TETRASİKLİN VE İNFEKSİYÖZ HEPATİT ŞARTLARI ALTINDA 1,2,4-TRİAZOL TÜREVLERİNİN KARACİĞER KORUYUCU AKTİVİTELERİNİN ARAŞTIRILMASI

Amaç: Bu çalışmanın amacı tavuklarda oluşturulan tetrasiklin ve infeksiyöz hepatit modellerinde potasyum 2-((4-amino-5-(morfolinometil)-4H-1,2,4-triazol-3-il)tiyo)asetatın karaciğer koruyucu özelliklerini çalışmaktır. Gereç ve Yöntem: Kan ve karaciğer serumunun biyokimyasal analizi Cobb 500 cinsi sağlıklı etçi civcivlerde yapılmıştır. Belirlenen koşullara uygun olarak aynı mikroklima parametreleri için tüm civciv grupları aynı oda içinde farklı kafeslerde ayrı olarak tutulmuştur. Sonuç ve Tartışma: Civcivlerde tetrasiklin ile oluşturulan hepatit modelindeki çalışmanın sonucu olarak potasyum 2-((4-amino-5- (morfolinometil)-4H-1,2,4-triazol-3-il)tio)asetat’ın belirlenen karaciğer koruyucu etkileri referans ilaç olan Thiotriazolin’den daha aşağıda bulunmamıştır. Biyokimyasal çalışmanın sonuçları, yukarıda belirtilen bileşiğin Saroflox antibiyotik ile kombinasyonunun, klasik antibiyotik Enrofloxacin ile potasyum 2-((4-amino-5- (morfolinometil)-4H-1,2,4-triazol-3-il)tio)asetat’ın kombinasyonunun karaciğer koruyucu özelliklerini göstermektedir.

Anahtar Kelimeler:

biyokimyasal belirteçler, karaciğer koruyucu aktivite, 1.2.3-triazol

THE RESEARCH OF 1,2,4-TRIAZOLE DERIVATIVES HEPATOPROTECTIVE ACTIVITY UNDER TETRACYCLINE AND INFECTIOUS HEPATITIS

Objectives: The purpose of this work is to study the hepatoprotective properties of potassium 2-((4-amino-5- (morpholinomethyl)-4H-1,2,4-triazole-3-yl)thio)acetate on tetracycline and infectious hepatitis models in chickens. Materials and Methods: Biochemical examination of blood and liver serum was carried out on intact broiler chickens Cobb 500 cross. All groups of chickens were kept separate in different cells in the same room for the same microclimate parameters in accordance with the established requirements. Result and Discussion: As a result of research on the model of hepatitis in chickens caused by tetracycline, established hepatoprotective effect of potassium compound 2-((4-amino-5-(morpholinomethyl)-4H-1,2,4-triazole-3-yl)thio)acetate, which is not inferior to the referent drug - Thiotriazolin®. Based on the results of the biochemical study, hepatoprotective properties of the combination of the above compound with the antibiotic Saroflox®, combination of potassium 2-((4-amino-5- (morpholinomethyl)-4H-1,2,4-triazole-3-yl) thio) acetate with classical antibiotic Enrofloxacin® on the model of infectious hepatitis.

Keywords:

