Tuba PARLAK AK, Burcu GÜL BAYKALIR, Neşe BAŞAK, Pınar TATLI SEVEN, Mine YAMAN, İsmail SEVEN

Ratlarda bakır fazlalığına karşı yem tüketimi, antioksidan durum ve karaciğer ile böbrek dokularının histolojik parametreleri üzerine propolis ve fluniksin meglümin'in koruyucu etkileri

Bu araştırma, aşırı bakıra maruz bırakılmış sıçanlarda propolis ve fluniksin meglümin’in, yem tüketimi, antioksidan durum ile karaciğer ve böbrek dokularındaki histolojik parametreler üzerindeki koruyucu etkilerini belirlemek amacıyla yapıldı. Araştırmada bireysel olarak barındırılan 36 adet erkek Sprague-Dawley sıçan kullanıldı. Hayvanlar rastgele; kontrol, bakır sülfat (500 mg/kg BW/gün, gavaj), fluniksin meglümin (2,2 mg/kg BW/gün, ip), propolis (100 mg/kg BW/gün, gavaj), bakır sülfat+fluniksin meglümin (500 mg/kg BW/gün bakır sülfat, gavaj ve 2,2 mg/kg BW/gün fluniksin meglümin, ip) ve bakır sülfat+propolis (500 mg/kg BW/gün bakır sülfat ve 100 mg/kg BW/gün propolis, gavaj) olarak altı gruba ayrıldı. Çalışma, bakır+propolis grubunun vücut ağırlığındaki değişimin bakır grubu ile karşılaştırıldığında önemli ölçüde iyileştiğini göstermiştir (P

The protective effects of propolis and flunixin meglumine on feed intake, antioxidant status and histological parameters in liver and kidney tissues against excess copper in rats

This experiment was conducted to determine the protective effects of flunixin meglumine and propolis on feed intake, antioxidant status and histological parameters in kidney and liver tissues in rats exposed to excessive copper. In this study individually housed thirty-six male Sprague-Dawley rats were used. Animals were randomly divided into six groups; control, copper sulphate (500 mg/kg BW/day, gavage), flunixin meglumine (2.2 mg/kg BW/day, ip), propolis (100 mg/kg BW/day, gavage), copper sulphate+flunixin meglumine (500 mg/kg BW/day of copper sulphate by gavage and 2.2 mg/kg BW/day of flunixin meglumine, ip) and copper sulphate+propolis (500 mg/kg BW/day of copper sulphate and 100 mg/kg BW/day of propolis by gavage). The study demonstrated that body weight change in the copper sulphate+propolis group significantly ameliorated in comparison with copper group (P

