Comparing the different fixatives for examination of liver tissue ultrastructure
Amaç: Enfeksiyonlar ve depo hastal ı kları gibi karaci ğ er patolojilerinde dokunun ince yap ı düzeyinde incelenmesi önem ta șı maktad ı r. Bu çal ı ș ma; çe șitli tespit s ı v ı ları n ı n etkinli ğ ini ve birbirineolan üstünlü ğ ünü ara ștı rarak, dokunun de ğ i ș ken laboratuvar yöntemlerinden etkilenmesini enaza indirmek üzere planlanm ı ștı r.Yöntemler: Çal ı ș mada 5 adet Wistar Albino rat kullan ı ld ı . Ratları n %4 paraformaldehitleperfüzyonundan sonra dokular ç ı karı ld ı ve dört farkl ı tespit solusyonuyla takip edildi: I- Trumps solüsyonu %4 paraformaldehit (P) ve %1 gluteraldehit (G ),II- % 2 PFA ve % 2.5 G, 2,5 mM CaCl içeren, III- % 2 PFA ve % 2,5 G, IV- % 2,5 G. I, II, III. Yöntemlerde fosfat tampon kullan ı l ı rken, IV. yöntemde kakodilat tamponlu fiksatif kulla-n ı ld ı . Di ğ er i șlemler bilinen elektron mikroskobi takip yöntemiyle sürdürüldü ve geçirimli elekt-ron mikroskobunda incelendi. Örnekler skorlanarak istatistiksel olarak değ erlendirildi.Bulgular: Plazma zarı ve hücreler arası ba ğ lantı kompleksleri grup I ve II de daha iyi korundu.Grup IV mitokondriyon yap ı s ı n ı n en iyi izlendi ğ i grup olarak belirlendi. İ nce yap ı n ı n de ğ erlendi-rildi ğ i be ș kritere göre yap ı lan skorlama ve istatistiksel sonuçlara göre karaci ğ er için en iyi tespitsolusyonu s ı ralaması I, IV, II ve III olarak belirlendi.Sonuç: Uygulanan di ğ er fiksatiflere göre de ğ erlendirildi ğ inde Trumps solusyonun karaci ğ erdokusu tespitinde daha iyi oldu ğ u bulundu.
Farklı fiksatiflerin karaciğer dokusunda ince yapı düzeyinde etkilerinin karșılaș tırılması
Aim: It is important to examine liver tissue ultrastructure in pathologies of infections andstorage diseases. The aim of this study was to assess the effects and advantages of variousfixatives on liver ultrastructure and to minimize the methodological errors during the fixation ofliver tissue for transmission electron microscopy.Materials and Methods: Five Wistar rats were used. After the rats were perfused with 4%paraformaldehyde (PFA), tissues were extracted and immersed with four different fixationtechniques:I- Trump s solution 4% paraformaldehyde (PFA) and 1% glutaraldehyde (G)II- 2% F and 2.5% G with 2.5 mM CaClIII- 2% PFA and 2.5% G IV- 2.5% G in 0.1M Cacodylate bufferSamples were embedded, cut, stained and examined with transmission electron microscope.Samples were scored and analyzed. Results: The plasma membrane and cellular junctions were better preserved in group I and II.The best mitochondria structure was in group IV. The total score rank from the highest to thelowest was as follows: Group I, IV, II, and III.Conclusion: The results for the samples fixed in Trump s fixative were better than thoseachieved by other fixing agents.
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- Başlangıç: 1947
- Yayıncı: Erkan Mor
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Comparing the different fixatives for examination of liver tissue ultrastructure
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