Paul Onu Ajah, Efio-Okon Bassey Ita, Nneka Love Allison

Characterization of Nematode Infestation on Parachanna obscura (Gunther, 1861) (Channidae) and Infection in the Blood

Parachanna obscura commands high food value and quite critical in the control of tilapia population in ponds. The health status of fish is assured upon proper diagnosisof the blood parameters. Research on fifty P. obscura from Great Kwa River, Nigeria investigated, showed 24 (48%) were infested with nematode and infected the bloodwhile 26 (52%) were not. The mean blood Pack cell volume (PCV), Lymphocyte, Monocyte, Leucocyte, Neutrophils, and Eosinophils for infected(non-infested) P.obscura respectively were 20.8% (21.6%); 44.4% (39.3%); 1.9% (1.92%); 4.18x103mm3 (4.48x103mm-3 ), 51.7% (57.5%). and 1.58% (1.2%). No positive correlation existedbetween infested and non-infested. The lower PCV, Leucocyte, and Neutrophils are indications of lower immunity. Prevalence rate increased with size from 26% (22-26.9cm), 56.5% (27-31.9cm) to 66.7% at class range 32-36.9cm, while Incidence of infestation decreased with increase in length, 2.75, 2.25 and 2.0 respectively for 22-26.9cm, 27-31.9cm and 32-36.9cm. The class 12-16.9cm did not follow any pattern. The parasitic burden occurred at the weaker/more vulnerable part-anal pore (61.7%), followed by fish intestine (29.8%), cardiac (4.3%) and caecum (4.3%). The meanphysicochemical parameters of the study area across the three tidal intervals ranged from 5.11±0.04-6.72±0.00 for pH, conductivity 18.70±0.20µS-37.95±1.05 µS, dissolved oxygen 2.60±0.00 mg/l- 4.00±0.60 mg/l, temperature 25.50±0.50°C-27.00±0.00°C,salinity 0.01±0.00mg/l-0.03±0.01mg/l and total dissolved solid (TDS) 23.19±0.25mg/l47.06±1.30mg/l.

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Adebayo, O.T., Fagbenro, O.A., Ajayi, C.B. & Popoola, O.M. (2007). Normal haematological profile of Parachanna obscura as a diagnostic tool in aquaculture. International Journal of Zoological Research, 3, 193-199.
Adeyemo, O.K., Okwilagwe, O.O. & Ajani, F. (2009). Comparative assessment of sodium edta and heparin as anticoagulants for the evaluation of haematological parameters in cultured and feral African catfish (Clarias gariepinus). Brazilian Journal of Aquatic Science and Technology, 13(1), 19-24.
Ahmed, I & Sheikh, Z. A. (2020). Comparative study of hematological parameters of snow trout Schizopyge plagiostomus and Schizopyge niger inhabiting two different habitats, The European Zoological Journal, 87,1, 12-19. http://dx.doi.org/10.1080/24750263.2019.1705647
Ajah P.O., & Ukutt, E.R. (2018a). The in vitro effect of water soluble fraction of crude oil on the biochemical, hematological and enzymological parameters of clariid catfish juveniles and sub adults. International Journal of Scientific and Engineering Research (IJSER), 9(12),1364- 1385.
Ajah P.O., & Ukutt, E.R. (2018b). The effect of water-soluble fraction of crude oil on the biochemical, hematological and enzymological changes in fed and starved clariid catfish juveniles. International Journal of Scientific and Engineering Research (IJSER), 9(4), 1386-1406.
Akther, S., Hakkani, E. Aman, A & Mohanta, M. (2018). Prevalence of parasitic infestations in the freshwater fish, Chann punctatus (Bloch) from Rajshahi Metropolitan, Bamgladesh. International Journal of Current Microbiology and Applied Sciences, 7(04), 3431- 3441. DOI: 10.20546/ijcmas/2018.704.388.
Ama-Abasi, D., & Anthony, O. (2010). Proximate analysis of snakeshead, Parachanna obscura, (Gunter 1861) in the Cross River, Nigeria. Global Journal of Agricultural Sciences, 9(2), 7-13.
Anderson, D., & Klontz, G.W. (1965). Basic Haemotology for the fish culturist Ann. Northwest. Fish cult., 16, 38-41.
Bassey, A.U. & P.O. Ajah (2010). Effect of three feeding regimes on growth, condition factor and food conversion rate of pond cultured Parachanna obscura (Gunther, 1861) (Channidae) in Calabar, Nigeria. Turkish Journal of Fisheries and Aquatic Sciences, 10, 195-202.
