Emine ARIK, Habibullah DOLGUN, Pınar KURU BEKTAŞOĞLU, Julide ERGİL, Banu COŞKUN YILMAZ, Çağhan TÖNGE, Özden Çağlar ÖZTÜRK, Levent GÜRSES, Bora GÜRER

Rat Travmatik Beyin Hasarı Modelinde Paeonol’ün Nöroprotektif Etkisi

Amaç: Travmatik beyin hasarı (TBH), oksidatif stres ve hücresel hasara neden olan apoptozu indükleyen temel bir sakatlık ve ölüm nedenidir. Bazı hayvan modelleri paeonolün güçlü bir antioksidan, antiapoptotik ve nöroprotektif madde olduğunu göstermiştir. Bu çalışma, paeonolün rat TBI modelinde olası nöroprotektif etkilerini araştırmayı amaçlamıştır. Gereç ve Yöntemler: Otuz iki erkek rat dört gruba ayrıldı: kontrol, travma, taşıyıcı ve paeonol. Travma, taşıyıcı ve paeonol gruplarında kapalı kafa travması ağırlık düşürülerek uygulandı. Taşıyıcı (serum fizyolojik) veya paeonol (50 mg/kg) 15 gün boyunca premedikasyon olarak oral yoldan uygulandı. Beyin örnekleri travmadan 24 saat sonra alındı. Serebral korteksin histomorfolojik değerlendirmesi elektron ve ışık mikroskopisi kullanılarak yapıldı. Bulgular: Histopatolojik incelemede, paeonol grubunda TBH kaynaklı serebral korteks hasarının daha az olduğu görüldü. Sonuç: Paeonol, TBI’ya karşı nöroprotektif ve antiödematöz etkiler sergilemiştir.

Neuroprotective Effect of Paeonol in the Rat Model of Traumatic Brain Injury

Objectives: Traumatic brain injury (TBI) is a major cause of disability and mortality that induces oxidative stress and apoptosis causing cellulardamage. Several animal models have shown paeonol to be a powerful antioxidant, antiapoptotic, and neuroprotective substance. This study aimedto investigate possible neuroprotective effects of paeonol in a rat TBI model.Materials and Methods: Thirty-two male rats were divided into four groups: control, trauma, vehicle, and paeonol groups. Trauma, vehicle, andpaeonol groups were subjected to closed-head, contusive weight-drop injuries. The vehicle (saline) or paeonol (50 mg/kg) was orally administered aspremedication for 15 days. Brain samples were obtained 24 hours after trauma. Histomorphological evaluation of the cerebral cortex was performedusing electron and light microscopy.Results: Histopathological examination revealed that the TBI-induced cerebral cortex damage was less in the paeonol group.Conclusion: Paeonol exhibited neuroprotective and anti-edematous effects against TBI.

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