Hilal ÖZKUL, Özgen Alpay ÖZBEK, Hüseyin ÇOBAN, Zeynep GÜLAY

Dokuz Eylül Üniversitesi hastanesinde 2003-2006 yıllarında üretilen haemophilus influenzae suşlarının antibiyotik duyarlılıkları

Haemophilus influenzae; menenjit, sepsis, çeşitli doku infeksiyonları ve toplum kökenli pnömonilerin en önemli etkenlerindendir. Bu çalışmada 2003-2006 yıllarında laboratuvarımıza gönderilen çeşitli klinik örneklerden izole edilen 289 H.influenzae suşunun antibiyotik duyarlılıkları CLSI standartlarına uyularak disk difüzyon yöntemi ile araştırılmış, beta-laktamaz aktivitesi nitrosefin hidrolizi yöntemi ile belirlenmiştir. Söz konusu yıllar içinde direnç oranlarına bakıldığında 2003 yılı izolatlarının hiçbirinde beta-laktamaz aktivitesi saptanmamışken sonraki yıllarda beta-laktamaz pozitif suşların arttığı, 2005 yılında % 8’e ulaştığı, 2006 yılında ise % 7 oranında bulunduğu izlenmiştir. Benzer şekilde, trimetoprim-sulfametoksazol direnç oranları da % 29’dan (2003) % 36’ya (2006) yükselmiştir. İzolatların tümü değerlendirildiğinde ampisiline % 5.9, trimetoprim-sulfametoksazole % 31.8 direnç saptanmış, suşların hiçbirinde sefotaksim, sefuroksim, amoksisilin-klavulanat direnci görülmemiştir. Benzer şekilde, beta-laktamaz negatif ampisiline dirençli (BLNAR) izolat varlığı saptanmamıştır

Antimicrobial susceptibility patern of haemophilus influenzae strains ısolated during 2003-2006 at Dokuz Eylül University Hospital

Haemophilus influenzae is one of the most important causes of meningitis, sepsis, various tissue infections and community acquired pneumonia. In this paper antimicrobial susceptibilities of 289 H.influenzae strains isolated from various clinical samples between 2003-2006 were evaluated by disk diffusion method according to the recommendations of CLSI and beta- lactamase activity was investigated by nitrocefin hydrolysis test. Beta-lactamase activity was not detected in 2003 isolates. The percentage of beta-lactamase positive strains increased to 8 % and 7 % in 2005 and 2006, respectively. Similarly, resistance rates to trimethoprim-sulfamethoxazole increased from 29 % (2003) to 36 % (2006). When all isolates were evaluated, resistance rates for ampicillin and trimethoprimsulfamethoxazole were 5.9 % and 31.8 %. None of the isolates were resistant to cefotaxime, cefuroxime, amoxicillin-clavulanate. Similarly, beta-lactamase negative ampicillin resistant (BLNAR) isolates were not detected.

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