Boon, J.H., Zuxu, Y. &. Boom, G.H.R (1990). Effects on Anguillicola crassus and Trypanosoma granulosum infections on peripheral blood cells of European Eel (Anguilla anguilla L). Bull. Eur. Ass. Fish Pathol., 10 (5), 143-145.
Clauss, T.M., Alistair, D.M. & Arnold, J.E. (2008). Hematological disorders of fish. Veterinary Clinics of North America: Exotic Animal Practice, 11(3), 445-462.
Collazos, M.E., Ortega, E., Barriga, C. & Rodriguez, A.B. (1998). Seasonal variation in hematological parameters male and female Tinca tinca, Molecular and Cellular Biochemistry, 183(1-2), 165-168. http://dx.doi.org/10.1023/A:1006878922332
Conver, D F. Spadling, M.G. & Forester, D.J. (2003). Epizootiology of Eustrongylides ignotus in Florida: Transmission and development of larvae in intermediate hosts. Journal of Parasitology, 89(2), 290 – 298
Dacie, J.V. & Lewis, S.M (1984). Practical Haematological 8th edition Edinburgh, Livingstone. 50 – 57.
Gado, M.S.M., Mahfouz, N.B., Moustafa, E.M.M. & Lolo, E.E.E. (2017). Prevalence of some ectoparasitic diseases in African catfish (Clarias gariepinus) at Kafr El-Sheikh governorate. International Journal of Fsheries and Aquatic Studies, 5(3), 576-583.
Garcia, M. P., Echevarrin, G., Martinez, F. J. & Zamora, Z., (1992). Influence of blood sample collections on the haematocrit value of two teleosts, rainbow trout (Oncohrhynchus mykiss) and European sea bass (Dicentrachus labrax L.). Comp. Biochem.Physiol., 101, 733–736.
George, A.D.I., Akinrotimi, O.A. & Nwokoma, U.K. (2017). Haematological Changes in African Catfish (Clarias gariepinus) Exposed to Mixture of Atrazine and Metolachlor in the Laboratory, Journal of Fisheries Science.com, 11(3), 048-054.
Gupta, S., & Banerjee, S. (2011). Assessment of some haematological parameters of an airbreathing fish Heterpneustes fossilis-After short term exposure to sublethal concentrations of Mercuric Chloride. Fishing Chimes, 31(8), 1-4.
Guseva, L.N. (1990). Food Diets and feeding ratio in snakehead Channa argus in the lower A muder’s ya R. J. Ofo. Lethy Oil, 30, 439-466.
Ibiwoye, T.I.I., Owolabi, O.D., Ajala, A.A., Oketoki, T.O., Adio, S.M., Adedapo, A., Ayeku, P.O. & Agbontale, J.J. (2006).
Helminth parasites in freshwater fish species from Jebba Lake and Bida floodplain areas of River Niger, Nigeria. Proceedings of Conference of the Fisheries Society of Nigeria, 16(20), 13-20.
Ighwela, K. A.; Ahmad, A. B. & Abol-Munafi, A. B., (2012). Haematological changes in Nile tilapia (Oreochromis niloticus) fed with varying dietary maltose levels. World J. Fish Mar. Sci.4. 376–381.
Imanpoor, M.R., Bagheri, T., & Hedayati, S.A.A. (2010). The anaesthetic effects of clove essence in persian sturgeon, Acipenser persicus. World Journal of Fish and Marine Sciences, 2(1), 29-36.
Jerônimo, G.T., Pádua, S.B., Bampi, D., Gonçales, E.L.T., Garcia, P., Ishikawa, M.M., & Martins, M.L. (2014). Haematological and histopathological analysis in South American fish Piaractus mesopotamicus parasitized by monogenean (Dactylogyridae). Brazilian Journal of Biology, 74(4), 1000-1006. http://dx.doi.org/10.1590/1519-6984.09513.
Khan, R.K, Khatoon, N., Muhammad, F., & Shafi, M. (2019). Seasonal variation of parasitic infections in fish Johniuss dussumieri (Perciformes: Sciaenidae). International Journal of Aquatic Science, 10(2), 94-97. ISSN: 2008-8019.
Kori-Siakpere, O. & Egbor, V.E. (1997). Haematological characteristics African mudfish: Clarius buthupogon (Clariidae), Bul., 21, 177 –185.
Kori-Siakpere, O., Ake, J.E.O. & Idoge, E. (2005). Haematlogical characteristics of the African snakehead, Parachanna obscura, African Journal of Biotechnology, 4(6), 527-530.
Kumari, A., & Perven, S. (2017). Comparative study of helminth parasites in freshwater fish, Channa punctatus of Chapra, Bihar. International Journal of Scence and Research, 6(8), 1369.
