoklarından elde edilen örneklerin değerlerinden (Fst; 0,086 -0,104; populasyonlar arası varyasyon oranı % 7,782) daha yüksek olduğu belirlenmiştir. Bulgular Almanyakoruma kanunları ve uygulamaları bakımından tartışılmıştır.

Prunus spinosa l. nin genetik çeşitliliği ve yerli popülasyon kaynaklarının kullanımındaki zorluklar

Genetic diversity in natural stands of autochthonous blackthorn ( Prunus spinosa L.) of different Germanprovenances has been analyzed using a highly reproducible high- annealing- temperature random ampliﬁ edpolymorphic DNA (HAT -RAPD) protocol. The findings were compared to those from seedstocks of thesame provenances, reported earlier. Generally, genetic dive rsity in the natural stands was even lower (Ho0.0990.116) compared to the corresponding seedstocks (Ho 0.1180.133). Furthermore, geneticdifferentiation was found to be moderate between natural residential sources (pairwise Fst 0.1380.184,22.527% vari ation among populations), but higher than between the seedstocks (pairwise Fst 0.086 -0.104,7.782% variation among populations). The findings are discussed in respect to German conservation lawand its practical implementation.

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