1.2.4-triazole, hepatoprotective activity, biochemical indicators,

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1. World Health Organization. (2017). Global hepatitis report 2017. World Health Organization.
2. Popovich, V. P. (2011) Research of the Range of Hepatoprotectors in the Pharmaceutical Market of Ukraine. Pharmacology and Drug Toxicology, 1(20), 75-81.
3. Popovich, V. P., Gromovik, B. P., & Glukhovsky, P. V. (2012). Market segment of hepatoprotectors in group and individual measurements. Pharmacology and Drug Toxicology, 1(26), 72-76.
4. Reyes-Arellano, A., Gómez-García, O., & Torres-Jaramillo, J. (2016). Synthesis of Azolines and Imidazoles and their Use in Drug Design. Med. Chem.(Los Angeles), 6, 561-570.
5. Rud, A. M., Kaplaushenko, A. G., Pruglo, E. S., & Frolova, Y. S. (2017). Hepatoprotective activity of 1, 2, 4-triazole-3-thione derivatives, which contains on C5-atomic carbon hydroxy (phenyl) methyl dependent. Farmatsevtychnyi zhurnal, (5-6), 55-63.
6. Mohammed, G. J., & Hameed, I. H. (2018). Pharmacological Activities: Hepatoprotective, Cardio Protective, Anti-cancer and Anti-Microbial Activity of (Raphanus Raphanistrum Subsp. Sativus): A review. Indian Journal of Public Health, 9(3), 212-217. 7. Udom, G. J., Yemitan, O. K., Umoh, E. E., Mbagwu, H. O., Ukpe, E. E., & Thomas, P. S. (2018). Hepatoprotective Properties of Ethanol Seed Extract of Citrus paradisi Macfad (Grape Fruit) Against Paracetamol-Induced Hepatotoxicity in Wistar Rats. Journal of Herbal Drugs (An International Journal on Medicinal Herbs), 8(4), 219-227.
8. Mari, G., Catalani, S., Antonini, E., De Crescentini, L., Mantellini, F., Santeusanio, S., & Palma, F. (2018). Synthesis and biological evaluation of novel heteroring-annulated pyrrolino-tetrahydroberberine analogues as antioxidant agents. Bioorganic & medicinal chemistry, 26(18), 5037-5044.
9. Vyshtakalyuk, A. B., Nazarov, N. G., Zobov, V. V., Abdulkhakov, S. R., Minnekhanova, O. A., Semenov, V. E., & Reznik, V. S. (2017). Evaluation of the hepatoprotective effect of L-ascorbate 1-(2-hydroxyethyl)-4, 6-dimethyl-1, 2-dihydropyrimidine-2-one upon exposure to carbon tetrachloride. Bulletin of experimental biology and medicine, 162(3), 340-342.
10. Sachdeva, M., Chadha, R., Kumar, A., Karan, M., Singh, T., & Dhingra, S. (2015). Hepatoprotective effect of trimethylgallic acid esters against carbon tetrachloride-induced liver injury in rats. Hepatoprotective effect of trimethylgallic acid esters against carbon tetrachloride-induced liver injury in rats. Indian Journal of Experimental Biology,53, 803-809.
11. Tiotriazolin® tablets - instructions, analogues, composition, indications, reviews. Arterium Corporation. Reference http://compendium.com.ua/info/7043/tiotriazolin-sup-sup-. Published 2018. Accessed November 20, 2018.
12. Bushueva, I., Parchenko, V., Shcherbyna, R., Safonov, A., Kaplaushenko, A., Gutyj, B., & Hariv, I. (2017). Tryfuzol-new original veterinary drug. J. Fac. Pharm. Ankara/Ankara Ecz. Fak. Derg, 41(1), 42-49.
13. Shcherbyna, R. O., Panasenko, O. I., & Knysh, Y. H. (2016). The studying of antioxidant activity of salts 2-((4-R-3-(morpholinomethylen)-4H-1, 2, 4-triazole-5-yl)thio)acetic acids. Ukraïns’ kij bìofarmacevtičnij žurnal, 1 (42), 37-48.
14. Stalnaya, I.D., Garishvili, T.G. (1977). Method for the Determination of Malonic Dialdehyde Using Thiobarbituric Acid. Modern methods in biochemistry, 66-68.
15. Lutsik, B.D. (2011). Clinical laboratory diagnostics. Medicine: Kiev.
16. Burtis, C. A., Ashwood, E. R., & Bruns, D. E. (2012). Tietz textbook of clinical chemistry and molecular diagnostics-e-book. Elsevier Health Sciences.
17. Dembińska-Kieć, A. & Naskalski, J. W. (Eds.). (2017). Diagnostyka laboratoryjna z elementami biochemii klinicznej. Edra Urban & Partner.
18. Beutler, E.D., Duron Q. & Kelly B.M. (1963). A Colorimetric Method for the Determination of Serium Glutamic Oxalatic and Glutaminic Pynovic Transaminases. Journal Laboratories Clinical Medicine, 61, 882.
19. Korolyuk, M. A., Ivanova, L. K., Mayorova, I. G. & Tokareva, V. A. (1988). Method For The Determination of Catalase Activity. Clinical laboratory diagnosis, 4, 44-47.
20. Khalafyan, A.A. (2007). Statistica 6. Statistical Data Analysis. Textbook; Binom-Press: Moskow.
21. Shcherbyna, R. O., & Vashchyk, Y. V. (2018). Histological study of potassium 2-((4-amino-5-(morpholinomethyl)-4H-1, 2, 4-triazole-3-yl)thio)acetate (PKR-173) corrective influence on the chickens liver state in the condition of tetracycline hepatitis. Zaporožskij Medicinskij Žurnal, 3, 404-412.