Aim: In this article, we evaluated the possible cardiac protective effects of silymarin on valproic acid-induced heart injury byhistological and biochemical parameters in rat heart.Material and Methods: The experiment was performed with 21 Sprague Dawley male rats. Rats were divided into three groups: group1; control, group 2; valproic acid, group 3; valproic acid + silymarin. The groups received valproic acid 500 mg/kg/day per os (p.o.)and silymarin 100 mg/kg/day p.o. for 14 days except the control group.Results: Silymarin treatment decreased the levels of lactate dehydrogenase, creatine kinase isoenzyme MB, alanine aminotransferaseand aspartate aminotransferase significantly (p < 0.05). In addition, the increase of malondialdehyde level and decrease of glutathionelevel by valproic acid were significantly suppressed by silymarin in heart tissue (p < 0.05). Histologically, the amount of heart injurywas significantly lower in valproic acid + silymarin group and histopathological findings were decreased in valproic acid + silymaringroup compared to valproic acid group (p < 0.005).Conclusion: In this study, it was observed that silymarin has a curative effect on valproic acid induced heart damage. In this sense,we believe that our study will be usefull for other studies which will be study with silymarin and valproic acid.