Zafer DOĞU, Faruk ARAL, Erdinç ŞAHİNÖZ, Füsun TEMAMOĞULLARI

Gökkuşağı Alabalık (Oncorhynchus mykiss W., 1792)Spermasında Motilite, Canlılık ve Fertilizasyon Üzerine Bir Sulandırıcı Olarak Üzüm Pekmezinin Etkisi

Bu çalışmada, gökkuşağı alabalıklarının (Oncorhynchus mykiss) dondurulan spermasına % 5, 7 ve 10 oranında katılan pekmezin sperma motilitesi, hareketliliği ve döllenme oranı üzerine etkisi incelendi. Altı adet erkek balık sperması % 7 dimetil sülfoksit ile birlikte % 5, 7 ve 10 oranında pekmez içeren sulandırıcı ile sulandırıldı. Spermalar 0.25 ml payetlerde, sıvı azot buharında dondurularak 2 hafta boyunca sıvı azot içerisinde muhafaza edildi. Spermatozoa motilitesi üzerine pekmez etkisi açısından, %10 oranında üzüm pekmezinin (P<0.001) daha faydalı olduğu ve (P>0.05) döllenme oranı üzerine pekmezin tüm oranlarında önemli bir fark bulunamadığı saptandı. Ancak, % 10 oranında kullanılan pekmez ile dondurulan spermalardan elde edilen döllenme oranı glikoz sulandırıcısı için elde edilen en yüksek değerden daha iyi oldu (96.4%). Çalışma sonuçlarına göre, % 10 oranında pekmezin gökkuşağı alabalıklarının spermalarının dondurulmasında kullanılabileceği kanaatine varıldı.

Anahtar Kelimeler:

Yetiştiricilik, dondurma, kriyoprezervasyon, sperm

The Effect of Grape Molasses as an Extender on Motility, Viability and Fertility in Rainbow Trout (Oncorhynchus mykiss W., 1792) Sperm

In this study, the effects of three different dosages of grape molasses (5, 7, 10 %) as a diluent were investigated on post‐thaw motility, sperm viability and fertilization rate of frozen spermatozoa from the rainbow trout (Oncorhynchus mykiss). Semen from six male fish sperm was diluted with in extenders containing 5, 7, and 10% grape molasses with dimethyl sulfoxide 7%. The semen was frozen in 0.25 ml straws in liquid nitrogen vapor and stored in liquid nitrogen for 2 weeks. From the point of grape molasses effect over sperm motility, grape molasses level of 10 % was found more beneficial (P<0.001), and there was no significant difference between various level of grape molasses on fertilization rate (P>0.05). But, the 10% grape molasses solution was better (96.4%) than the glucose solution which has the highest fertilization rate (P<0.05). As a result of the study, the level of 10% grape molasses is given that might be used to freezing rainbow trout sperm.

Keywords:

Aquaculture, freezing, cryopreservation, milt,

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