Lavanya, S.; Ramesh, M.; Kavitha, C. & Malarvizhi, A., (2011). Hematological, biochemical and ion regulatory responses of Indian major carp Catla catla during chronic sub lethal exposure to inorganic arsenic. Chemosphere, 7, 977–985
Lewis, S.M. & Ward, P.G. (1975). An evaluation of Haematological Laboratory Practice. 24:13
Labello, S.L., Saunder, D.K. & Crawford, T.G. (2001). Observation on the blood viscosity in striped bass, Morore saxatilis (Coal baum) associated with fish hatchery condition Transaction of the Kansas Academy of Science, 104 (314), 183 – 194
Margolis, L., Esch, G.W., Holmes, J.C., Kuris, A.M., & Schad, G.A. (1982). The use of ecological terms in parasitology (Report on an ad.hoc committee of the American Society of Parasitologist). Journal of Parasitology, 68, 131-133.
Mazumder, S.K., Fivelstad, S., Ghaffar, M. Abd., & Das, S.K.(2019). Haematological and biochemical responses of juvenile Malabar blood snapper (Lutjanus molabaricus Bloch & Schneider, 1801) exposed to different rearing temperatures and diets. Sains Malaysiana, 48(9), 1790- 1810. http://dx.doi.org/10.17576/jsm-2019-4809-01
Moravec, F. (2000). Nematodes of the freshwater fishes of the Neotropical region. Folia Parasitologia, 47, 60-66. Nazeef, S, & Abubakar, U.M. (2013). Diversity and condition factor of fish species of DadinKowa Dam, Gombe State, Nigeria. Greener Journal of BiologicalSciences, 3(10),350-356.
Obiekezie, A.I. & Ajah, P. O. (1994). Chemotherapy of Macrogyrodactylosis in the culture of African clariid catfish: Clarias gariepinus and Heterobranchus longifilis. J. Aqua. in the Tropics, 9, 187-192.
Oladipo, S.O., Sunday, O.J., & Ogunbiyi, D.C. (2019). Occurrence and prevalence of parasites associated with Gnathonemus senegalensis in Apodu Reservoir, Malete, Nigeria. Sri Lankan Journal of Biology, 4(1), 14-23.
Pradhan, S. C.; Patra, A. K.; Mohanty, K. C.; & Pal, A., (2012). Hematological and plasma biochemistry in Cirrhinus mrigala (Hamilton 1822). Comp. Clin. Pathol., http://dx.doi.org/10.1007/s00580-012-1642-z .
Pradhan, S.C., Patra, A.K. & Pal A. (2014). Hematological and plasma chemistry of Indian major carp, labeo rohita (Hamilton, 1822). Journal of Applied Ichthyology, 30(1), 48-54. http://dx.doi.org/10.111/JAI.12297
Satheeshkumar, P., Ananthan, G., Senthil Kumar, D. & Jagadeesan, L. (2010). Haematology and biochemical parameters of different feeding behavior of teleost fishes from Vellar estuary, India. Comp Clin Pathol http://dx.doi.org/10:1007/s00580-011-1259-7 .
Singh, B.P. & Tandon, P.K., (2009). Effect of river water pollution on hematological parameters of fish, Wallago attu. Res. Environ. Life Sci,.2, 211–214.
Sorour, Sh., & Hamouda, A.H. (2019). Prevalence of nematode infestation in Clarias gariepinus from El-Burullus Lake and Lake Nasser, Egypt. Iraqi Journal of veterinary Sciences, 33(2), 181-188.
Smith, C.G., Lewis, W.M., & Kaplan, H.M. (1952). A Comparative morphologic and physiologic study of fish blood. Prog. Fish Cult., 14, 169 – 172.
Teugels, G.G., Breine, J.J. &Thys Van den Audernaerde, D.F.E. (1984). Channidae. In: J. Daget, J.P. Gosse and D.F.E. Thys van den Anderaerde (Eds) Checklist of the Freshwater Fishes of Africa (CLOFFA). ORSTOM Paris and MRAC,Tervuren: 288–290.
Teugels, G.G., Reid, G.M. & King, R.P. (1992). Fishes of the Cross-River Basin (Cameroon, Nigeria) taxonomy, zoogeography, ecology and conservation: Tervuren, Belgique, Muse Royal de LAfrique Central, Annales Sciences Zoologiques, 266: 132.
The World Book Encyclopedia (1994). Volume 16, p.491. ISBN 0.7166-0094-3. USA Witeska, M. (2005); Stress in fish-haematological and immunological effects of heavy metals, Electronic of Icthyology, 1, 35 – 41.