PDF

___

1. Abu-Zinadah OA, Hussein HK, Elshal MF, et al. (2012): Effect of excess dietary copper on proliferation and differentiation of the proerythroblasts and erythrocytes in rats. Afr J Biotechnol, 11, 6187-6191.
2. Aebi H (1984): Catalase in vitro methods in enzymology. In: Willam BJ, editor. Methods in enzymology. New York, USA: Academic Press; 1984. pp. 121-126.
3. Ahn MR, Kumazawa S, Usui Y (2007): Antioxidant activity and constituents of propolis collected in various areas of China. Food Chem, 101, 1383-1392.
4. Apak R, Guclu K, Ozyurek M, et al. (2004): Novel total antioxidantcapacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem, 52, 7970-7981.
5. Babaknejad N, Moshtaghie AA, Shahanipour K (2015): The toxicity of copper on serum parameters related to renal functions in male wistar rats. Zahedan J Res Med Sci, 15, 29-31.
6. Bino RJ, de Vos CHR, Lieberman M, et al. (2005): The light-hyperresponsive high pigment-2dg mutation of tomato: alterations in the fruit metabolome. New Phytol, 166, 427-438.
7. Chiou PWS, Chen KL, Yu Bi (1998): Effect of dietary organic arsenicals and cupric sulfate on copper toxicity, liver accumulation and residue in eggs and excreta of laying hens. Anim Feed Sci Tech, 73, 161-171.
8. Ciftci O, Tanyildizi S, Godekmerdan A (2010): Protective effect of curcumin on immune system and body weight gain on rats intoxicated with 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Immunopharmacol Immunotoxicol, 32, 99-104.
9. Cook NC, Samman S (1996). Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. J Nutr Biochem, 7, 66-76.
10. Ellman GL (1959): Tissue Sulfhydryl groups. Arch Biochem Biophys, 82, 70-77.
11. Ferenci P (2004): Pathophysiology and clinical features of wilson disease. Metab Brain Dis, 19, 229-239.
12. Fuentealba IC, Aburto EM (2003): Animal models of copper-associated liver disease. Comp Hepatol, 2, 5.
13. Gaetke LM, Chow CK (2003): Copper toxicity, oxidative stress and antioxidant nutrients. Toxicology, 189, 147-163.
14. Gul Baykalir B, Tatli Seven P, Gur S, et al. (2016): The effects of propolis on sperm quality, reproductive organs and testicular antioxidant status of male rats treated with Cyclosporine-A. Anim Reprod, Belo Horizonte, 13, 105-111.
15. Halliwell B, Gutteridge JMC (2007): Oxygen is a toxic gas – an introduction to oxygen toxicity and reactive species. In: Halliwell B, Gutteridge JMC, editors. Free radicals in biology and medicine. New York, USA: Oxford University Press; 2007. pp. 1-29.
16. Kabak YB, Gülbahar MY (2013): Sıçanlarda deneysel bakır zehirlenmesinde karaciğer ve böbrek dokularında apoptozisin belirlenmesi. Ankara Üniv Vet Fak, 60, 39-45.
17. Kim D, Jeong SW, Lee CY (2003): Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem, 81, 321-326.
18. Kodama H, Fujisawa C (2009): Copper metabolism and inherited copper transport disorders: molecular mechanisms, screening and treatment. Metallomics, 1, 42-52.
19. Kornegay ET, van Heugten PHG, Lindemann MD, et al. (1989): Effects of biotin and high copper levels on performance and immune response of weanling pigs. J Anim Sci, 67, 1471-1477.
20. Kumar S, Mishra A, Pandey AK (2013): Antioxidant mediated protective effect of Parthenium hysterophorus against oxidative damage using in vitro models. BMC Complement Altern Med, 13, 120.
21. Kumar S, Pandey AK (2013): Phenolic content, reducing power and membrane protective activities of Solanum xanthocarpum root extracts. Vegetos, 26, 301-307.
22. Lee S, Kim YJ, Kwon S (2009): Inhibitory effects of flavonoids on TNF-α-induced IL-8 gene expression in HEK 293 cells. BMB reports, 42, 265-270.
23. Lim H, Jin JH, Park H, et al. (2011): New synthetic anti-inflammatory chrysin analog, 5,7-dihydroxy-8-(pyridine-4yl) flavone. Eur J Pharmacol, 670, 617-622.
24. Liu JY, Yang X, Sun XD, et al. (2016): Suppressive effects of copper sulfate accumulation on the spermatogenesis of rats. Biol Trace Elem Res, 174, 356-361.
25. Lowry OH, Rosebrough NJ, Farr AL, et al. (1951): Protein measurement with the folin phenol reagent. J Biol Chem, 193, 265-275.
26. Matsuno T (1995): A new clerodane diterpenoid isolated from propolis. Z Naturforsch, 50C, 93-97. 27. Meek IL, van de Laar MAFJ, Vonkeman HE (2010): Non-steroidal anti-inflammatory drugs: an overview of cardiovascular risks. Pharmaceuticals (Basel), 3, 2146-2162.
28. Mladenović JM, Paunović MG, Matić MM, et al. (2014): Copper-induced changes of lipid peroxidation and hemato-biochemical parameters in rat blood: protective role of flavonoids. Arch Biol Sci Belgrade, 66, 1271-1279.
29. Mouithys-Mickalad AML, Zheng SX, Deby-Dupont GP, et al. (2000): In vitro study of the antioxidant properties of non steroidal anti-inflammatory drugs by chemiluminescence and electron spin resonance (ESR). Free Radic Res, 33, 607-621.
30. Ohkawa H, Ohishi N, Yagi K (1979): Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351-358.
31. Ozcelik D, Uzun H (2009): Copper intoxication; Antioxidant defenses and oxidative damage in rat brain. Biol Trace Elem Res, 127, 45-52.
32. Rana SVS (2008): Metals and apoptosis: Recent developments. J Trace Elem Med Bio, 22, 262-284. 33. Rice-Evans CA, Miller NJ, Bolwell PG, et al. (1995): The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res, 22, 375-383.
34. Ross MH, Reith EJ, Romrell LJ (1989): Histology: a text and atlas. 2nd ed. Williams and Wilkins: Baltimore.
35. Seven I, Gul Baykalir B, Tatli Seven P, et al. (2014): The ameliorative effects of propolis against cyclosporine A induced hepatotoxicity and nephrotoxicity in rats. Kafkas Univ Vet Fak, 20, 641-648.
36. Sun Y, Oberley LW, Li YA (1988): Simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500.
37. Tapiero H, Townsend DM, Tew KD (2003): Trace elements in human physiology and pathology. Copper. Biomed Pharmacother, 57, 386-398.
38. Tatli Seven P (2008): The effects of dietary Turkish Propolis and vitamin C on performance, digestibility, egg production and egg quality in laying hens under different environmental temperatures. Asian-Aust J Anim Sci, 21, 1164-1170.
39. Tatli Seven P, Seven I, Yilmaz M, et al. (2008): The effects of Turkish Propolis on growth and carcass characteristics in broilers under heat stress. Anim Feed Sci Tech, 146, 137-148.
40. Tatli Seven P, Yilmaz S, Seven I, et al. (2012): Effects of propolis in animals exposed oxidative stress (Chapter 13). In: Lushchak VI, editor. Oxidative stress-environmental induction and dietary antioxidants. Rijeka, Croatia: InTech; 2012. pp. 267-288.
41. Velioglu YS, Mazza G, Gao L, et al. (1998): Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem, 46, 4113-4117. 42. Zhang SS, Noordin MM, Rahman SO (2000): Effects of copper overload on hepatic lipid peroxidation and antioxidant defense in rats. Vet Hum Toxicol, 42, 261-264.
43. Zu Y, Li C, Fu Y, et al. (2006): Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD. J Pharma Biomed Anal, 41, 714-719.