Enver BEYTUT, Engin KILIÇ

Buzağılarda kongenital deri melanomlarının histopatolojik ve immunohistokimyasal olarak değerlendirilmesi (3 vaka)

Vücutlarının farklı bölgelerindeki deri üzerinde siyah kitle bulunan 3 erkek buzağı klinik olarak muayene edildi. Kitlelerin doğumla beraber görüldüğü ve zamanla büyüdükleri bildirildi. Tüm kitleler rutin cerrahi yolla alındı ve histopatolojik olarak değerlendirildi. Tümör kitlelerinin yuvarlak veya iğsi çekirdekli hücrelerden oluştuğu ve sitoplazmalarının melanin granülleri ile dolduğu görüldü. Mitoz oranı yüksek büyütmede ortalama 5-7 olarak belirlendi. Neoplastik melanositlerde Melan A, HMB-45, S-100, NSE ve vimentin için sitoplazmik reaksiyon görüldü. Yine tümör hücreleri Ki-67 nükleer pozitif reaksiyon gösterdi. Nükleer pleomorfizim ve yüksek mitotik oranla birlikte, tümör hücrelerinin melanositik markırlara karşı pozitif reaksiyonu kitlelerin melanom olduğunu göstermiştir. Bununla birlikte, melanosit kökenli tümörlerin biyolojik karakterinin belirlenmesinde ileri görüntüleme teknikleri ile birlikte histopatolojik ve immunohistokimyasal değerlendirmeler yapılmalıdır.

Histopathological and immunohistochemical evaluation of congenital cutaneous melanomas in calves (3 cases)

Three male calves with a dark mass, hanging down on the skin at various parts of the body, were presented for clinical examination. All masses were present at the birth of the calves and their size increased with time. The masses were extirpated by a routine surgical operation and presented for histopathological examination. Tumour cells having cytoplasmic melanin granules showed a round or spindle-shaped nuclei. The mean mitotic rate was 5-7 at a high power field. Neoplastic melanocytes displayed an evident cytoplasmic immunolabeling to Melan A, HMB-45, S-100, NSE, and Vimentin. Tumour cells showed a nuclear positivity for Ki-67. Nuclear pleomorphism and a high mitotic rate along with the positive reaction of neoplastic cells to the melanocytic markers were indicative of melanoma. Nevertheless, neoplasms originating from melanocytes always need advanced imaging techniques along with histopathological and immunohistochemical examination while assessing biological behavior of the tumour.

PDF

___

1. Aydoğan A, Haligür M, Özmen O (2013): Hepatic malignant melanoma in a goat, primary or metastatic? Isr J Vet Med, 68, 124-127.
2. Brito MF, Franca TN, Jabour FF, et al. (2009): Metastasizing oral melanoma in a cow. Ciência Rural, Santa Maria, 39, 1248-1252.
3. Canpolat İ, Yaman İ, Günay C (2007): A case of primary intraocular malignant iris melanoma in an Akkaraman sheep. Revue Méd Vét, 58, 171-173.
4. Chandrashekaraiah GB, Ballari SV, Manjunatha K, et al. (2014): Malignant melanoma in a Hallikar bullock. Int J Vet Sci, 3, 65-67.
5. Garma-Avina A, Valli VE, Lumsden JH (1981): Cutaneous melanomas in domestic animals. J Cutan Pathol, 8, 3-24.
6. Goldschmidt MH, Hendrick MJ (2002): Tumors of the skin and soft tissues. 45-117. In: DJ Meuten (ed), Tumors in Domestic Animals. 5th (edt), Iowa State Press, Ames, Iowa.
7. Gülbahar MY, Özak A, Kabak YB, et al. (2016): Oral metastatic melanoma with neurosarcomatous transformation in a dog. Ankara Univ Vet Fak Derg, 63, 89-92.
8. Hsu S, Raine L, Fanger H (1981): Use of avidin–biotin peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem, 29, 577-580.
9. Javanbakht J, Sasani F, Adibhashemi F, et al. (2014): Comparative histopathological diagnosis of cutaneous melanoma by H&E, special staining and immunohistochemical methods against cutaneous squamous cell carcinoma in horse and bovine. J Bioanal and Biomed, 6, 19-23.
10. Koenig A, Wojcieszyn J, Weeks BR, et al. (2001): Expression of S100a, Vimentin, NSE, and Melan A/MART-1 in seven canine melanoma cell lines and twenty-nine retrospective cases of canine melanoma. Vet Pathol, 38, 427-435.
11. Mesaric M, Zadnik T, Cerne M (2002): Malignant melanoma in a cow. Acta Vet-Beograd, 52, 59-64.
12. Millanta F, Fratini F, Corazza M, et al. (2002): Proliferation activity in oral and cutaneous canine melanocytic tumours: correlation with histological parameters, location, and clinical behavior. Res Vet Sci, 73, 45-51.
13. Miller MA, Weaver AD, Stogsdill PL, et al. (1995): Cutaneous melanocytomas in 10 young cattle. Vet Pathol, 32, 479-484.
14. Moulton JE (1990): Tumors in Domestic Animals. University of California Press, Los Angeles, USA.
15. Pravettoni D, Ordobazari M, Beineke A (2003): Congenital melanoma in a heifer. Dtsch Tierarztl Wochenschr, 110, 34-36.
16. Ramos-Vara JA, Miller MA, Johnson GC, et al. (2002): Melan A and S100 protein immunohistochemistry in feline melanomas: 48 Cases. Vet Pathol, 39, 127-132.
17. Ramos-Vara JA, Beissenherz ME, Miller MA, et al. (2000): Retrospective study of 338 canine oral melanomas with clinical, histologic, and immunohistochemical review of 129 cases. Vet Pathol, 37, 597-608.
18. Ramos-Vara JA, Miller MA (2011): Immunohistochemical identification of canine melanocytic neoplasms with antibodies to melanocytic antigen PNL2 and tyrosinase: comparison with melan A. Vet Pathol, 48, 443-450.
19. Rezaie A, Golshahi H, Hajikolaie MRH (2013): Epibulbar melanocytoma in a goat. Turk J Vet Anim Sci, 37, 479-481.
20. Sakai H, Yanai T, Kawakami S, et al. (2001): Malignant melanoma of the palpebral conjunctiva in a captive fallow deer. J Wildlife Dis, 37, 816-819.
21. Scandrett B, Wobeser G (2004): Malignant melanoma in a captive red deer (Cervus elaphus elaphus). J Wildlife Dis, 40, 808-810.
22. Schuh JCL (1989): Congenital intraocular melanoma in a calf. J Comp Pathol, 101, 113-116.
23. Smith SH, Goldschmidt MH, Mcmanus PM (2002): A comparative review of melanocytic neoplasms. Vet Pathol, 39, 651-678.
24. Vadalia JV, Fefar DT, Patel PB (2016): Surgical management of malignant melanoma in Kankrej cow. Intas Polivet, 17, 98-99.
25. Yager JA, Scott DW (1993): The skin and appendages. 531-738. In: Jubb KVF, Kennedy PC, Palmer N, (Eds), Pathology of Domestic Animals. 4th ed. vol. 1, Academic press, San Diego, CA.